Chip for simply, conveniently and rapidly discretizing liquid sample and use method of chip
A discretization and chip technology, applied in chemical instruments and methods, sampling, laboratory containers, etc., can solve the problems of high cost, poor reliability, and complicated operation, and achieve low loss rate and low-cost discretization Effect of treatment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] A simple and fast discrete chip for liquid samples is applied to digital PCR analysis. Taking the detection of EGFR L858R mutation in peripheral blood of lung cancer patients as an example, the specific steps are as follows:
[0030] (1) Chip preparation: firstly, the chip mold is made by photolithography process. The specific process is: spin-coat SU8-3010 negative photoresist (500rpm, 12s; 1000rpm, 40s) on a clean 4-inch silicon wafer, and let it stand for 1 -2h, after pre-baking (95°C, 15min), exposure (3min), post-baking in the dark (93.5°C, 3min), natural cooling and development (2min), hard baking (170°C, 30min), the chip mold was made The graphic structure of the first layer (corresponding to the chip pipeline layer); after the silicon chip is cooled, spin-coat SU8-3050 negative photoresist on the graphic structure (500rpm, 12s; 1000rpm, 40s), and let it stand for 2-3h. After pre-baking (95°C, 30min), exposure (3min 40s), post-baking in the dark (93.5°C, 5min), n...
Embodiment 2
[0039] A simple and fast discrete chip for liquid samples, which is applied to the rapid counting of microbial colonies, taking the detection of Escherichia coli as an example, the specific steps are as follows:
[0040] (1) Chip preparation: The hydrophobic microstructure layer of the chip is fabricated by photolithography molding process combined with PDMS pouring mold and oxygen plasma surface treatment bonding process. The hydrophobic microstructure layer contains about 40,000 microcavities and about 121,000 connecting phases Micro-channels adjacent to micro-cavities, wherein each micro-cavity is cylindrical in shape, 100 microns in diameter, 100 microns in depth, and 20 microns in width and height. A 2mm-thick chip PDMS cover sheet layer for the sample port and an oil storage chamber; then, align and bond the cover sheet layer and the hydrophobic microstructure layer, and assemble to obtain a liquid phase rapid discretization chip; the specific process of chip preparation ...
Embodiment 3
[0048] A simple and fast discrete chip for liquid samples is applied to the synthesis of micro-nano particles. Taking the synthesis of polyethylene glycol diacrylate (PEGDA) gel microspheres as an example, the specific steps are as follows:
[0049] (1) Chip preparation: A PDMS hydrophobic microstructure layer containing 20,000 microcavities and a connected micropipe network was fabricated by photolithography molding process combined with PDMS pouring mold, wherein each microcavity was cylindrical in shape with a diameter of 100 Micron, the depth is 100 microns; at the same time, a 2 mm thick chip PDMS cover sheet layer containing a sample inlet and an oil storage cavity is made by pouring and drilling through PDMS; then, align the cover sheet layer with the hydrophobic microstructure layer, Bonding and assembling to obtain a liquid-phase rapid discrete chip; the specific process of chip preparation refers to Example 1;
[0050] (2) Chip degassing: place the chip obtained in s...
PUM
| Property | Measurement | Unit |
|---|---|---|
| thickness | aaaaa | aaaaa |
| thickness | aaaaa | aaaaa |
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More