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Ultrasonic modified whey protein chelated ferrous iron supplementing agent and preparation method thereof

A whey protein, ultrasonic modification technology, applied in the biological field, can solve the problems of unstable structure, low absorption rate, gastrointestinal tract irritation, etc.

Inactive Publication Date: 2021-06-08
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the development experience of iron supplements, whether it is the first generation of iron supplements represented by ferrous sulfate, including inorganic iron salts such as ferrous chloride and ferrous sulfate, or complexed with soluble small molecule organic iron salts The second-generation iron salts represented by iron salts have unstable structures, low absorption rates, and irritating effects on the gastrointestinal tract. Long-term use will also cause adverse side effects such as nausea and diarrhea.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. The preparation method of ultrasonically modified whey protein chelated ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare a whey protein solution with a mass concentration of 40 mg / mL with distilled water, and mix whey protein The solution was ultrasonically modified at an ultrasonic frequency of 20 kHz, an ultrasonic amplitude of 20%, and an ultrasonic time of 5 minutes (every ultrasonic 10 s, stop ultrasonic 5 s), and cooled to room temperature to obtain an ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:1, and then the pH value is adjusted to 6 with HCl and NaOH, and magnetically stirred at 25°C for 20min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a speed ...

Embodiment 2

[0025] 1. The preparation method of ultrasonic modified whey protein ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, and whey protein solution is in Ultrasonic modification was carried out under the conditions of ultrasonic frequency of 20 kHz, ultrasonic amplitude of 30%, and ultrasonic time of 10 min (every ultrasonic 10 s, stop ultrasonic for 5 s), and cooled to room temperature to obtain ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:5, and then the pH value is adjusted to 6 with HCl and NaOH, and magnetically stirred at 25°C for 40min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a ...

Embodiment 3

[0028] 1. The preparation method of ultrasonic modified whey protein ferrous nanoparticles: take whey protein isolate (WPI) as raw material, prepare the whey protein solution that mass concentration is 40mg / mL with distilled water, and whey protein solution is in Ultrasonic modification was performed under the conditions of ultrasonic frequency of 20 kHz, ultrasonic amplitude of 20%, and ultrasonic time of 15 min (every ultrasonic 10 s, stop ultrasonic for 5 s), and cooled to room temperature to obtain ultrasonically modified whey protein solution. FeSO was then added to the whey protein solution 4 , the mass ratio of the two is 1:10, and then the pH value is adjusted to 7 with HCl and NaOH, and magnetically stirred at 25°C for 40min to carry out a sufficient chelation reaction. Then centrifuge at a speed of 3800r / min for 10min to remove insoluble iron and protein and retain the supernatant. Then use a 10kDa ultrafiltration centrifuge tube to centrifuge the supernatant at a s...

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PUM

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Abstract

The invention relates to an ultrasonic modified whey protein chelated ferrous iron supplementing agent and a preparation method thereof, and belongs to the technical field of biology. According to the invention, whey protein isolate and FeSO<4> are taken as raw materials; the whey protein isolate is modified through an ultrasonic technology; the iron chelating capacity of whey protein is taken as an index; ultrasonic modification conditions are optimized; finally, the in-vitro gastrointestinal stability of the whey protein is researched; and the iron supplementing agent which is easy to absorb and high in bioavailability is prepared. The whey protein isolate is subjected to ultrasonic treatment, so that the protein has good biological safety, biocompatibility and biodegradability and ideal functionality of chelating with iron; meanwhile, the problems that the solubility of the whey protein is reduced, the chelating capacity of the whey protein with iron is reduced and the like possibly caused by traditional heat treatment are avoided; the method has the advantages of high efficiency, environmental protection, good iron supplement effect, few side effects and the like. According to the invention, technical support and reference are provided for application of the whey protein chelated iron as the iron supplement.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an ultrasonically modified whey protein isolate chelated ferrous iron and a preparation method thereof. Whey protein isolate is used as a raw material to chelate ferrous ions to prepare ferrous iron with higher iron content. Ultrasonic modification of whey protein chelates iron nanoparticles, and finally prepares an easily absorbed and highly bioavailable iron supplement. Background technique [0002] Iron is one of the trace elements necessary for human growth and development. It plays a variety of physiological roles in the human body, such as participating in the transportation and storage of oxygen, maintaining normal hematopoietic function, and enhancing human immunity. The most common hazard of iron deficiency is anemia, which can cause indigestion, low resistance, slow growth, mental retardation and other conditions in adolescents. Iron deficiency in adults will c...

Claims

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Application Information

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IPC IPC(8): A23L33/165A23L33/19
CPCA23L33/19A23L33/165A23V2002/00A23V2250/1592A23V2250/54252
Inventor 奚春宇孙正伟刘璐刘于嘉李振龙李龙翔
Owner JILIN UNIV
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