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Ultrasonic extraction process for effective components of safflower based on LS-SVM model

An LS-SVM model and technology of active ingredients, applied in solvent extraction, separation methods, solid solvent extraction, etc., can solve problems such as instability of high-latitude nonlinear problems, and achieve the effect of high reliability and small model error

Inactive Publication Date: 2021-06-08
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the prior art deals with the unstable defects of high-latitude nonlinear problems, the purpose of the present invention is to provide a safflower active ingredient ultrasonic extraction process based on the LS-SVM model

Method used

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  • Ultrasonic extraction process for effective components of safflower based on LS-SVM model
  • Ultrasonic extraction process for effective components of safflower based on LS-SVM model
  • Ultrasonic extraction process for effective components of safflower based on LS-SVM model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] HPLC determination of SalB and TanⅡA:

[0024] Chromatographic conditions: chromatographic column is HypersilBDSC18 (4.6mm×300mm, 5μm); mobile phase is acetonitrile (A), 0.1% formic acid water (B), using gradient elution: 0-12min, 10%-22%A; 12- 30min, 22%-30%A; 30-38min, 30%-45%A; 38-50min, 45%-90%A; 50-55min, 90%-90%A; 55-56min, 90%-95 %A; 56-60min, 95%-10%A; flow rate 1mL / min; column temperature 25°C; detection wavelength 280nm; injection volume 10μL.

[0025] Preparation of reference substance solution: Accurately weigh the appropriate amount of HSYA and AHSYB reference substances, place them in 10mL volumetric flasks, add 75% methanol to the mark, shake well, and prepare HSYA1.00mg / mL and AHSYB1.00mg / mL respectively. mL of single-component control substance stock solution. Accurately draw 4.00mL and 0.50mL of the above two stock solutions respectively, place them in the same 10mL measuring bottle, add 75% methanol to dilute to the mark, and shake well to obtain th...

Embodiment 2

[0029] Specificity test: Based on Example 1, accurately draw an appropriate amount of mixed reference substance solution and safflower test solution to measure according to the chromatographic conditions, the peaks of HSYA and AHSYB are completely separated, the peak shape is better, and the resolution is greater than 1.50, see figure 1 , indicating that the method has good specificity.

[0030] Precision test: Accurately draw 0.50mg / mL of HSYA and 0.05mg / mL of AHSYB reference substance solution and repeat the parallel measurement 6 times according to the chromatographic conditions, and the peak area RSD values ​​are 0.69% and 0.76%, indicating that the precision of the instrument is good .

[0031] Stability test: Take the test solution according to the chromatographic conditions and room temperature conditions, and inject samples at 0, 2, 4, 6, and 8 hours respectively, record the chromatographic peak areas of HSYA and AHSYB, and calculate the RSD values ​​​​to be 1.24% and ...

Embodiment 3

[0035] Single factor experiment (based on Example 1):

[0036] The influence of ethanol concentration on extraction rate: Accurately weigh 5 parts of 5g safflower respectively, at liquid-solid ratio 12:1, temperature 55 ℃ and ethanol concentration 50%, 60%, 70%, 80%, 90%, ultrasonic frequency is Under the condition of 40kHz ultrasonic extraction for 40min, the experimental results show that when the ethanol concentration is 80%, the extraction rates of HSYA and AHSYB are the highest, so 70%, 80%, 90% are selected as the three levels of ethanol concentration in the response surface design.

[0037] The influence of ultrasonic time on the extraction rate: Accurately weigh 5 parts of 5g safflower respectively, and extract respectively 20min, 30min, 40min, 50min, 60min, the experimental results show that when the ultrasonic time is 50min, the extraction rates of HSYA and AHSYB are the highest, so 40min, 50min, and 60min are selected as the three levels of ultrasonic time in the re...

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Abstract

The invention discloses an ultrasonic extraction process for effective components of safflower based on an LS-SVM model. The process comprises the following steps: S1, carrying out ultrasonic extraction on safflower effective components HSYA and AHSYB according to a Box-BenhnkenDesign principle; S2, establishing a least square support vector machine model (LS-SVM); S3, optimizing a model parameter kernel parameter g and a penalty factor C, adopting a radial basis function (RBF) as a kernel function, and using Matlab software for editing; S4, inputting the optimal parameter g and the optimal parameter C in the step S3 into the LS-SVM model to obtain a predicted comprehensive evaluation value of the Box-BenhnkenDesign analysis scheme in the step S1; and S5, evaluating the simulation performance of the LS-SVM model by using a mean square error (MSE), wherein the expression of MSE is shown in the specification, m is the number of data set groups, yi is a predicted comprehensive evaluation value of the LS-SVM, and Yi is a real comprehensive evaluation value. The invention relates to the technical field of traditional Chinese medicine extraction. According to the invention, the problem of unstable high-latitude nonlinear problem processing in the prior art is solved.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to an ultrasonic extraction process of active ingredients of safflower based on an LS-SVM model. Background technique [0002] Safflower is a traditional Chinese medicine that has the functions of dispelling blood stasis and stopping bleeding, promoting blood circulation and stimulating menstruation, clearing heart and eliminating troubles. In terms of pharmacology, safflower has the functions of protecting cardiovascular, improving blood circulation, anti-oxidation, protecting brain tissue ischemia, protecting reperfusion injury, enhancing hypoxia resistance and improving renal function. There are two main types of active ingredients in safflower, fat-soluble safflower ketone compounds and water-soluble phenolic acid compounds, among which there are as many as dozens of active ingredients that play a positive role. [0003] Support Vector Machine (S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D11/02G16C10/00
CPCB01D11/0265G16C10/00
Inventor 应雨棋虞立金伟锋
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY