Stable double-reagent blood ammonia determination kit
A blood ammonia determination, dual-reagent technology, applied in the preparation of test samples, measurement of color/spectral properties, analysis by chemical reaction of materials, etc., can solve the positive error of the measured value, blank AMM interference, difficult Eliminate problems such as
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Embodiment 1
[0125] Embodiment 1: Preparation of blood ammonia determination kit
[0126] R1 is composed as follows:
[0127]
[0128]
[0129] Preparation of reagent R1: Add the whole batch of purified water into the container, add the whole batch of tris, α-KG, disodium glutarate, ATP, TritonX-100, MIT in sequence, and stir until completely dissolved Adjust the pH to 8.5±0.05 (25±0.5°C), then add the whole batch of L-GS, NADH, and LDH in sequence, stir until completely dissolved, and then filter with a 0.22 μM filter membrane.
[0130] R2 is composed as follows:
[0131] name concentration bis-tris, pH6.0 20mmol / L Glutaric acid disodium salt 5mmol / L ATP 2mmol / L L-GS 5U / L GLDH 20KU / L Mannitol 1％ brij-35 0.1％ NaN 3
[0132] Preparation of reagent R2: Add the whole batch of purified water into the container, and then add the whole batch of bis-tris, glutaric acid disodium salt, ATP, mannitol, brij-35, NaN into the cont...
Embodiment 2
[0133] Embodiment 2: Preparation of blood ammonia determination kit
[0134] R1 is composed as follows:
[0135]
[0136]
[0137] Preparation of reagent R1: Add the whole batch of purified water into the container, add the whole batch of glycine / sodium hydroxide, α-KG, disodium glutarate, ATP, Tween-20, CAA in sequence, and then Stir until completely dissolved, then adjust the pH to 8.5±0.05 (25±0.5°C), then add the whole batch of L-GS, NADH, and LDH in sequence, stir until completely dissolved, and filter with a 0.22μM filter.
[0138] R2 is composed as follows:
[0139] name concentration PIPES, pH6.5 50mmol / L Glutaric acid disodium salt 10mmol / L ATP 1mmol / L L-GS 3U / L GLDH 60KU / L BSA 2％ OP-10 0.05％ CAA 0.1％
[0140] Preparation of reagent R2: Add the whole batch of purified water into the container, add the whole batch of PIPES, glutaric acid disodium salt, ATP, BSA, OP-10, CAA in sequence, and sti...
Embodiment 3
[0141] Embodiment 3: Preparation of blood ammonia determination kit
[0142] R1 is composed as follows:
[0143] name concentration AMPSO, pH8.6 100mmol / L alpha-ketoglutarate 5mmol / L Glutaric acid disodium salt 8mmol / L ATP 5mmol / L L-GS 6U / L NADH 0.45mmol / L LDH 1KU / L Tween 80 0.02％ IZU 0.2％
[0144]Preparation of reagent R1: Add the whole batch of purified water into the container, add the whole batch of AMPSO, α-KG, disodium glutarate, ATP, Tween-80, IZU in sequence, and stir until completely dissolved Adjust the pH to 8.6±0.05 (25±0.5°C), then add the whole batch of L-GS, NADH, and LDH in sequence, stir until completely dissolved, and then filter with a 0.22 μM filter membrane.
[0145] R2 is composed as follows:
[0146] name concentration MES, pH6.0 100mmol / L Glutaric acid disodium salt 8mmol / L ATP 5mmol / L L-GS 6U / L GLDH 30KU / L BSA 2％ brij...
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