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Method for applying porous Co3O4 peroxidase to rapid colorimetric detection of glyphosate

A technology of peroxidase and detection method, which is applied in the field of organophosphorus pesticide residue detection, can solve problems such as not being used, and achieve the effects of simple and rapid operation, good selectivity and high detection sensitivity

Pending Publication Date: 2021-06-15
贵阳海关综合技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, porous Co 3 o 4 peroxidase (porous Co 3 o 4 Nanozyme) activity has not been applied to glyphosate detection

Method used

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  • Method for applying porous Co3O4 peroxidase to rapid colorimetric detection of glyphosate
  • Method for applying porous Co3O4 peroxidase to rapid colorimetric detection of glyphosate
  • Method for applying porous Co3O4 peroxidase to rapid colorimetric detection of glyphosate

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Experimental program
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Effect test

Embodiment 1

[0026] A kind of porous Co3O4 peroxidase is applied to the rapid colorimetric detection method of glyphosate, comprising the following steps:

[0027] (1) Prepare a detection system with known glyphosate concentration: take 11 1.5mL graduated centrifuge tubes, add 10uL glyphosate standard solution respectively (the glyphosate content in the entire detection system is maintained at 5-800ug / L) and 40uL of Co at a concentration of 1ug / mL 3 o 4 Solution, mix well and incubate at 30°C for 10min, then add 415uL NaAc buffer solution with a pH of 6.0 and a concentration of 5mM, and finally add 35μL of 8mM H 2 o 2 Mix the solution with the substrate of 1.5 mM TMB so that the total volume of the entire sensing solution reaches 500 μL of the mixed solution, mix well and incubate at 30° C. for 10 min, and the incubated solution is to be tested.

[0028] (2) Take another test solution prepared according to step (1), add 10 μL of ultrapure water to replace the glyphosate standard solutio...

Embodiment 2

[0036] Be actual sample with tomato, cucumber and cabbage, repeat embodiment 1 step, see the following table:

[0037]

[0038] Actual sample detection effect verification: the method of the present invention is used to determine tomato, cucumber and cabbage as actual samples, and the recovery rate obtained is between 86.4% and 95.2%.

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Abstract

The invention discloses a method for applying porous Co3O4 peroxidase to rapid colorimetric detection of glyphosate. The method comprises the following steps: (1) uniformly mixing a sample containing glyphosate with a porous Co3O4 solution with the concentration of 1 [mu]g / mL, and incubating at 30 DEG C for 10 minutes; and (2) adding a NaAc buffer solution with the pH value of 6.0 and the concentration of 5 mM, H2O2 with the concentration of 8 mM and a TMB substrate solution with the concentration of 1.5 mM into the mixed solution, carrying out chromogenic reaction for 10 minutes, and determining the content of glyphosate by measuring the change of a light absorption value of the mixed solution at 650 nm. The lowest detection limit of glyphosate is 2.37 [mu]g / L, and the method has the advantages of being good in detection specificity, capable of being distinguished by naked eyes, easy and convenient to operate and the like, can achieve rapid detection of glyphosate pesticide residues without depending on large instruments, and can be widely applied to detection of organophosphorus pesticides in agricultural products.

Description

technical field [0001] The invention belongs to the field of detection of organophosphorus pesticide residues in agricultural products, and in particular relates to a method for detecting the characteristic absorption peak change at 650 nm of a detection system by using a colorimetric method in an aqueous buffer solution system, and visually determining glyphosate pesticides. Background technique [0002] Glyphosate is a broad-spectrum organophosphorus herbicide, which causes plant death by inhibiting the activity of 5-enolpyruvylshikimate-3-phosphatase in an important plant metabolic pathway. In the past few decades, Due to its high herbicidal activity, it is widely used in vegetation control in agriculture. Although the assessment agency lists it as a low-hazard compound, studies have shown that glyphosate can affect cell regulation, cause chronic kidney disease, and even cause cancer. At present, the abuse of glyphosate has caused the pollution of soil, water and agricul...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 黄小焕周程寅刘松徐佳吴远根
Owner 贵阳海关综合技术中心
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