Method for producing bacterial cellulose through coconut milk pre-fermentation

A technology of bacterial cellulose and pre-fermentation, applied in the directions of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of slow growth and reproduction of Acetobacter xylinum, high transportation cost of coconut water, hard coconut tissue structure, etc. problems, to achieve the effect of omitting the embrittlement step, solving the shortage of raw material resources and making the taste crisp and tender

Inactive Publication Date: 2021-07-09
湖北香园食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. Coconut water resources in China are not enough for fresh food supply, and almost no surplus is used to make coconuts;
[0004] 2. The transportation cost of coconut water is high;
[0005] 3. The tissue structure of the coconut fruit fermented with coconut water is relatively hard, and must be embrittled after post-processing;
[0006] 4. Due to the high fat content of fresh coconut milk, if it is directly used as a fermentation medium, the oil will float on the surface of the medium to isolate oxygen, making the medium oxygen insufficient, and the Acetobac

Method used

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  • Method for producing bacterial cellulose through coconut milk pre-fermentation

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Comparison scheme
Effect test

Embodiment 1

[0030] S1, first fresh coconut meat is defibrinated to obtain fresh coconut milk, and fresh coconut meat is to obtain fresh coconut meat by peeling and scraping the coconut fruit picked for 2 days;

[0031] S2. Pour the fresh coconut milk obtained in step S1 into cooking equipment, boil and sterilize the coconut milk, then remove it and cool it to room temperature, then transfer it to a fermenter and insert yeast for fermentation. After 24 hours of fermentation, the coconut milk oil will float. Stratification occurs, remove the floating oil with a scraper tool, and collect the supernatant;

[0032] S3. Transfer the supernatant liquid obtained in step S2 to the batching equipment, and then respectively prepare white granulated sugar, small ingredients and water in corresponding weight ratios through the batching equipment, and mix the supernatant liquid, white granulated sugar, small ingredients and water of the batching Pour water into the mixing equipment one by one, start th...

Embodiment 2

[0036] S1, first fresh coconut meat is defibrinated to obtain fresh coconut milk, and fresh coconut meat is to obtain fresh coconut meat by peeling and scraping the coconut fruit picked for 1 day;

[0037] S2. Pour the fresh coconut milk obtained in step S1 into cooking equipment, boil and sterilize the coconut milk, then remove and air-cool to room temperature, then transfer to a fermenter and insert yeast for fermentation. After 23 hours of fermentation, the coconut milk oil floats, Stratification occurs, remove the floating oil with a scraper tool, and collect the supernatant;

[0038] S3. Transfer the supernatant liquid obtained in step S2 to the batching equipment, and then respectively prepare white granulated sugar, small ingredients and water in corresponding weight ratios through the batching equipment, and mix the supernatant liquid, white granulated sugar, small ingredients and water of the batching Pour water into the mixing equipment one by one, start the mixing e...

Embodiment 3

[0042] S1, at first the fresh coconut meat is refined to obtain fresh coconut milk, and the fresh coconut meat is obtained by peeling and scraping the coconut fruit picked for 3 days;

[0043] S2, pour the fresh coconut milk obtained in step S1 into the cooking equipment, boil the coconut milk to sterilize, then remove and air-cool to room temperature, then transfer to the fermenter and insert yeast to ferment, after 25 hours of fermentation, the coconut milk grease floats, Stratification occurs, remove the floating oil with a scraper tool, and collect the supernatant;

[0044]S3. Transfer the supernatant liquid obtained in step S2 to the batching equipment, and then respectively prepare white granulated sugar, small ingredients and water in corresponding weight ratios through the batching equipment, and mix the supernatant liquid, white granulated sugar, small ingredients and water of the batching Pour water into the mixing equipment one by one, start the mixing equipment and...

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Abstract

The invention discloses a method for producing bacterial cellulose by pre-fermentation of coconut milk, which specifically comprises the following steps: S1, firstly grinding fresh coconut meat into thick liquid to obtain fresh coconut milk, S2, preparing a lower clear liquid, S3, blending a culture medium, S4, sterilizing the culture medium blended in the step S3 for 10-15 minutes by high-pressure steam sterilization equipment, then taking out and loading into a tray, and cooling to room temperature; and S5, inoculating a production strain into the culture medium cooled in the step S4, fermenting for 2-3 days, and then extracting and harvesting the bacterial cellulose. The method relates to the technical field of bacterial cellulose fermentation. The method for producing bacterial cellulose through pre-fermentation of coconut milk solves the problem of insufficient coconut water raw material resources for coconut fermentation, coconut pulp is more convenient to transport and lower in transportation cost than coconut water, and compared with the prior art, the method omits the embrittlement step, simplifies the process, saves the processing cost and improves the processing efficiency; and the economic value of the coconut meat can be maximized.

Description

technical field [0001] The invention relates to the technical field of bacterial cellulose fermentation, in particular to a method for producing bacterial cellulose by pre-fermentation of coconut milk. Background technique [0002] Bacterial cellulose refers to the general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. There are many ways to cultivate bacterial cellulose. Such as static culture and dynamic culture, cellulose with different advanced structures can be obtained by using acetic acid bacteria, and bacterial cellulose with different chemical properties can also be obtained by adjusting the culture conditions, for example, adding water-soluble polymers such as carboxymethyl to the culture solution Fibers with different microstructures and aggregation behaviors can be obtained by using cellulose-based cellulose, hemicellulose, chitosan, fluorescent dyes, and endogluc...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 余敏华
Owner 湖北香园食品有限公司
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