Process for preparing injection liquid of brain protein hydrolyzate

A technology of cerebroprotein hydrolyzate and production method, which is applied in the field of production of cerebroprotein hydrolyzate injection, can solve the problems that are not conducive to the control of drug quality, damage to human health, and low production efficiency, and achieve shortened production time and low production efficiency , the effect of easy operation

Inactive Publication Date: 2003-12-03
HAINAN STAR PHARM CO LTD
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  • Abstract
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Problems solved by technology

[0004] As we all know, acetone and ether have extremely low boiling points, are flammable and explosive, and are toxic organic chemicals. Ether is also used as an anesthetic. A large amount of acetone and ether is used to precipitate brain protein to make brain powder. This step is very dangerous. , must be produced in an explosion-proof workshop, which is troublesome to operate, pollutes the environment, and damages human health
[0005] (2), equipment investment is large, production efficiency is low, and production cycle is long
Using the steps of vacuum concentration and centrifugation, the production efficiency is low, which is not conducive to the control of drug quality

Method used

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Embodiment Construction

[0012] 1. 100 kg of fresh pig brain, remove surface mucous membranes and blood stains, etc., add 200 L of distilled water (hereinafter referred to as water) while homogenizing, filter the homogenate with double-layer gauze; add 2.16 kg of pepsin into the filtrate after adding aqueous solution, Adjust the pH to 1.5 with 0.5N hydrochloric acid, hydrolyze in a water bath at 44°C for 8 hours, then adjust the pH to 7.2 with 2N sodium hydroxide, take 1.25 kg of trypsin and activate it with 0.02N calcium chloride at 4°C for 30 minutes, After the addition, continue to adjust the pH to 7.6 with 2N sodium hydroxide, hydrolyze in a water bath at 44°C for 6 hours to obtain a hydrolyzate, adjust the pH to 3.0 with 0.5 hydrochloric acid, and let stand overnight at -10°C; filter once with double gauze, and then Add 150L of water to make the total volume about 450L, adjust the pH value to neutral with 2N sodium hydroxide, filter with a microporous membrane with a pore size of 0.6um, and then p...

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Abstract

A process for preparing the liquid injection of brain protein hydrolyzate includes mixing water with pig's brain, homogenizing, filtering, hydrolyzing with pepsin and then pancreatin, regulating pH value, laying aside, filter, adding water to regulate pH value to be neutral, filtering with millipore filter, superfiltering with ultrafiltering membrane, heating, cooling, and ultrafiltering.

Description

technical field [0001] The present invention belongs to the production method of biochemical medicine. Background technique [0002] Existing technology: remove the mucous membrane and blood stains on the surface of fresh pig brain, homogenate, add 5 times the volume of acetone to precipitate, add the same amount of ether to wash and soak for 1 hour after the precipitation is separated, then filter and vacuum dry to make brain powder; After the powder is dissolved in water, it is hydrolyzed with pepsin and pancreatin in turn, the hydrolyzate is centrifuged to take the supernatant, heated to boil and cooled rapidly, and then the hydrolyzate is obtained by plate and frame filtration, ultrafiltration and reduced pressure concentration; the hydrolyzate is batched Go up Sephadex G-25 gel chromatography column chromatography, the active peak collection liquid is made fine liquid through decompression concentration, 0.45 microporous filtration; The drug standard (No. WS1-XG-25-200...

Claims

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Application Information

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IPC IPC(8): A61K35/30A61K38/01
Inventor 张素戴毅赵守田
Owner HAINAN STAR PHARM CO LTD
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