Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of α-santalene synthase, gene and application

A technology of santalene and synthetase, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., and can solve the problem of low product specificity

Active Publication Date: 2022-08-05
HANGZHOU NORMAL UNIVERSITY
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on santalene synthase. For example, santalene synthase was cloned from Santalum album, S. austrocaledonicum and S. spicatum respectively, but the product specificity is not high, except for the generation of α / In addition to β-santalene, there are also its isomers epi-β-santalene, bergamotene and other products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of α-santalene synthase, gene and application
  • A kind of α-santalene synthase, gene and application
  • A kind of α-santalene synthase, gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of α-santalene synthase, gene, vector and engineered bacteria derived from Turmeric

[0020] 1. Homology analysis of candidate CwSS genes

[0021] The tuberous root, tender leaves, buds and stem tissues were collected from both sides of Feiyun River in Ruian, Wenzhou, and the transcriptome was sequenced by Shanghai Meiji Biotechnology Company. Bioinformatics analysis of the obtained sequences revealed a candidate sesquiterpene synthase gene ( That is, the α-santalene synthase of the present application, CwSS), its deduced amino acid sequence was subjected to Blastp analysis at NCBI, and it was found that it had the greatest similarity with the bisabolene synthase (Accession Number: D2YZP9) derived from Zingiber officinale. source, the sequence identity reaches 86%; further sequence alignment with other sources of sesquiterpene synthase shows that CwSS has conserved domains, such as DDxxD, RXR, NSE / DTE, RRX 8 W( figure 1 ).

[0022] Phylogenetic...

Embodiment 2

[0028] Example 2: Analysis of Catalytic Properties of Recombinase CwSS

[0029] The CwSS recombinase solution (concentration is 50mg / L, volume is 10μL) prepared by the method of Example 1 is used as catalyst, pH 7.0 Tris-HCl buffer solution, 2μg FPP (farnesyl pyrophosphate), 500mM MgCl are added. 2 20 μL of the solution, 2 μL of 500 mM DTT, and 100 μL of dilute glycerol (1,2,3-propanetriol) constitute 1 ml of the reaction system, respectively, and fully react at 30 °C for 120 min. After the reaction was completed, the extraction head was taken out and injected into a gas chromatograph to conduct qualitative analysis of the product. Chromatographic conditions: GC-2010 Shimadzu gas chromatograph; chromatographic column HP-5; carrier gas: N 2 , the purge flow is 3mL / min, no split; the initial temperature of the column oven is 40°C, and it is kept for 2 minutes, and then it is heated to 220°C at a rate of 7°C / min, and it is kept for 5 minutes; the inlet temperature is 250°C; The...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an α-santalene synthase, a gene and an application. The α-santalene synthase is derived from Curcuma wenyujin Y.H.Chen et C.Ling, and the amino acid sequence is shown in SEQ ID NO.2. The present invention verifies the function of the α-santalene synthase derived from turmeric, and the invention and application of a method for synthesizing α-santalene in Escherichia coli. It was found that the α-santalene synthase derived from turmeric can catalyze FPP to generate a large amount of α-santalene, as well as a small amount of β-santalene and α-farnesene in the prokaryotic expression system, and the α-santalene was calculated according to the peak area. The content of stilbene has reached more than 90%, so that the catalyst can be prepared rapidly and in large quantities in Escherichia coli, and is applied to the synthesis of α-santalene, and further can be applied to the synthesis of α-santalol, which has important advantages. significance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an α-santalene synthase, a gene and an application. Background technique [0002] α-Santalene (α-santalene), the structural formula is shown in the following formula I, is a sesquiterpenoid volatile component extracted from sandalwood tree, which can generate α-santalene under the catalysis of cytochrome oxidase P450, α- Sandalwood and alpha-santalol are the main components of sandalwood essential oil. Sandalwood essential oil has low toxicity and no mutagenicity, and is currently recognized as a safe food additive. Sandalwood essential oil is not only used in fragrances and cosmetics, but also has sedative, anti-inflammatory, analgesic, antibacterial, antiviral, antioxidant, and antitumor effects. It has certain curative effect on skin diseases, bronchitis, mucositis, depression and insomnia. Although sandalwood essential oil has a very important role and its market value is const...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/70C12N1/21C12P15/00C12R1/19
CPCC12N9/88C12N15/70C12P15/00C12Y402/03082
Inventor 谢恬殷晓浦谌容卫秋慧刘雨恒魏昕
Owner HANGZHOU NORMAL UNIVERSITY