Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method of algae-bacteria symbiotic system for removing ARGs in water body

A technology of algae-bacteria symbiosis and construction method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of quick and simple operation and low cost

Pending Publication Date: 2021-06-15
SHANGHAI INST OF TECH
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the deficiencies of the existing water body antibiotic resistance gene pollutant removal technology, the invention provides a method for constructing an algal symbiosis system that removes antibiotic resistance genes in water. ARGs such as sulfonamide antibiotic resistance genes (sul1, sul2) and tetracycline antibiotic resistance genes (tetM, tetQ, tetW) in water have good removal effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of algae-bacteria symbiotic system for removing ARGs in water body
  • Construction method of algae-bacteria symbiotic system for removing ARGs in water body
  • Construction method of algae-bacteria symbiotic system for removing ARGs in water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The concentration of algae cells was 1×10 8 cell / mL Chlorella vulgaris (purchased from the Freshwater Algae Species Bank of the Chinese Academy of Sciences, algae cell number: FACHB-8) 5mL, the concentration of the bacterial cells was 1×10 6 CFU / mL of Bacillus licheniformis (purchased from the Culture Collection Center of the Chinese Academy of Sciences, algae cell number: 1.7461) 5mL; centrifuge the two separately (8000g / min, 3min), discard the supernatant, and separate the two precipitates Inoculate in 150mL of sterilized BG11-LB medium and culture in a sterile room with constant temperature and constant light, set the temperature at 28°C, and set the light intensity at 120μmol / m 2 / s, wherein, BG11 medium, LB medium volume ratio is 1:1;

[0063] Add 0.011-65.2 μg of sul1, 0.010-58.6 μg of sul2, 0.010-58.6 μg of tetM, 0.011-64.2 μg of tetQ, and 0.010-58.6 μg of tetW to the artificial sewage to configure a plasmid concentration of 2.09× 10 5 -1.26×10 9 copies / mL of...

Embodiment 2

[0073] Reduction of Antibiotic Resistance Genes in Real River Wastewater Using Algae-bacteria Symbiosis System

[0074] 200-500mL of river sewage was collected, and the abundance of ARGs in the river water was found to be sul1: 1.5×10 5 copies / mL, sul2: 0.4×10 5 copies / mL, tetM: 1.2×10 5 copies / mL, tetQ: 2.35×10 5 copies / mL, tetW: 0.78×10 5 copies / mL,

[0075] Mix 150mL of BG11-LB medium inoculated with Chlorella and Bacillus licheniformis with the collected river water, and the initial dosage concentration of the algal symbiont is 2.5×10 6 cell / mL, the culture temperature is 28±2℃, the light-dark ratio is 12h:12h, and the light intensity is 120μmol / m 2 Cultivate under the / s culture condition for 5 days to obtain algae-bacteria symbiosis (algae-bacteria mixed solution);

[0076] After the electroporation treatment, the cells were continued to be cultured in the collected river sewage. The relative abundances of sul1, sul2, tetM, tetQ, and tetW in the water were: 0.3×10 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a construction method of an algae-bacteria symbiotic system for removing antibiotic resistance genes in a water body. The construction method comprises the following steps: S1, inoculating common chlorella vulgaris cells and bacillus licheniformis into a sterilized BG11-LB culture medium; S2, culturing the mixed culture medium inoculated with the chlorella vulgaris cells and the bacillus licheniformis cells in a sterile room with constant temperature and constant illumination; S3, preparing artificial sewage containing antibiotic resistance gene plasmids; S4, mixing the mixed culture medium in the step S2 with the artificial sewage in the step S3 to form the algae-bacteria symbiotic system; and S5, taking 1-2mL of the algae-bacteria mixed solution in the step S4, performing cell perforation on the taken algae-bacteria mixed solution by adopting a cell electroporation method, cooling, placing the algae-bacteria mixed solution in the algae-bacteria symbiotic system in the step S4 again, and continuously culturing in the sterile room with constant temperature and constant illumination. The algae-bacteria symbiotic system provided by the invention has a good removal effect on the ARGs such as sul1, sul2, tetM, tetQ, tetW and the like in the natural water body.

Description

technical field [0001] The invention belongs to the technical field of antibiotic resistance gene pollution treatment, and in particular relates to a method for constructing an algae-bacteria symbiosis system for removing antibiotic resistance genes in water bodies. Background technique [0002] Antibiotic resistance genes (ARGs) pollution has become a water pollution problem that has attracted much attention in the world today. The extensive use of antibiotics in medical treatment, animal husbandry, aquaculture and other fields has caused the accumulation of ARGs pollution in water bodies in the environment. According to reports, ARGs have been detected to varying degrees in many water bodies in my country, among which sulfonamides and tetracycline ARGs are ubiquitous and at high concentrations. Water is an important medium for the release and diffusion of ARGs. ARGs can enter the human food chain through the water cycle, posing a serious threat to the ecological environme...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/75C12N15/65C12R1/10C12R1/89
CPCC12N15/8206C12N15/75C12N15/65
Inventor 吉喜燕侯梅芳吴世超唐赟超叶璟
Owner SHANGHAI INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com