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High-stability magnetic immobilized laccase, preparation method thereof and method for efficiently degrading dye

An immobilized laccase, high stability technology, applied in biochemical equipment and methods, immobilized on or in inorganic carriers, chemical instruments and methods, etc. Improved dispersion, improved metal ion tolerance, and simple preparation methods

Pending Publication Date: 2021-07-23
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various magnetically immobilized enzymes such as Fe 3 o 4 / SBA-15, Fe 3 o 4 @MIL-100(Fe), Fe 3 o 4 @SiO2 (J.Oleo Sci.63, (10) 1027-1034 (2014); Catalysts 2019, 9, 850 (2019); Microporous and Mesoporous Materials 197 (2014) 1-7), but the laccase in the literature is currently reported in the immobilization process A large amount of enzyme activity is lost in the process, and it cannot be widely promoted in industrial production

Method used

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  • High-stability magnetic immobilized laccase, preparation method thereof and method for efficiently degrading dye
  • High-stability magnetic immobilized laccase, preparation method thereof and method for efficiently degrading dye
  • High-stability magnetic immobilized laccase, preparation method thereof and method for efficiently degrading dye

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A preparation method for highly stable magnetically immobilized laccase, comprising the following steps:

[0080] 1) Preparation of magnetic Fe 3 o 4 The operation process of nanoparticles and amino functionalization is as follows:

[0081] 1-1) Weigh 1.35g FeCl 3 ·6H 2 O, 3.6g NaAc 3H 2O is placed in a polytetrafluoroethylene liner, add 40mL ethylene glycol and 1mL PEG-200, stir evenly to obtain a yellow emulsion, react the reactor at 190°C for 10h, cool to room temperature; wash the product once with ethanol, Wash twice with distilled water and separate to obtain magnetic Fe 3 o 4 Nanoparticles;

[0082] 1-2) Prepare Fe from 1-1) 3 o 4 Disperse the nanoparticles in 60mL of distilled water, add 3mL of 3-aminopropyltriethoxysilane, heat up to 140°C with protective gas argon and reflux for 23h, wash with distilled water once, and separate to obtain aminated magnetic Fe 3 o 4 Nanoparticles.

[0083] 2) Aminated magnetic Fe 3 o 4 The operation process of nano...

Embodiment 2

[0100] A preparation method for highly stable magnetically immobilized laccase, comprising the following steps:

[0101] 1) Preparation of aminated magnetic Fe 3 o 4 The operation process of nanoparticles is as follows:

[0102] 1-1) Weigh 1.35g FeCl 3 ·6H 2 O, 3.6g NaAc 3H 2 O is placed in a polytetrafluoroethylene liner, add 40mL ethylene glycol and 1mL PEG-200, stir evenly to obtain a yellow emulsion, react the reactor at 220°C for 10h, cool to room temperature; wash the product once with ethanol, Wash twice with distilled water and separate to obtain magnetic Fe 3 o 4 Nanoparticles;

[0103] 1-2) Prepare Fe from 1-1) 3 o 4 Disperse the nanoparticles in 60mL of distilled water, add 3mL of 3-aminopropyltriethoxysilane, heat up to 130°C with protective gas argon and reflux for 20h, wash with distilled water once, and separate to obtain aminated magnetic Fe 3 o 4 Nanoparticles.

[0104] 2) The operation process of immobilized laccase is as follows:

[0105] 2-1) t...

Embodiment 3

[0112] A preparation method for highly stable magnetically immobilized laccase, comprising the following steps:

[0113] 1) Preparation of aminated magnetic Fe 3 o 4 The operation process of nanoparticles is as follows:

[0114] 1-1) Weigh 1.35g FeCl 3 ·6H 2 O, 3.6g NaAc 3H 2 O is placed in a polytetrafluoroethylene liner, add 40mL ethylene glycol and 1mL PEG-200, stir evenly to obtain a yellow emulsion, react the reactor at 160°C for 19h, cool to room temperature; wash the product once with ethanol, Wash twice with distilled water and separate to obtain magnetic Fe 3 o 4 Nanoparticles;

[0115] 1-2) Prepare Fe from 1-1) 3 o 4 Disperse the nanoparticles in 60mL of distilled water, add 3mL of 3-aminopropyltriethoxysilane, heat up to 120°C with protective gas argon and reflux for 26h, wash with distilled water once, and separate to obtain aminated magnetic Fe 3 o 4 Nanoparticles.

[0116] 2) The operation process of immobilized laccase is as follows:

[0117] 2-1) t...

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Abstract

The invention provides high-stability magnetic immobilized laccase, a preparation method thereof and a method for efficiently degrading dye. The preparation method comprises the following steps of: firstly, synthesizing magnetic Fe3O4 nanoparticles by adopting a solvothermal method; then functionalizing the magnetic nanoparticles by using amino functional groups; and finally, crosslinking by using a bifunctional reagent glutaraldehyde to prepare the magnetic immobilized laccase with high activity, high stability and high reusability. Compared with the prior art, the magnetic immobilized laccase prepared by the preparation method provided by the invention is simple to operate, economic and environment-friendly, high in atom utilization rate, green and pollution-free; and the magnetic immobilized laccase has continuous and stable catalytic ability, simple and convenient reusability and green sustainability, and the production cost is greatly reduced, so that the magnetic immobilized laccase has a wide application prospect in the fields of catalytic oxidation, water body treatment, environmental remediation and the like.

Description

technical field [0001] The invention belongs to the field of dye degradation, and in particular relates to a highly stable magnetically immobilized laccase, a preparation method thereof, and a method for efficiently degrading dyes. Background technique [0002] Laccase (E.C.1.10.3.2), belongs to multi-copper oxidase. Laccases exhibit low specificity and wide substrate range, such as phenolic compounds, diamines, aromatic amines, N-heterocycles, phenothiazines, thiol groups, etc., can be used as reducing substrates for laccases, Moreover, the only by-product of laccase-catalyzed oxidation is water, which is environmentally friendly, so the research on laccase has aroused great interest of scientists. However, there are still difficulties to be overcome in the large-scale application of laccase. The activity of enzyme protein is greatly affected by the environment, and the change of the external physical and chemical environment greatly changes the activity of laccase, and ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C02F103/30
CPCC12N11/14C12N9/0061C02F3/342C12Y110/03002C02F2103/30
Inventor 陈志明朱庆鹏汪春梅
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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