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Attached bioprosthetic valve and preparation method thereof

A bio-valve, fit-type technology, applied in the field of biomedical engineering, can solve problems such as unfavorable endothelial cell adhesion and proliferation, affecting the application effect of heart valves, etc., to improve biocompatibility, maintain aesthetics, and high mechanical properties. Effect

Active Publication Date: 2021-08-03
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the fresh animal valve used in this patent has not undergone decellularization treatment, the cells existing on the surface will have a rejection reaction with the human body, which is also not conducive to the adhesion and proliferation of endothelial cells, thereby affecting the actual application effect of the heart valve.

Method used

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  • Attached bioprosthetic valve and preparation method thereof
  • Attached bioprosthetic valve and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0034] The invention provides a method for preparing a fitting biological valve, comprising the following steps:

[0035] S1. Cutting the decellularized animal valve to obtain animal valve slices 10;

[0036] S2. The polylactic acid fabric woven from the first polylactic acid yarn is fully soaked in the vascular endothelial growth factor solution, and after freeze-drying, the drug controlled release fabric 20 is obtained;

[0037]S3. Cutting the drug controlled release fabric obtained in step S2 so that its size is consistent with the animal valve slice obtained in step S1; and then attaching the two animal valve slices to the drug controlled release fabric respectively The two sides of the valve are bonded with medical bio-glue to obtain a bio-valve stent;

[0038] S4. Acupuncture the biological valve support obtained in step S3 to form a number of holes on the surface of the biological valve support;

[0039] S5. Thread the second polylactic acid yarn 30 sequentially into ...

Embodiment 1

[0049] This embodiment provides a method for preparing a conformable biological valve 100, which includes the following steps:

[0050] S1. Segmentation of animal valves

[0051] Turn on the Leica CM3050S cryostat, adjust the temperature of the sample stage to -40°C, take a fresh decellularized porcine aortic valve as the sample to be cut, blot the surface moisture with filter paper, and measure its thickness with a spiral micrometer; Place the sample to be cut on the sample stage of the cryostat, fix it with embedding agent around the sample to be cut, freeze for 10 minutes, raise the temperature to -15°C, start slicing after 5 minutes, remove the surface embedding agent, and Half of the thickness of the sample to be cut is used as the slice thickness to obtain an animal valve slice 10 with a thickness half of the original thickness of the valve, and save it for future use.

[0052] S2, preparation of drug controlled release fabric

[0053] Soak the polylactic acid fabric i...

Embodiment 2～5

[0063] Embodiments 2 to 5 respectively provide a method for preparing a conformable biological valve. Compared with Embodiment 1, the difference is that the relevant process parameters in steps S2, S4 and S5 are changed, and the remaining steps are the same as those in Embodiment 1. 1 are consistent and will not be repeated here. The parameter values ​​corresponding to each embodiment are shown in Table 1.

[0064] The technological parameter value of table 1 embodiment 2~5

[0065]

[0066] There are certain differences between the mechanical properties and the speed of promoting cell growth of the conforming biological valves of Examples 2-5 prepared according to the above process parameters, indicating that the concentration of growth factors, the warp and weft density of the drug-controlled release fabric and the stitches can be adjusted. The needle spacing regulates the growth rate of cells, and controls the reinforcement effect of the valve by adjusting the sewing ne...

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Abstract

The invention provides an attached bioprosthetic valve and a preparation method thereof. The animal valve subjected to decellularization treatment is cut, the two animal valve slices are attached to the two sides of the drug controlled release fabric adsorbed with the vascular endothelial growth factors respectively, the drug controlled release fabric is clamped between the two animal valve slices, and the biological valve stent is formed under the bonding effect of the medical biological glue, and sewing is performed by using polylactic acid yarns to prepare the attached bioprosthetic valve. By means of the mode, the synergistic effect between a traditional suture technology and a cell growth induction technology can be fully exerted, the mechanical property of the heart valve is enhanced through yarn suture, meanwhile, cells are induced by growth factors to grow towards the interior of the valve along the yarn, endothelialization of the biological valve is promoted, therefore, the attaching type bioprosthetic valve with high mechanical property and high biocompatibility is obtained at the same time. The preparation method provided by the invention is simple in process and can meet the requirements of industrial large-scale production.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a fitting biological valve and a preparation method thereof. Background technique [0002] Heart valve refers to the valve between the atrium and the ventricle or between the ventricle and the artery, which plays an irreplaceable role in the blood circulation of the heart. In each blood circulation process, the heart valve allows blood to pass through the valve opening through its own opening and closing, and prevents the blood from flowing back to the heart, thereby ensuring the normal operation of the physiological system of the human body. However, when the heart valve is damaged or pathological, the structure of the heart valve will be damaged, resulting in a series of symptoms such as fibrosis and adhesion, which in turn will affect the normal blood circulation and physiological functions. At present, heart valve-related diseases are still serious heart disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/54A61L27/56A61L27/50A61F2/24
CPCA61L27/3625A61L27/3683A61L27/18A61L27/54A61L27/56A61L27/50A61F2/24A61L2430/20A61L2300/414A61L2300/602A61F2240/002C08L67/04
Inventor 杨红军王朝荣许愿庄燕徐卫林
Owner WUHAN TEXTILE UNIV
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