Preparation method of bacteroides fragilis capsular polysaccharide A

A technology of Bacteroides fragilis and capsular polysaccharides, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of toxic and harmful organic solvents, unfavorable production safety and environmental protection, etc., to promote shedding, gain The effect of increasing the rate and reducing the toxicity
CN113234770APending Publication Date: 2021-08-10GUANGZHOU ZHIYI PHARMA INC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU ZHIYI PHARMA INC
Publication Date
2021-08-10

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Abstract

The invention relates to a preparation method of bacteroides fragilis capsular polysaccharide A. The preparation method comprises the following steps: preparing a bacterial suspension of bacteroides fragilis with the pH value of less than or equal to 5, heating and extracting, and collecting an extracting solution to obtain a crude sugar solution of capsular polysaccharide A. Compared with the prior art, the preparation method has the following beneficial effects that the inventor accidentally finds that the capsular polysaccharide A can be effectively promoted to fall off from the bacteroides fragilis thalli by heating and extracting the bacteroides fragilis bacterial suspension of which the pH value is less than or equal to 5, so that the capsular polysaccharide A is extracted. Compared with a traditional phenol / water method, the capsular polysaccharide A can be obtained under the condition that phenol is not used, and the toxicity is low. Moreover, by adopting the preparation method, the yield of the bacteroides fragilis capsular polysaccharide A is obviously improved.
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Description

technical field

[0001] The invention relates to the preparation and application technology of Bacteroides fragilis capsular polysaccharide, in particular to a preparation method of Bacteroides fragilis capsular polysaccharide A. Background technique

[0002] Bacteroides fragilis (bacteroides fragilis) is a member of the genus Bacteroides among Gram-negative anaerobic bacteria, belonging to the phylum Bacteroidetes, which is completely different from bifidobacteria and lactic acid bacteria of the phylum Firmicutes. There are 25 species of Bacteroides, 10 species from humans only, 10 species from animals only, and 5 species from both humans and animals. Bacteroides fragilis is an obligate anaerobic bacterium, which can be divided into enterotoxigenic Bacteroides fragilis (ETBF)) and non-enterotoxigenic Bacteroides fragilis (ETBF) according to whether it can synthesize and secrete Bacteroides fragilis enterotoxin (BFT) Toxin-type Bacteroides fragilis (Nontoxigenic Bacteroides ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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