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A method of propagating Paeoniae officinalis by tissue culture technique

A tissue culture and peony technology is applied in the field of propagating peony by inducing tissue culture seedlings to produce a bud tray structure by using tissue culture technology, and can solve the problems of affecting the transplanting of peony in vitro seedlings, restricting the development process of peony, weak growth potential of tissue culture seedlings, and the like, Achieve the effect of being conducive to large-scale production, less vitrification, and greater root vigor

Active Publication Date: 2022-04-22
河南省农业科学院园艺研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, peonies are mainly propagated by ramets, but only in autumn, which is limited by time and the reproduction efficiency is low.
There have been literatures on tissue culture techniques of different varieties of peony, but most of the studies focus on the selection of explants and the formulation of the medium, and all of them proliferate by inducing the formation of adventitious buds, and there are phenomena such as browning and vitrification. The vitrification rate is as high as 40.91% (Zheng Liwen et al., Research on in vitro bud culture and vitrified seedling rejuvenation of peony 'seed powder', Forestry Science Research, 2011, 24 (3): 379-384); tissue culture seedlings have weak growth potential, The highest rooting rate is 28.15%, and a large number of callus tissues are formed at the base of the seedlings, and the callus rate is as high as 44.4% (Wu Hongjuan et al., Induction and rooting technology of ornamental peony 'Da Fugui' cluster buds, Journal of Northeast Forestry University, 2011 (9): 20-22), seriously affected the transplanting of test-tube seedlings of peony, became the technical bottleneck of tissue culture and rapid propagation of peony, and restricted the development of the industrialization of peony

Method used

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  • A method of propagating Paeoniae officinalis by tissue culture technique
  • A method of propagating Paeoniae officinalis by tissue culture technique
  • A method of propagating Paeoniae officinalis by tissue culture technique

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A method of utilizing tissue culture technology to propagate Radix Paeoniae Alba, comprising the steps of:

[0048] 1) Sterilization of peony explants: From January to February, collect the underground scale buds of peony as explants (see figure 1 ), cleaning, and sterilization (specifically: first rinse with tap water, then scrub the scales with detergent, and finally rinse with running water for 30 minutes; take it to the ultra-clean workbench for sterilization, with a volume concentration of 75% alcohol Sterilize for 30 seconds with 0.1% HgCl 2 Sterilize for 10 minutes, rinse with sterile water once, change to a sterile bottle, and then use HgCl with a mass concentration of 0.1% 2 Sterilize for 8 minutes, rinse with sterile water 3 to 4 times, and finally rinse with 250 mg / L cephalosporin sterile water once);

[0049] 2) Germination of peony explants: place the sterilized scale buds on sterile filter paper to absorb the water, peel off the peripheral scales with st...

Embodiment 2

[0058] The difference test of the culture effect brought by different medium composition is given below.

[0059] 1. Screening of explant germination medium

[0060] Insert the sterilized peonies stem tips into the explant germination medium, place them in the culture room for cultivation, the cultivation temperature is 23±2°C, the light intensity is 2000-3000Lx, and the light time is 12 hours / day. The explant germination medium uses MS medium as the basic medium, in which the macroelements are halved, that is, 1 / 2 MS medium, 30 g / L of sucrose and 6 g / L of agar are added, and different types and concentrations of plant growth are added at the same time. Regulate substances (see Table 1 for details), observe their effects on the germination of peony explants, count the germination rate 40 days after grafting and transfer, enter the latent bud induction link, germination rate = number of plants with germinated leaves / no pollution in this treatment The total number of plants * 1...

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Abstract

The invention relates to a method for propagating peony by using tissue culture technology, which uses the subterranean scale buds of peony as the source of explants, cleans and sterilizes them as explants for tissue culture; and then cultures 20- For 40 days, cultivate shoot tips to germinate into seedlings; transfer the tissue cultured seedlings formed by shoot tip germination to the latent bud formation medium and cultivate them for 50‑100 d, so that the latent buds at the petiole base and stem base are formed and grow up; The latent buds were transferred to the bud plate induction medium and cultured for 30-60 days to induce the formation of bud plates. The method is designed for the growth and reproduction characteristics of Paeoniae Alba. It is simple and easy to implement, and can obtain a large number of bud plates. The buds on the upper end of the bud plate can germinate normally, and the lower end of the bud plate can form adventitious roots. Rooted seedlings.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and rapid propagation methods for plants, and in particular relates to a method for inducing tissue culture seedlings to produce a bud plate structure for propagating peonies by using tissue culture technology. Background technique [0002] Peony is a perennial herb of Paeoniaceae Paeoniaceae. It is a traditional famous flower in my country. It has a cultivation history of more than 3,000 years. Peony flowers are large and colorful, and the flower types are changeable. They can not only be cultivated in gardens and gardens, but also high-grade cut flowers. The roots of peony are medicinal, the flowers are edible, and the seeds have a high oil content, rich in linolenic acid, linoleic acid and oleic acid, and can be used as edible oil with broad application prospects. [0003] At present, peonies are mainly propagated by ramets, but only in autumn, which is limited by time and the propagati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李艳敏张和臣薛娴王慧娟高杰符真珠王利民王二强高凯袁欣蒋卉
Owner 河南省农业科学院园艺研究所