Ultrasonic modified whey protein chelated ferrous iron supplementing agent and preparation method thereof
A whey protein, ultrasonic modification technology, applied in the biological field, can solve the problems of unstable structure, low absorption rate, gastrointestinal tract irritation, etc.
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Embodiment 1
[0022] 1. The preparation method of ultrasonically modified whey protein chelated ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare a whey protein solution with a mass concentration of 40 mg / mL with distilled water, and mix whey protein The solution was ultrasonically modified at an ultrasonic frequency of 20 kHz, an ultrasonic amplitude of 20%, and an ultrasonic time of 5 minutes (every ultrasonic 10 s, stop ultrasonic 5 s), and cooled to room temperature to obtain an ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:1, and then the pH value is adjusted to 6 with HCl and NaOH, and magnetically stirred at 25°C for 20min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a speed ...
Embodiment 2
[0025] 1. The preparation method of ultrasonic modified whey protein ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, and whey protein solution is in Ultrasonic modification was carried out under the conditions of ultrasonic frequency of 20 kHz, ultrasonic amplitude of 30%, and ultrasonic time of 10 min (every ultrasonic 10 s, stop ultrasonic for 5 s), and cooled to room temperature to obtain ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:5, and then the pH value is adjusted to 6 with HCl and NaOH, and magnetically stirred at 25°C for 40min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a ...
Embodiment 3
[0028] 1. The preparation method of ultrasonic modified whey protein ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, and whey protein solution is in Ultrasonic modification was performed under the conditions of ultrasonic frequency of 20 kHz, ultrasonic amplitude of 20%, and ultrasonic time of 15 min (every ultrasonic 10 s, stop ultrasonic for 5 s), and cooled to room temperature to obtain ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:10, and then the pH value is adjusted to 7 with HCl and NaOH, and magnetically stirred at 25°C for 40min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a s...
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