Preparation and use method of ratio fluorescent probe for visual distinguishing detection

Abstract

The invention discloses a preparation and use method of a ratio fluorescent probe for visual distinguishing detection. The preparation method specifically comprises the following steps: step 1, preparation of silicon-based carbon quantum dots; step 2, preparation of the nano mesoporous molecularly imprinted material coated with the silicon-based carbon quantum dots; step 3, performing surface modification on the nano mesoporous molecularly imprinted material; and step 4, preparing the ratio fluorescent probe based on the nano mesoporous molecularly imprinted material. The use method comprises the following steps: (1) diluting tetracycline or oxytetracycline into solutions with different gradient concentrations; (2) dispersing the prepared ratiometric fluorescent probe based on the nano mesoporous molecularly imprinted material in ultrapure water; (3) adding a Tris-HCl buffer solution with the pH value of 8 into a quartz cuvette, uniformly mixing the Tris-HCl buffer solution and the solution, and respectively dropwise adding the tetracycline solution or the oxytetracycline solution in the step (1) for fluorescence determination; the main substrate is the silicon dioxide molecular imprinting layer with the mesoporous structure, the method is reliable, mass production can be carried out, and the probe is widely applied to the society.

Description

technical field [0001] The invention relates to the technical field of preparing nanocomposite materials, in particular to a method for preparing and using a ratio fluorescent probe for visual discrimination and detection. Background technique [0002] Both tetracycline and oxytetracycline are the most widely used antibiotics in the tetracycline class, commonly used to treat human and animal infections caused by Gram-positive and Gram-negative bacteria. Due to their significant antibacterial effects, tetracycline antibiotics are often used as feed additives, used to prevent and treat diseases, and promote animal growth and development during animal feeding. However, the irrational use of tetracycline antibiotics, including tetracycline (TC) and oxytetracycline (OTC), may lead to the accumulation of tetracycline antibiotics. Although antibiotics have helped humans defeat many diseases caused by bacteria, more than 10% of the human population also suffers from side effects of...

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Problems solved by technology

At the same time, the detection of TC / OTC by a single europium ion will be interfered by other organic substances, and the specificity is poor; most importantly, it is impossible to distinguish the same class of tetracycline substances with similar structures, and its chemical and thermal stability have always been difficult to solve problems; secondly, products are often not mass-produced, which limits their practical application range

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