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Method for preparing chiral deuterated methionine by using heavy water as deuterium source

A technology of deuterated methionine and methionine ester, which is applied in the field of preparation of chiral deuterated methionine, can solve the problems of complex synthesis steps, harsh synthesis conditions, failure to meet application value and market value, and achieve high atom economy and simple operation Effect

Inactive Publication Date: 2021-08-03
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the synthesis technology of deuterated methionine is not mature, and there are problems of complex synthesis steps or harsh synthesis conditions, which cannot meet its huge application value and market value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] In a water-free and oxygen-free glove box under an argon atmosphere, the Pd(OAc) 2 (0.001 mmol), ( R , R )-PhBox (0.0012 mmol), AgOTf (0.002 mmol), Fe (0.6 mmol) and α - Add dehydroamino acid ester (0.2 mmol) to a 10 mL reaction tube in turn, then add 2 ml DCE (1,2-dichloroethane), then add heavy water (0.4 mmol) and send it out of the glove box. Reaction in 60°C oil bath, TLC monitoring and I 2 Chromogenic detection reaction, after the reaction is completed, concentrate, and pass through the column with silica gel to obtain a white solid, namely chiral N-(tert-butoxycarbonyl)-methioninate, with a yield of 95%. ee The value is 98%, and the deuterium incorporation rate is 96%. 1 H NMR (400 MHz, CDCl 3 ): δH = 1.32 (9H, s), 1.82 (0.1H, dq), 1.93-2.06(4H, m), 2.42 (2H, t), 3.63 (3H, s), 4.29 (0.1H, m), 5.27 (1H, d). 13C NMR (100 MHz, CDCl3): δC = 15.3, 28.2, 29.9, 31.9, 52.2, 52.6, 79.7, 155.3, 172.7.

[0028] Add the above white solid to a round bottom flask, add ...

Embodiment 2

[0030] In a water-free and oxygen-free glove box under an argon atmosphere, [RhCl(COD)] 2 (0.002 mmol), ( R )-Seghos (0.0024 mmol), HB (C 6 f 5 ) 3 (0.01mmol), S 8 (0.1 mmol) and α - Dehydroamino acid esters (0.1 mmol) were sequentially added to a 10 mL reaction tube, then 2 ml tetrahydrofuran was added, and heavy water (0.1 mmol) was added and sent out of the glove box. Reaction in -50°C oil bath, TLC monitoring and I2 The reaction was detected by color development. After the reaction was completed, it was concentrated and passed through a column with silica gel to obtain a white solid. The yield is 20%, ee The value is 88%, and the deuterium incorporation rate is 85%. 1 H NMR (400 MHz, CDCl 3 ): δH = 1.32(9H, s), 1.82 (0.15H, dq), 1.93-2.06 (4H, m), 2.42 (2H, t), 3.63 (3H, s), 4.29 (0.15H, m), 5.27 (1H, d). 13C NMR (100 MHz, CDCl3): δC = 15.3, 28.2, 29.9, 31.9, 52.2, 52.6, 79.7, 155.3, 172.7.

[0031] Step 2: Add the above white solid to a round bottom flask, ad...

Embodiment 3

[0033] Step 1: Ni(OTf) 2 (0.01 mmol), ( R )-Binap (0.012 mmol), B (C 6 f 5 ) 3 (0.2 mmol), H 2 S (0.8mmol) and α - Dehydroamino acid esters (0.3 mmol) were sequentially added to a 10 mL reaction tube, then 2 ml tetrahydrofuran was added, and then water (0.8 mmol) was added and sent out of the glove box. Reaction in 80°C oil bath, TLC monitoring and I 2 The reaction was detected by color development. After the reaction was completed, it was concentrated and passed through a column with silica gel to obtain a white solid. Yield is 75%, ee The value is 88%, and the deuterium incorporation rate is 87%. 1 H NMR (400 MHz, CDCl 3 ): δH = 1.32 (9H,s), 1.82 (0.13H, dq), 1.93-2.06 (4H, m), 2.42 (2H, t), 3.63 (3H, s), 4.29(0.13H, m), 5.27 (1H, d). 13C NMR (100 MHz, CDCl3): δC = 15.3, 28.2, 29.9, 31.9, 52.2, 52.6, 79.7, 155.3, 172.7.

[0034] Step 2: Add the above white solid to a round bottom flask, add a mixed solvent of TFA (1.8 mL) and dichloromethane (2 mL), stir at room...

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Abstract

The invention discloses a method for preparing chiral deuterated methionine by taking heavy water as a deuterium source. The method comprises the following steps of: with N-(t-butyloxycarbonyl)-dehydromethionine ester and heavy water as reaction raw materials and transition metal, lewis acid and chiral ligand as catalysts, carrying out a reduction reaction in an organic solvent under the action of a reducing agent to synthesize chiral N-(t-butyloxycarbonyl)-methionine ester; and finally, carrying out acid deprotection to obtain chiral deuterated methionine. According to the method, chiral deuterated methionine is prepared through asymmetric hydrogenation reaction of alpha-dehydromethionine ester by adopting a metal / Lewis acid coordinated catalysis system and taking the heavy water as a unique deuterium source; and compared with an existing common heavy water exchange method, a reaction temperature can be reduced to 60 DEG C from 150 DEG C, the amount of heavy water only needs to be 200% of the molar percentage of a dehydroamino acid ester compound, an atmosphere with heavy water as a solvent is not needed, and conditions are milder. Compared with a traditional deuterium source, the method has the advantages that operation is simple, atom economy is high, and the deuterium doping rate and enantioselectivity of the chiral deuterated methionine are high by taking the heavy water as the deuterium source.

Description

technical field [0001] The invention belongs to the technical field of chemical synthesis, and in particular relates to a method for preparing chiral deuterated methionine by using heavy water as a deuterium source. Background technique [0002] Methionine is one of the essential amino acids that constitute the human body and participates in protein synthesis. For the human body, methionine has very important functions: 1. It is one of the main components of amino acid infusion and compound amino acid. 2. Methionine can be used to synthesize medicinal vitamins, nutritional supplements, and feed additives. 3. Methionine is one of the eight essential amino acids for the human body and participates in the metabolism of life. Methionine cannot be produced in the body itself and must be obtained from the outside world. Therefore, the synthesis and production of methionine has great application value and prospect. The preparation of deuterated methionine can be used for the ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C319/20C07C323/59C07C323/58C07B59/00
CPCC07B59/001C07B2200/05C07B2200/07C07C319/20C07C323/59C07C323/58
Inventor 陈景超樊保敏周永云潘海丽徐建斌和振秀樊瑞峰孙蔚青刘浩杰
Owner YUNNAN MINZU UNIV