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Efficient cell serum-free culture medium system and application of cell secretions thereof

A technology of cell secretion and cell culture, applied in the application field of cell serum-free medium system and its cell secretion, to achieve the effect of low cost, better quality and best cell secretion products

Inactive Publication Date: 2021-06-04
广州暨大美塑生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] There are many types of commercial culture media available, but according to the instructions provided by the manufacturer, it is generally necessary to cooperate with other types of culture media from the same manufacturer to obtain a better culture effect

Method used

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  • Efficient cell serum-free culture medium system and application of cell secretions thereof
  • Efficient cell serum-free culture medium system and application of cell secretions thereof
  • Efficient cell serum-free culture medium system and application of cell secretions thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1: the composition of culture medium is as follows

[0081] 65 parts by volume of basal medium, 33 parts by volume of serum substitute, and 2 parts by volume of supplement, wherein:

[0082] The basal medium is DMEM basic (1X) (high sugar) from GIBICO Company, product number C11995500BT;

[0083] Serum replacement for KnockOut by GIBICO TM DMEM (1X), Cat. No. 10829018;

[0084] The supplement is PEN STREP GLUTAMINE from Invitrogen Company, product number DXT-10378016.

[0085] The market price for preparing 500 mL of complete medium in Example 1 is about 479 yuan.

Embodiment 2

[0086] Embodiment 2: the composition of culture medium is as follows

[0087] 75 parts by volume of basal medium, 24 parts by volume of serum substitute, and 1 part by volume of supplement, wherein:

[0088] The basal medium is Advanced DMEM / F12 from Thermo Fisher Scientific, Cat. No. 12634010;

[0089] Serum replacement for UltraCULTURE by LONZA TM Serum-free medium, item number 12-725F;

[0090] The supplement is GlutaMAX™ from GIBICO, product number 35050061;

[0091] The market price for preparing 500 mL of complete medium in Example 2 is about 643 yuan.

Embodiment 3

[0092] Embodiment 3: the composition of culture medium is as follows

[0093] 80 parts by volume of basal medium, 18 parts by volume of serum substitute, and 2 parts by volume of supplement, in which:

[0094] The basic medium is DMEM basic (1X) (low sugar) from GIBICO Company, product number C11885500BT;

[0095] Serum replacement for KnockOut by GIBICO TM DMEM (1X), Cat. No. 10829018;

[0096] The supplement is PEN STREP GLUTAMINE from Invitrogen Company, product number DXT-10378016.

[0097] The market price for preparing 500 mL of complete medium in Example 3 is about 454 yuan.

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Abstract

The invention discloses an efficient cell serum-free culture medium system and application of cell secretions thereof. A complete cell culture medium is composed of a basic culture medium, a serum substitute and a supplement. When the cell culture medium of some examples in the invention is used for culturing human umbilical cord-derived stem cells, the time for P0 cells to climb out until the cells reach the passage density is only 10 days; and the cultured P4-generation umbilical cord mesenchymal stem cells are arranged in a regular long shuttle shape and are better in state. The cultured P4-generation umbilical cord mesenchymal stem cells still maintain good differentiation capability, and can be well differentiated into osteoblasts and lipoblasts. The flow type surface marker detection proves that the cells have the specific surface markers of the stem cells and do not have the specific surface markers of the cord blood stem cells. When the cell culture medium in some examples in the invention is used for culturing fibroblasts, the growth rate is better; the aging degree of the cells is lower; and the effect of the cell culture medium is better than that of the existing commercial cell culture medium.

Description

technical field [0001] The invention relates to the field of biology, in particular to an efficient cell serum-free culture medium system and the application of cell secretions thereof. Background technique [0002] Stem cells are a type of cells with self-replication ability and multi-directional differentiation potential. Under certain conditions, they can differentiate into various functional cells and tissues, and have biological functions such as immune regulation, regeneration and repair. Mesenchymal stem cells (MSCs) are adult stem cells derived from the mesoderm, widely present in connective tissue and interstitium of organs throughout the body, and can differentiate into bone, cartilage, fat, Muscle and nerve cells, etc., and have the functions of promoting tissue repair, immune regulation and supporting hematopoiesis, with low immunogenicity, so MSCs are the most promising tissue engineering seed cells for regenerative medicine and immune regulation therapy. [00...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775A61K8/65A61Q19/00
CPCA61K8/65A61Q19/00C12N5/0656C12N5/0665C12N2500/90
Inventor 夏黎明王亮陈钰莹万阳
Owner 广州暨大美塑生物科技有限公司
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