Construction method of algae-bacteria symbiotic system for removing ARGs in water body

A technology of algae-bacteria symbiosis and construction method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of quick and simple operation and low cost
CN112961880APending Publication Date: 2021-06-15SHANGHAI INST OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI INST OF TECH
Publication Date
2021-06-15

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Abstract

The invention discloses a construction method of an algae-bacteria symbiotic system for removing antibiotic resistance genes in a water body. The construction method comprises the following steps: S1, inoculating common chlorella vulgaris cells and bacillus licheniformis into a sterilized BG11-LB culture medium; S2, culturing the mixed culture medium inoculated with the chlorella vulgaris cells and the bacillus licheniformis cells in a sterile room with constant temperature and constant illumination; S3, preparing artificial sewage containing antibiotic resistance gene plasmids; S4, mixing the mixed culture medium in the step S2 with the artificial sewage in the step S3 to form the algae-bacteria symbiotic system; and S5, taking 1-2mL of the algae-bacteria mixed solution in the step S4, performing cell perforation on the taken algae-bacteria mixed solution by adopting a cell electroporation method, cooling, placing the algae-bacteria mixed solution in the algae-bacteria symbiotic system in the step S4 again, and continuously culturing in the sterile room with constant temperature and constant illumination. The algae-bacteria symbiotic system provided by the invention has a good removal effect on the ARGs such as sul1, sul2, tetM, tetQ, tetW and the like in the natural water body.
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Description

technical field

[0001] The invention belongs to the technical field of antibiotic resistance gene pollution treatment, and in particular relates to a method for constructing an algae-bacteria symbiosis system for removing antibiotic resistance genes in water bodies. Background technique

[0002] Antibiotic resistance genes (ARGs) pollution has become a water pollution problem that has attracted much attention in the world today. The extensive use of antibiotics in medical treatment, animal husbandry, aquaculture and other fields has caused the accumulation of ARGs pollution in water bodies in the environment. According to reports, ARGs have been detected to varying degrees in many water bodies in my country, among which sulfonamides and tetracycline ARGs are ubiquitous and at high concentrations. Water is an important medium for the release and diffusion of ARGs. ARGs can enter the human food chain through the water cycle, posing a serious threat to the ecological environme...

Claims

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