Composite microbial preparation, preparation method and application

A technology of compound microorganisms and compound preparations, applied in the field of microorganisms, can solve the problems of disease prevention and growth promotion, limited growth promotion effect, environmental pollution, pesticide residues and pathogenic bacteria resistance, etc., so as to promote the healthy growth of plants and prevent and control the occurrence of anthracnose. , the effect of increasing the income of fruit farmers

Active Publication Date: 2021-07-16
金禾佳农(北京)生物技术有限公司
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  • Application Information

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Problems solved by technology

The prevention and control of citrus diseases in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as environmental pollution, pesticide residues, and resistance of pathogenic bacteria, which are difficult to solve.
A

Method used

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  • Composite microbial preparation, preparation method and application
  • Composite microbial preparation, preparation method and application
  • Composite microbial preparation, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0027] Example 1 Screening and culture of each strain of the hyperiothermal

[0028] (1) Screening of Bacillus Bacillus Bacillus JNKC001

[0029] Take fresh infected anthrax 10g to ground into a paste, adding a sterilized tapered bottle with 90 ml of sterile water, 180R / min, thoroughly shaking 30 min, and taking the supernatant. Mix the strains of each strain screened from the soil for 15 min, and the anthrax juice is a positive control. The local sphere is vaccinated, and the long-term-consistent central blade is selected. The routine friction inoculation method is used, and the left is treated. On the right, the control, each process 5 leaf, repeated 3 times, tagged, observe the incidence of the recording leaf. The strains of the least incidence were selected from the bacillus bracicus JNKC001.

[0030] (2) Cultivation of Bacillus JNKC001 of Bacillus Subtilis

[0031] 1) Take the activated Bacillus JNKC001, inoculated in seed medium, under conditions of 28 to 33 ° C, the rotat...

Example Embodiment

[0050] Example 2 Preparation of Composite Microbial Formulations

[0051] 1) The concentration is 3 * 10 9 CFU / ml of Bacillus JNKC001 and 1.5 * 10 8 CFU / ml Harzianum JNHC is mixed according to a 10: 2 ratio (volume ratio), and the stock solution of the composite preparation is prepared according to the 10: 2 ratio (volume ratio);

[0052] 2) Add 6-10% of humic acid, 70-80%, wetting agent from 10 to 8%, stabilizer from 1 to 2%, to obtain a composite microorganism formulation.

[0053] The possible composition of the complex formulation can also be represented in the form of a table (each 100 g of composite formulations), as in Table 1:

[0054]

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Abstract

The invention discloses a composite microbial preparation, which comprises a bacillus subtilis JNKC001 bacterial liquid, a trichoderma harzianum JNHC bacterial liquid and an auxiliary agent, and the ratio of the total bacterial count of the trichoderma harzianum to the total bacterial count of the bacillus subtilis in the prepared composite microbial preparation is (1-3):(100-300). The compound microbial preparation can effectively improve the content of chlorophyll in citrus leaves; the citrus anthracnose is effectively prevented and controlled; the content of soluble solids in the citrus is increased; the Vc content of the citrus is increased; and the composite microbial preparation has good growth promoting and yield promoting effects on the citrus.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a composite microbial preparation, preparation method and application. Background technique [0002] Citrus is the world's largest fruit and the third largest agricultural product in the world. In recent years, the market demand for citrus in various regions has increased year by year, and the corresponding planting area has also increased year by year. However, due to the increase in planting area, citrus leaf yellowing and diseases such as anthracnose are becoming more and more prominent. As an excellent variety of citrus, Shatangju has a relatively large planting area, and the problem that restricts the development of the citrus industry is more obvious in Shatangju. The prevention and control of citrus diseases in China is mainly based on chemical control. As we all know, the long-term and large-scale use of chemical pesticides often leads to many problems such as env...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/22A01N63/38A01P3/00A01P21/00C12R1/125C12R1/885
CPCC12N1/20C12N1/14A01N63/22A01N63/38
Inventor 张丽霞
Owner 金禾佳农(北京)生物技术有限公司
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