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A method for high-efficiency polygeneration of macroalgae and biochar and capacitor electrodes

A technology of polygeneration and marine biology, which is applied in the field of biomass resource utilization, can solve the problems of chemical pretreatment pollution, etc., and achieve the effect of improving utilization rate, improving recycling rate, and low cost

Active Publication Date: 2022-01-11
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical pretreatment using strong acid, strong alkali or other organic substances to destroy polysaccharides in seaweed cell structure is relatively polluting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Preparation of abalone digestive enzymes and macroalgae raw materials

[0044] The abalones were purchased from the local area, and the abalones were processed with a scalpel to obtain the digestive tissue of the abalones, and the intestinal contents were washed thoroughly, weighed, and 0.1mol / L of 4°C pre-cooled pH value of 3.5 was added at a ratio of 1:4. Acetic acid-sodium acetate buffer, use a mixer to beat intermittently for 2 minutes, cool in an ice bath, ultrasonicate once for 5 seconds, with an intermittent time of 10 seconds, and ultrasonic power at 200W, take it out after 20 minutes of treatment, place it in a refrigerator at 4°C for 5 hours, and use high-speed freezing Centrifuge at 4°C for 30 min at a speed of 9000 r / min, and take the supernatant for later use, which is the crude enzyme solution used in the enzymatic hydrolysis pretreatment. With 1mol / L sodium carbonate solution as solvent, prepare p-nitrophenol standard solution, take 0.6ml crude enzyme...

Embodiment 2

[0050] (1) Preparation of sea urchin digestive enzymes and macroalgae raw materials

[0051]Sea urchins were purchased from the local area, and the digestive tissue of the sea urchins was obtained by using a scalpel, and the intestinal contents were washed thoroughly, weighed, and 0.1 mol / L of 4°C pre-cooled pH value of 3.5 was added at a ratio of 1:4. Acetic acid-sodium acetate buffer, use a mixer to beat intermittently for 2 minutes, cool in an ice bath, ultrasonicate once for 5 seconds, with an intermittent time of 10 seconds, and ultrasonic power at 200W, take it out after 20 minutes of treatment, place it in a refrigerator at 4°C for 5 hours, and use high-speed freezing Centrifuge at 4°C for 30 min at a speed of 9000 r / min, and take the supernatant for later use, which is the crude enzyme solution used in the enzymatic hydrolysis pretreatment. With 1mol / L sodium carbonate solution as solvent, prepare p-nitrophenol standard solution, take 0.6ml crude enzyme solution, 20ml ...

Embodiment 3

[0057] (1) Preparation of abalone digestive enzymes and macroalgae raw materials

[0058] The abalones were purchased from the local area, and the abalones were processed with a scalpel to obtain the digestive tissue of the abalones, and the intestinal contents were washed thoroughly, weighed, and 0.1mol / L of 4°C pre-cooled pH value of 3.5 was added at a ratio of 1:4. Acetic acid-sodium acetate buffer, use a mixer to beat intermittently for 2 minutes, cool in an ice bath, ultrasonicate once for 5 seconds, with an intermittent time of 10 seconds, and ultrasonic power at 200W, take it out after 20 minutes of treatment, place it in a refrigerator at 4°C for 5 hours, and use high-speed freezing Centrifuge at 4°C for 30 min at a speed of 9000 r / min, and take the supernatant for later use, which is the crude enzyme solution used in the enzymatic hydrolysis pretreatment. With 1mol / L sodium carbonate solution as solvent, prepare p-nitrophenol standard solution, take 0.6ml crude enzyme...

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PUM

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Abstract

The invention provides a method for high-efficiency polygeneration of large seaweed and biochar and capacitor electrodes. The method includes the following steps: using enzymatic hydrolysis pretreatment technology, digesting GH9 with high activity in the tissues of abalone or sea urchin Three Digestive Enzymes Cut-β-1,4-Glucanase, GH16β-1,3-Glucanase and GH5_10 Endo-β-1,4-Mannanase Applied to the Pretreatment of Macroalgae Plants , by degrading the cell structure of macroalgae, the yield of bio-oil, biochar and other products after pyrolysis of macroalgae plants is improved, thereby improving the overall utilization rate of macroalgae. And by using the attached digestive enzymes in the solid phase of the enzymatic hydrolysis treatment used for pyrolysis as a high-quality nitrogen source, the nitrogen atoms are transferred to the solid phase products during the pyrolysis process and the nitrogen atoms doped with biochar are obtained to improve the circulation of digestive enzymes utilization rate.

Description

technical field [0001] The invention belongs to the field of biomass resource utilization, and in particular relates to a method for high-efficiency polygeneration of large seaweed, biochar and capacitor electrodes. Background technique [0002] Macroalgae, as a kind of plants with fast reproduction speed and high protein and lipid content, have been studied for the production of biofuels and high-value chemicals in recent years. Due to the presence of cell walls and intercellular structural polysaccharides in macroalgae cells, the conversion rate of macroalgae in thermochemical transformation is reduced, thereby reducing the yield of thermochemical conversion products of macroalgae such as bio-oil and biochar, and increasing the macroalgae Energy consumption during thermochemical conversion. Therefore, it is necessary to select reasonable and efficient pretreatment technology to pretreat macroalgae. At present, the traditional pretreatment technology of macroalgae mainly i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
CPCC10B53/02C10B57/08C11B1/025C11B1/12C10L1/02H01G11/44Y02E50/10
Inventor 王爽凌祺凡闫佳玮汪海文胡栓虎胡亚敏钱黎黎侯靖
Owner JIANGSU UNIV
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