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Extraction process of andrographolide

A technology of andrographolide and extraction process, which is applied in the direction of organic chemistry, can solve the problems of inability to industrialize production, low extraction of active ingredients, time-consuming extraction process, etc., and achieve easy industrial production, increase the yield of active ingredients, and improve the purity Effect

Pending Publication Date: 2021-06-29
JIANGXI NEW CENTURY MINXING ANIMAL HEALTH PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, high-purity crystals of andrographolide monomer compounds have not been prepared in actual production. The extraction of andrographolide in the prior art has low active ingredients, long extraction time, time-consuming and complicated extraction process, low purity, and cannot be industrialized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of extraction process of andrographolide, comprising:

[0027] Cleanly select 20g of the traditional Chinese medicine Andrographis paniculata, add 200mL of ethanol solution with a volume fraction of 90% after drying and crushing, and extract twice at 60°C for 3 hours each time; add 1g of 80-110 mesh activated carbon to the obtained extract pH = 7, decolorize at 25°C for 30min, adjust the pH of the mixture to 4 with HCl or NaOH, heat to 45°C, add 0.008g of snail digestive enzyme and 0.008g of pectinase to hydrolyze for 8min, filter and collect the filtrate; The filtrate is concentrated under reduced pressure until the ethanol solvent concentration in the filtrate is lower than 25%, and the concentrated solution is taken to flow through the AB-8 resin column for exchange and adsorption. 75%, 85% ethanol solution for gradient elution, take the ethanol eluate with a volume fraction of 75%; concentrate the eluate and pass it through a membrane (500 equivalents), disso...

Embodiment 2

[0030] A kind of extraction process of andrographolide, comprising:

[0031] Cleanly select 20g of the traditional Chinese medicine Andrographis paniculata, add 200mL of ethanol solution with a volume fraction of 85% after drying and crushing, and extract twice at 60°C for 3 hours each time; add 1g of 80-110 mesh activated carbon to the obtained extract pH = 7, decolorize at 25°C for 25min, adjust the pH of the mixture to 4 with HCl or NaOH, heat to 45°C, add 0.008g of snail digestive enzyme and 0.008g of pectinase to hydrolyze for 6min, filter and collect the filtrate; Concentrate the filtrate under reduced pressure until the concentration of ethanol solvent in the filtrate is lower than 25%, take the concentrated solution and flow through the D101 resin column for exchange and adsorption, the flow rate is 2.8BV / h, and then water, the volume fraction is 55%, 65%, 75% , 85% ethanol solution for gradient elution, get the ethanol eluent of 75% volume fraction; the eluent is conc...

Embodiment 3

[0034] A kind of extraction process of andrographolide, comprising:

[0035] Cleanly select 20g of the traditional Chinese medicine Andrographis paniculata, add 200mL of ethanol solution with a volume fraction of 95% after drying and crushing, and extract twice at 60°C for 3 hours each time; add 1.1g of 80-110 mesh activated carbon to the obtained extract Decolorize at pH=7 and 25°C for 35min, adjust the pH of the mixture to 4 with HCl or NaOH, heat to 45°C, add 0.009g of snail digestive enzyme and 0.009g of pectinase to hydrolyze for 10min, and collect the filtrate by filtration; The obtained filtrate is concentrated under reduced pressure until the ethanol solvent concentration in the filtrate is lower than 25%, and the concentrated solution is taken to flow through the AB-8 resin column for exchange and adsorption at a flow rate of 5.6BV / h, and then water with a volume fraction of 55%, 65% , 75%, 85% ethanol solution for gradient elution, take ethanol eluent with a volume f...

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Abstract

The invention relates to an extraction process of andrographolide, which comprises the following steps: cleaning, drying and crushing a traditional Chinese medicine andrographis paniculata, adding an ethanol solution with the weight volume fraction of 85-95%, which is 10 times that of the traditional Chinese medicine andrographis paniculata, heating and extracting, decolorizing an extracting solution by using activated carbon, performing enzymolysis, filtering, performing vacuum concentration, and carrying out gradient elution by using ethanol solutions with different concentrations after exchanging and adsorbing by using a weakly polar or non-polar macroporous resin column, and finally passing the eluent through a membrane and recrystallizing to obtain the high-purity andrographolide crystal. The extraction process provided by the invention ensures the activity of the andrographolide and improves the purity of the obtained andrographolide, and has the advantages of low cost, simplicity in operation, mild reaction conditions and easiness in industrial production.

Description

technical field [0001] The invention belongs to the technical field of medicine and veterinary medicine, in particular to the technical field of extraction of traditional Chinese medicine, and specifically relates to an extraction process of andrographolide. Background technique [0002] Andrographis paniculata, the name of traditional Chinese medicine. Also known as spring lotus and autumn willow, happy at first sight, elemi, bitter gall grass, golden vanilla, golden ear hook, Indian grass, bitter grass and so on. Medicinal plant, bitter in taste, cold in nature. It has the effects of clearing away heat and detoxification, reducing inflammation, reducing swelling and relieving pain. Commonly used for cold and fever, sore throat, aphtha of the mouth and tongue, cough and cough, diarrhea and dysentery, hot stranguria and astringent pain, carbuncle sores, snake bites. The main active ingredients of Andrographis paniculata are diterpene lactone compounds: andrographolide is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/60
CPCC07D307/60
Inventor 王赞文郭祥义贺智宪刘高艺单亚静
Owner JIANGXI NEW CENTURY MINXING ANIMAL HEALTH PROD CO LTD
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