Method for producing bacterial cellulose through coconut milk pre-fermentation

Abstract

The invention discloses a method for producing bacterial cellulose by pre-fermentation of coconut milk, which specifically comprises the following steps: S1, firstly grinding fresh coconut meat into thick liquid to obtain fresh coconut milk, S2, preparing a lower clear liquid, S3, blending a culture medium, S4, sterilizing the culture medium blended in the step S3 for 10-15 minutes by high-pressure steam sterilization equipment, then taking out and loading into a tray, and cooling to room temperature; and S5, inoculating a production strain into the culture medium cooled in the step S4, fermenting for 2-3 days, and then extracting and harvesting the bacterial cellulose. The method relates to the technical field of bacterial cellulose fermentation. The method for producing bacterial cellulose through pre-fermentation of coconut milk solves the problem of insufficient coconut water raw material resources for coconut fermentation, coconut pulp is more convenient to transport and lower in transportation cost than coconut water, and compared with the prior art, the method omits the embrittlement step, simplifies the process, saves the processing cost and improves the processing efficiency; and the economic value of the coconut meat can be maximized.

Description

technical field [0001] The invention relates to the technical field of bacterial cellulose fermentation, in particular to a method for producing bacterial cellulose by pre-fermentation of coconut milk. Background technique [0002] Bacterial cellulose refers to the general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. There are many ways to cultivate bacterial cellulose. Such as static culture and dynamic culture, cellulose with different advanced structures can be obtained by using acetic acid bacteria, and bacterial cellulose with different chemical properties can also be obtained by adjusting the culture conditions, for example, adding water-soluble polymers such as carboxymethyl to the culture solution Fibers with different microstructures and aggregation behaviors can be obtained by using cellulose-based cellulose, hemicellulose, chitosan, fluorescent dyes, and endogluc...

AI Technical Summary

Problems solved by technology

[0003] 1. Coconut water resources in China are not enough for fresh food supply, and almost no surplus is used to make coconuts;

[0004] 2. The transportation cost of coconut water is high;

[0005] 3. The tissue structure of the coconut fruit fermented with coconut water is relatively hard, and must be embrittled after post-processing;

[0006] 4. Due to the high fat content of fresh coconut milk, if it is directly used as a fermentation medium, the oil will float on the surface of the medium to isolate oxygen, making the medium oxygen insufficient, and the Acetobacter xylinum used for fermentation is an aerobic bacterium, which will It directly causes the growth and reproduction of Acetobacter xylinum to slow down. Even if the oil is removed, part of the nutrients in the coconut milk will be removed along with the fat, resulting in insufficient nutrition in the coconut milk. Sufficient nutrition cannot be obtained in the culture medium made of pulp, the growth metabolism is slow, and the cellulose produced is less. When the fermentation is finished, the residual medium in the fermentation fruit plate is more than 50%.

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