Acid phosphatase optical fiber biosensor as well as preparation method and application thereof
A technology of acid phosphatase and biosensor, applied in instruments, optics, light guides, etc., can solve problems such as false positives and increased refractive index of the surrounding environment of optical fibers
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[0041] S1. Immerse the micro-nano optical fiber interferometer in the piranha solution for 60 minutes to activate the hydroxyl groups on the surface of the optical fiber. Wherein the preparation method of the piranha solution is as follows: take 7 milliliters of concentrated sulfuric acid in a beaker, slowly drop into 3 milliliters of hydrogen peroxide, and stir evenly.
[0042] S2. Dissolve 10 mg of chitosan in 20 ml of sodium acetate buffer (0.05M, pH 5.0) to prepare a 0.5 mg / ml chitosan solution. Similarly, dissolve 10 mg of sodium tripolyphosphate in 20 ml of sodium acetate buffer (0.05M, pH 5.0) to prepare a 0.5 mg / ml sodium tripolyphosphate solution. 0.5mg / ml chitosan solution and 0.5mg / ml sodium tripolyphosphate solution were mixed at a volume ratio of 1:1 to form a suspension.
[0043]S3. Immerse the micro-nano fiber interferometer with activated hydroxyl groups on the surface into the suspension in step S2 for 30 minutes, and modify the surface of the micro-nano fibe...
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