Metal-catechin composite nano-material as well as preparation method and application thereof

A composite nanomaterial and catechin technology, which is applied in the field of metal-catechin composite nanomaterials and the preparation thereof, can solve the problems of complicated steps, difficult control of catechin release, etc., and achieves low cost, short cycle and stability. boosted effect

Active Publication Date: 2021-06-29
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation method of this chitosan-coated catechin is complicated, relies on toxic substances such as TPP, and the catechin in the obtained folic acid-chitosan-epigallocatechin gallate nanoparticles uncontrollable release

Method used

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  • Metal-catechin composite nano-material as well as preparation method and application thereof
  • Metal-catechin composite nano-material as well as preparation method and application thereof
  • Metal-catechin composite nano-material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for preparing metal-catechin composite nanomaterials, the specific steps are as follows:

[0052] (1) 3mmol (+)-catechin was dissolved in 50mL water to obtain a 60mM (+)-catechin solution; 1mmol ferric chloride hexahydrate was dissolved in 50mL water to obtain a 20mM ferric chloride hexahydrate solution;

[0053] (2) Mix the (+)-catechin solution in step (1) and the ferric chloride hexahydrate solution, and stir for 1 hour at 25° C. to produce a dark brown precipitate;

[0054] (3) Wash the precipitate in step (2) with deionized water and alcohol, centrifuge and freeze-dry to obtain spherical nanoparticles, that is, metal-catechin composite nanomaterials.

[0055] The metal-catechin composite nanomaterial prepared in this embodiment is observed by a scanning electron microscope for its morphology characteristics, and its scanning electron microscope photo is as follows: figure 1 shown. Depend on figure 1 It can be seen that the shape of the metal-catechin co...

Embodiment 2

[0061] A method for preparing metal-catechin composite nanomaterials, the specific steps are as follows:

[0062] (1) 3mmol (+)-catechin was dissolved in 50mL water to obtain a 60mM (+)-catechin solution; 1mmol ferric chloride hexahydrate was dissolved in 50mL water to obtain a 20mM ferric nitrate solution;

[0063] (2) Mix the (+)-catechin solution and ferric nitrate solution in step (1), and stir for 2 hours at 25° C. to produce a dark brown precipitate;

[0064] (3) Wash the precipitate in step (2) with deionized water and alcohol, centrifuge and freeze-dry to obtain spherical nanoparticles, that is, metal-catechin composite nanomaterials;

[0065] Applying the metal-catechin composite nanomaterial prepared above to inhibit the adipogenic differentiation of human adipose-derived mesenchymal stem cells, the steps are as follows:

[0066] (1) Weigh 10 mg of metal-catechin composite nanomaterials, disperse them in 1 mL of phosphate buffered saline (PBS) after sterilization, t...

Embodiment 3

[0070] A method for preparing metal-catechin composite nanomaterials, the specific steps are as follows:

[0071] (1) Dissolve 1.5mmol (+)-catechin in 50mL water to obtain a 30mM (+)-catechin solution; dissolve 0.2mmol ferric nitrate in 20mL water to obtain a 10mM ferric nitrate solution;

[0072] (2) Mix the (+)-catechin solution and ferric nitrate solution in step (1), and stir for 3 hours at 25° C. to produce a dark brown precipitate;

[0073] (3) Wash the precipitate in step (2) with deionized water and alcohol, centrifuge and freeze-dry to obtain spherical nanoparticles, that is, metal-catechin composite nanomaterials.

[0074] The metal-catechin composite nanomaterial prepared above was applied to inhibit the M1 polarization of THP-1 cells, and the steps were as follows:

[0075] (1) Weigh 50 mg of metal-catechin composite nanomaterials, disperse them in 10 mL of phosphate buffered saline (PBS) after sterilization to obtain metal-catechin composite nanomaterial dispersi...

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Abstract

The invention relates to a metal-catechin composite nano-material as well as a preparation method and application thereof. The metal-catechin composite nano-material is spherical nano-particles formed by coordination binding of metal ions and catechin, and the diameter of the metal-catechin composite nano-material is 5-500 nm. According to the principle of metal-organic coordination, a metal salt solution and a catechin solution are mixed and stirred at 20-80 DEG C to prepare the metal-catechin composite nano-material. The stability of the prepared metal-catechin composite nano-material is remarkably improved, meanwhile, the metal-catechin composite nano-material can be easily endocytosed by cells with uptake capacity such as stem cells, immune cells and tumor cells, catechin molecules can be efficiently released, the metal-catechin composite nano-material participates in the intracellular process, and the metal-catechin composite nano-material further has good degradability. Meanwhile, the nano-material has high bone formation promoting and fat formation inhibiting efficiency, can be used for bone defect repair based on bone tissue engineering of stem cells, can also be used as an anti-fat-forming agent to inhibit inflammatory response of macrophages, can be used for anti-inflammatory treatment of inflammatory diseases, and can also be used for bone tissue repair assisted by the macrophages.

Description

technical field [0001] The invention relates to a metal-catechin composite nano material and its preparation method and application, belonging to the technical field of biomaterials. Background technique [0002] Tea polyphenols are the first botanical drug approved by the US Food and Drug Administration (FDA). They are safe in application and have excellent biocompatibility and degradability. Catechin is a kind of green tea polyphenols, mainly containing four components: epigallocatechin (EGC), epicatechin (EC), epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). Catechin can promote osteogenic differentiation of mesenchymal stem cells, inhibit adipogenesis, and exhibit anti-obesity effects in animals and humans; it also has various physiological functions such as anti-oxidation, anti-inflammation, inhibition of bacteria and viruses, and anti-tumor , has broad biomedical application prospects. In addition, catechins are low cost, biodegradable, and have good b...

Claims

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Application Information

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IPC IPC(8): C07D311/62A61K31/555A61P19/00A61P29/00C12N5/0775C12Q1/02
CPCC07D311/62A61K31/555A61P19/00A61P29/00C12N5/0667G01N33/6863C12N2500/30
Inventor 王书华孔颖刘锋桑元华李刚薛皓刘宏
Owner SHANDONG UNIV
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