Process for extracting effective components from culture medium after ganoderma lucidum picking
A technology of active ingredients and culture medium, which is applied in the field of extracting active ingredients of Ganoderma lucidum, can solve the problems of effective ingredients of Ganoderma lucidum not being effectively extracted, wasting, and discarding.
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Embodiment 1
[0037] This embodiment provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum, comprising the following steps:
[0038] (1) Select the culture medium without bacteria contamination after picking Ganoderma lucidum, pour water on the surface, and place it under sterile dark conditions at 25°C for 2 days, collect the colonies on the surface of the medium, and culture them repeatedly for 3 times, then combine Bacterial colonies on the surface of the culture medium to obtain ganoderma lucidum colonies;
[0039] (2) Washing the culture medium obtained in step (1) to obtain a washing liquid, standing the washing liquid for 1 hour, removing the sediment at the bottom, and obtaining a suspension containing Ganoderma lucidum mycelium;
[0040] (3) Separating and purifying the mycelia of Ganoderma lucidum in the suspension obtained in step (2) to obtain purified mycelia of Ganoderma lucidum;
[0041] (4) Combine Ganoderma lucidum col...
Embodiment 2
[0043] On the basis of Example 1, this example also provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum. The washing treatment method of the medium in step (2) is as follows:
[0044] S1. Rinse the medium obtained in step (1) with water twice, spread it out in an open container, and keep the thickness of the medium in the container at 1 cm;
[0045]S2. Add fine sand to the culture medium in the container, after stirring evenly, spray castor oil in the culture medium with a nozzle, then stir evenly, then transfer the material into the elution column, and use 8 times the amount of 60% ethanol solution Elution was performed twice, and the eluents were combined;
[0046] S3. Distill the eluate obtained in step S2 to remove ethanol under reduced pressure, then place it in a separation funnel, and collect the lower layer liquid to obtain the washing liquid.
[0047] The mass ratio of the culture medium in the container to fin...
Embodiment 3
[0051] On the basis of Example 1, this example also provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum, and the separation and purification treatment method of Ganoderma lucidum hyphae in step (3) is as follows:
[0052] A. Add 1 / 3 mass of Ganoderma lucidum colonies to the suspension obtained in step (2), then mix evenly, and disperse in a 400r / min disperser for 20 minutes to obtain a seed suspension;
[0053] B, gelatin is mixed with 6 times of water to make a gel, then the gel is passed through a granulator to make 20 mesh gel granules, and then the surface of the gel granules is covered with gauze to obtain a fixed carrier;
[0054] C. Inoculate the seed suspension into the liquid medium according to the inoculation amount of 10%, then add the fixed carrier, and then place it on a shaker at 24°C and 180r / min for culture until the ganoderma lucidum mycelium aggregates on the fixed carrier and grows to form a stable My...
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Abstract
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