Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacterial strain for efficiently degrading anthraquinone dyes and method for improving decolorization efficiency by using traditional Chinese medicine waste residues

A technology of anthraquinones and decolorization rate, which is applied in the field of white rot fungal strains and traditional Chinese medicine extraction waste residue to improve the decolorization efficiency of dye wastewater, and can solve the problems of large wastewater discharge, environmental pressure, resource waste, and environmental pollution.

Active Publication Date: 2021-06-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

White-rot fungi have abundant degradative enzymes. Gardenia waste residue can be used as an additional culture ingredient to stimulate the expression of various extracellular enzymes, thereby further improving the degradation effect of dyes. At present, no white-rot fungi have been used Research Report on Improvement of Dye Degradation Effect by Waste Residue of Traditional Chinese Medicine
[0006] At present, the discharge of industrial dye wastewater is large, causing serious environmental pollution, and its treatment is still very challenging. At the same time, a large amount of traditional Chinese medicine waste residues also cause huge environmental pressure and waste of resources.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacterial strain for efficiently degrading anthraquinone dyes and method for improving decolorization efficiency by using traditional Chinese medicine waste residues
  • Bacterial strain for efficiently degrading anthraquinone dyes and method for improving decolorization efficiency by using traditional Chinese medicine waste residues
  • Bacterial strain for efficiently degrading anthraquinone dyes and method for improving decolorization efficiency by using traditional Chinese medicine waste residues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Screening and identification of strains degrading anthraquinone dyes

[0046] This bacterial strain is isolated from the soil microorganism of Nanjing University of Traditional Chinese Medicine Medicinal Botanical Garden, and solid medium is potato dextrose agar medium (20% of potato juice, glucose 2%, agar powder 2%), and liquid medium is (20% of potato juice, glucose 2%), after being sterilized at 115° C. for 30 minutes, the mycelium is radial on the solid plate, the mycelium is luxuriant, white, and does not produce spores and pigments. Through 18sRNA homology identification and phylogenetic analysis, the strain is Antrodia malicola, which is named P5. Depository unit: China Center for Type Culture Collection, preservation number CCTCC M 2020865,

Embodiment 2

[0048] Decolorization efficiency of P5 to different dyes. Inoculate P5 on a PDA plate, culture at 28°C for 2 days, scrape off the fresh mycelium with sterile water into 100ml PDB medium, culture at 28°C, 160rpm for 2 days as seed liquid, transfer to 100ml at a ratio of 5% Fresh PDB medium was cultured for 2 days, and 100 mg of 5 different common synthetic dyes were added, including reactive blue 4, malachite green, methyl orange, direct black 19, and acid red. Take the initial absorbance as A 0 , take samples every 24h, record the corresponding absorbance as A1, and use the formula: decolorization rate=A 0 -A 1 / A 0 Calculate the decolorization efficiency of P5 to different dyes. For the decolorization efficiency of P5 to 5 different dyes, see figure 1 . Among them, the decolorization efficiency of reactive blue 4 is the highest, and can reach 95% after 24 hours of decolorization. The experimental results show that P5 has a wide range of dye decolorization capabilities...

Embodiment 3

[0050] Add different concentrations of dye reactive blue 4 to PDB medium to investigate the decolorization ability of P5 to different concentrations of reactive blue 4. In the PDB medium, add the reactive blue 4 dyestuff that final concentration is respectively 1000mg / L, 1500mg / L, 2000mg / L, 2500mg / L respectively, figure 2 The results showed that P5 had the highest decolorization efficiency at 1000 mg / L, and the decolorization efficiency of P5 decreased with the increase of dye concentration. However, P5 has a high tolerance to reactive blue 4, and can still degrade the dye at a high concentration of 2500mg / L.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
decolorization rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a white rot fungus pellicle P5 strain for efficiently degrading anthraquinone dye reactive blue 4. P5 adopts a potato glucose culture medium, and under the conditions of 160rpm and 30 DEG C, the 24-hour decolorization rate of reactive blue 4 dye with the concentration of 1000mg / L is 95%. The highest tolerance concentration of the P5 to reactive blue 4 is 2500mg / L, and the P5 can adapt to dye wastewater conditions with a wide pH range (4-9) and a salt ion concentration of 10%. Meanwhile, the degradation efficiency of the P5 on the dye reactive blue 4 is remarkably improved by utilizing the traditional Chinese medicine gardenia waste residues, the degradation efficiency of the P5 on the reactive blue 4 is remarkably improved in a potato glucose liquid culture medium added with (1%, m / v) gardenia waste residues, and the decolorization rate can reach 92% within 12 h. Besides, after the gardenia waste residues are added, the P5 can obviously reduce the concentration of degradation metabolites of the reactive blue 4 and the acute toxicity of degradation wastewater.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a white-rot fungus strain for efficiently degrading anthraquinone dyes, and a method for improving the decolorization efficiency of dye wastewater by using traditional Chinese medicine to extract waste residue. Background technique [0002] At present, there are about 10,000 kinds of synthetic dyes used in the world, with an annual production capacity of 700,000 tons, which are widely used in many industrial production fields such as textiles, leather, detergents, pharmaceuticals and cosmetics. Demand is growing even more (Kathersan et al., 2018). However, at present, 70% of dye wastewater is directly discharged into the natural environment without treatment, which has caused huge pressure on human ecological environment and health. Therefore, it is urgent to treat industrial dye wastewater, thereby reducing the impact of dye industry on the human environment. pressure (Varjan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C02F101/30C12R1/645
CPCC12N1/14C02F3/347C02F2101/308
Inventor 袁天杰陈乐天张淑仪陈奕妃张然张芳
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE