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Method for detecting DHA in cosmetics, and application thereof

A determination method and cosmetic technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low accuracy of results, cumbersome DHA detection method, etc., and achieve the effects of high sensitivity, suppression of interference of impurities, and high reproducibility

Pending Publication Date: 2021-06-11
上海晓创检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention solves the defects of cumbersome DHA detection method in cosmetics and low accuracy of results in the prior art by providing a method for determining DHA in cosmetics, and realizes an efficient and accurate method for detecting DHA in cosmetics

Method used

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  • Method for detecting DHA in cosmetics, and application thereof
  • Method for detecting DHA in cosmetics, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The first aspect of this embodiment provides a method for the determination of DHA in cosmetics, the specific steps include:

[0058] S1. Pretreating the sample to obtain the solution to be tested;

[0059] S2. Prepare DHA standard solution and mobile phase;

[0060] S3. Use a high-performance liquid chromatograph to detect the liquid to be tested and the DHA standard solution, and the high-performance liquid chromatograph includes a sample injection module, a chromatographic column and a detector.

[0061] The pretreatment step in the S1 step includes: weighing 1g of the sample into a 50mL stoppered colorimetric tube, adding 10mL of the extract, shaking, ultrasonically extracting for 15min, centrifuging and standing still, using a 0.45um filter membrane to filter the supernatant Filter and collect the filtrate, which is the solution to be tested.

[0062] The extract is an 80% acetonitrile aqueous solution by volume fraction.

[0063] The concentration gradient of p...

Embodiment 2

[0072] The first aspect of this embodiment provides a method for the determination of DHA in cosmetics, the specific steps include:

[0073] S1. Pretreating the sample to obtain the solution to be tested;

[0074] S2. Prepare DHA standard solution and mobile phase;

[0075] S3. Use a high-performance liquid chromatograph to detect the liquid to be tested and the DHA standard solution, and the high-performance liquid chromatograph includes a sample injection module, a chromatographic column and a detector.

[0076] The pretreatment step in the S1 step includes: weighing 1g of the sample into a 50mL stoppered colorimetric tube, adding 8mL of the extract, shaking, ultrasonically extracting for 15min, leaving it to stand after centrifugation, and using a 0.45um filter membrane to decontaminate the supernatant. Filter and collect the filtrate, which is the solution to be tested.

[0077] The extract is an 80% acetonitrile aqueous solution by volume fraction.

[0078] The concent...

Embodiment 3

[0087] The first aspect of this embodiment provides a method for the determination of DHA in cosmetics, the specific steps include:

[0088] S1. Pretreating the sample to obtain the solution to be tested;

[0089] S2. Prepare DHA standard solution and mobile phase;

[0090] S3. Use a high-performance liquid chromatograph to detect the liquid to be tested and the DHA standard solution, and the high-performance liquid chromatograph includes a sample injection module, a chromatographic column and a detector.

[0091] The pretreatment step in the S1 step includes: weighing 1g of the sample into a 50mL stoppered colorimetric tube, adding 10mL of the extract, shaking, ultrasonically extracting for 15min, centrifuging and standing still, using a 0.45um filter membrane to filter the supernatant Filter and collect the filtrate, which is the solution to be tested.

[0092] The extract is an 80% acetonitrile aqueous solution by volume fraction.

[0093] The concentration gradient of p...

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Abstract

The invention relates to the technical field of cosmetic detection, in particular to a method for detecting DHA in cosmetics, and application thereof. The method comprises the following specific steps: S1, pretreating a sample to obtain a to-be-detected solution; S2, preparing a DHA standard solution and a mobile phase; and S3, detecting the to-be-detected solution and the DHA standard solution by using a high performance liquid chromatograph, wherein the high performance liquid chromatograph comprises a sample introduction module, a chromatographic column and a detector. The provided method for detecting DHA in cosmetics has high reproducibility and sensitivity, the detection limit is low, the effect of accurately and effectively detecting DHA in cosmetics can be achieved, and interference of impurities in complex formulas of cosmetics is inhibited; and meanwhile, the method is simple and easy to implement, high in practicability and wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of cosmetic detection, in particular to a method for determining DHA in cosmetics and an application thereof. Background technique [0002] Dihydroxyacetone (Dihydroxyacetone) referred to as DHA, is a polyhydroxy ketose, is the simplest ketose, the appearance is a white powder crystal, soluble in water, ethanol, ether and acetone and other organic solvents. As a naturally occurring ketose sugar in nature, DHA is biodegradable, edible and non-toxic to the human body and the environment. It can be used in many industries such as cosmetics, medicine and food. Specifically, in cosmetics, the main function of DHA is sunscreen and reducing agent. Adding it to cosmetics can prevent excessive evaporation of skin moisture, and play the role of moisturizing, sunscreen and UV radiation protection. In addition, the ketone functional group in DHA can react with the amino acid and amino group of skin keratin to form a br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/74G01N30/88
CPCG01N30/02G01N30/04G01N30/06G01N30/14G01N30/34G01N30/74G01N30/88G01N2030/042G01N2030/146G01N2030/884
Inventor 李蕊陈阳明
Owner 上海晓创检测技术有限公司
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