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Dual-emission gold cluster ratiometric fluorescent probe and preparation method thereof, and doxycycline detection method

A ratiometric fluorescent probe, doxycycline technology, applied in the field of biosensing, to achieve the effect of good selectivity, wide application range and high sensitivity

Active Publication Date: 2021-08-24
四川中科微纳科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no studies have reported BSA-Au NCs with dual emission peaks as ratiometric fluorescent probes in analytical sensing.

Method used

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  • Dual-emission gold cluster ratiometric fluorescent probe and preparation method thereof, and doxycycline detection method
  • Dual-emission gold cluster ratiometric fluorescent probe and preparation method thereof, and doxycycline detection method
  • Dual-emission gold cluster ratiometric fluorescent probe and preparation method thereof, and doxycycline detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation method of dual-emission gold cluster ratio fluorescent probe comprises the following steps:

[0059] (1) Mix 5mL, 10mM, 37°C HAuCl4·4H under vigorous stirring 2 Add O solution to 5mL, 50mg / mL, bovine serum albumin solution at 37°C;

[0060] (2) After 2 minutes, add 1M NaOH solution dropwise to adjust the pH of the mixture to 12;

[0061] (3) Stirring was continued for 12 h under the condition of 37°C water bath, and finally the product BSA-Au NCs was obtained.

Embodiment 2

[0063] The dual-emission gold cluster ratio fluorescent probe prepared in Example 1 was characterized by HRTEM, FT-IR and XPS ( Figure 1-4 ), the results show that the morphology of the dual-emission gold cluster ratio fluorescent probe under the high-resolution transmission electron microscope is a uniformly distributed monodisperse quasi-spherical shape, with a diameter of 0.5-3.5nm and an average value of 2.06nm. Through FT-IR and XPS characterization, it can be seen that bovine serum albumin, as a protective agent and reducing agent, successfully synthesized gold ions into gold clusters.

Embodiment 3

[0065] Scan the ultraviolet spectrum of the dual-emission gold cluster ratio fluorescent probe prepared in Example 1, the fluorescence optimal excitation spectrum and the corresponding fluorescence emission spectrum under the optimal excitation wavelength ( Figure 5 ). Such as Figure 5 As shown, when the excitation wavelength is 400 nm, the BSA-Au NCs show dual emission peaks at 480 nm and 640 nm.

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Abstract

The invention relates to a dual-emission gold cluster ratiometric fluorescent probe and a preparation method thereof, and a doxycycline detection method, wherein the dual-emission gold cluster material is prepared by using bovine serum albumin to modify a gold cluster, and the dual-emission gold cluster material has fluorescence emission peaks at 480 nm and 640 nm under excitation of 390 nm. The double-emission gold cluster material is applied to high-selectivity and high-sensitivity detection of doxycycline as a ratiometric fluorescent probe. The invention also discloses an application of the double-emission gold cluster material in selective detection of doxycycline. The detection method can effectively eliminate system errors and has the advantages of being low in detection limit, good in accuracy, simple and rapid in detection and the like.

Description

technical field [0001] The invention relates to the technical field of biosensing, in particular to a dual-emission ratio fluorescent probe and a preparation method thereof, and a method for using the fluorescent probe to detect doxycycline in food. Background technique [0002] In recent years, antibiotics have been widely used in agriculture, animal husbandry, aquaculture and pharmaceutical industries. As an emerging pollutant, once excessive residues, it will cause potential risks to soil, water and food quality and safety, and then ecologically Circulation and other ways further endanger human health and the ecological environment. Since antibiotics were approved as animal feed additives, the production and usage of antibiotics have increased rapidly around the world. Doxycycline is a semi-synthetic tetracycline antibiotic produced by deoxygenating the 6-position of oxytetracycline. According to reports, in 2018, the veterinary use of tetracycline antibiotics in my cou...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/58B82Y20/00B82Y30/00B82Y40/00C09K11/06G01N21/64
CPCC09K11/02C09K11/58B82Y20/00B82Y30/00B82Y40/00C09K11/06G01N21/64G01N21/6428
Inventor 霍峰蒋志王曦妮王显祥陈银银
Owner 四川中科微纳科技有限公司
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