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Process for extracting effective components from culture medium after ganoderma lucidum picking

A technology of active ingredients and culture medium, which is applied in the field of extracting active ingredients of Ganoderma lucidum, can solve the problems of effective ingredients of Ganoderma lucidum not being effectively extracted, wasting, and discarding.

Inactive Publication Date: 2021-07-06
JIANGXI XIANKELAI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And because the fruiting body of Ganoderma lucidum is derived from the growth and development of Ganoderma lucidum hyphae, there are often Ganoderma lucidum mycelia left on the culture medium after Ganoderma lucidum is cultivated, but at present, the culture medium after picking Ganoderma lucidum is mostly discarded and not used. The active ingredients of Ganoderma lucidum have not been effectively extracted, resulting in waste
However, although some manufacturers process and extract Ganoderma lucidum together with the culture medium when picking Ganoderma lucidum, impurities in the culture medium are introduced into the Ganoderma lucidum extract, especially the polysaccharides in the raw material of the culture medium will be used as Ganoderma lucidum polysaccharides, resulting in effective Ganoderma lucidum. The purity of the ingredients is not enough, which affects the efficacy of the medicine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum, comprising the following steps:

[0038] (1) Select the culture medium without bacteria contamination after picking Ganoderma lucidum, pour water on the surface, and place it under sterile dark conditions at 25°C for 2 days, collect the colonies on the surface of the medium, and culture them repeatedly for 3 times, then combine Bacterial colonies on the surface of the culture medium to obtain ganoderma lucidum colonies;

[0039] (2) Washing the culture medium obtained in step (1) to obtain a washing liquid, standing the washing liquid for 1 hour, removing the sediment at the bottom, and obtaining a suspension containing Ganoderma lucidum mycelium;

[0040] (3) Separating and purifying the mycelia of Ganoderma lucidum in the suspension obtained in step (2) to obtain purified mycelia of Ganoderma lucidum;

[0041] (4) Combine Ganoderma lucidum col...

Embodiment 2

[0043] On the basis of Example 1, this example also provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum. The washing treatment method of the medium in step (2) is as follows:

[0044] S1. Rinse the medium obtained in step (1) with water twice, spread it out in an open container, and keep the thickness of the medium in the container at 1 cm;

[0045]S2. Add fine sand to the culture medium in the container, after stirring evenly, spray castor oil in the culture medium with a nozzle, then stir evenly, then transfer the material into the elution column, and use 8 times the amount of 60% ethanol solution Elution was performed twice, and the eluents were combined;

[0046] S3. Distill the eluate obtained in step S2 to remove ethanol under reduced pressure, then place it in a separation funnel, and collect the lower layer liquid to obtain the washing liquid.

[0047] The mass ratio of the culture medium in the container to fin...

Embodiment 3

[0051] On the basis of Example 1, this example also provides a process for extracting active ingredients from the culture medium after picking Ganoderma lucidum, and the separation and purification treatment method of Ganoderma lucidum hyphae in step (3) is as follows:

[0052] A. Add 1 / 3 mass of Ganoderma lucidum colonies to the suspension obtained in step (2), then mix evenly, and disperse in a 400r / min disperser for 20 minutes to obtain a seed suspension;

[0053] B, gelatin is mixed with 6 times of water to make a gel, then the gel is passed through a granulator to make 20 mesh gel granules, and then the surface of the gel granules is covered with gauze to obtain a fixed carrier;

[0054] C. Inoculate the seed suspension into the liquid medium according to the inoculation amount of 10%, then add the fixed carrier, and then place it on a shaker at 24°C and 180r / min for culture until the ganoderma lucidum mycelium aggregates on the fixed carrier and grows to form a stable My...

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Abstract

The invention discloses a process for extracting effective components from a culture medium after ganoderma lucidum picking, which comprises the following steps: (1) selecting the culture medium without infectious microbe pollution after ganoderma lucidum picking, spraying water on the surface, culturing for 2-4 days, and collecting bacterial colonies on the surface; (2) carrying out washing treatment on the obtained culture medium, and standing the washing solution to remove the bottom precipitate, so as to obtain a suspension containing ganoderma lucidum hyphae; (3) separating and purifying the ganoderma lucidum hyphae in the suspension to obtain purified ganoderma lucidum hyphae; and (4) combining the bacterial colonies and the ganoderma lucidum hyphae, then performing water extraction for 3-5 times, combining extracting solutions, performing standing to remove precipitate at the bottom layer, and taking supernate, thereby the extracting solution of the effective components in the culture medium after the ganoderma lucidum pickling is obtained. According to the method, the ganoderma lucidum hyphae in the culture medium after the ganoderma lucidum is picked are firstly collected and then are separated and purified, finally, the effective components are extracted, the process is simple, the design is reasonable, the extracted effective components are all from the ganoderma lucidum, and impurity components in the culture medium are not introduced.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of ganoderma, and more specifically relates to a process for extracting active ingredients from culture medium after picking ganoderma. Background technique [0002] Ganoderma lucidum, umbrella-shaped in shape, with a kidney-shaped, semicircular or nearly round cap, is the fruiting body of the polyporaceae fungus Ganoderma lucidum, and is one of the famous edible and medicinal fungi in my country. Modern medicine has proved that Ganoderma lucidum contains a variety of physiological regulatory factors that have obvious effects on the human body, and the most important active ingredients are Ganoderma lucidum polysaccharide and Ganoderma acid. Ganoderma lucidum polysaccharides mainly refer to monosaccharide polymers with physiological activity. Ganoderma lucidum polysaccharides have the functions of anti-tumor, improving the immune activity of the body, scavenging free radicals...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/074C12N1/14C12R1/645
CPCA61K36/074C12N1/14A61K2236/331
Inventor 潘新华潘峰郭培花
Owner JIANGXI XIANKELAI BIOLOGICAL TECH CO LTD