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Application of GLUT1 as prevention and/or treatment target of hyperpigmented villus nodular synovitis

A pigmentation and treatment target technology, applied in the field of biomedicine, can solve the problems of patients' life and psychological impact, high recurrence rate, and only re-surgical resection.

Pending Publication Date: 2021-08-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pigmented villonodular synovitis is divided into localized, diffuse, and intermediate types. Currently, pigmented villonodular synovitis is mainly surgical resection, supplemented by radiotherapy and chemotherapy. However, the specificity of chemotherapy drugs is only 30%-50%. At the same time, radiotherapy has a greater impact on patients at the age of onset of the disease, and the postoperative recurrence rate of pigmented villonodular synovitis is relatively high. Can be surgically removed again, which has a huge impact on the life and psychology of the patient

Method used

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  • Application of GLUT1 as prevention and/or treatment target of hyperpigmented villus nodular synovitis
  • Application of GLUT1 as prevention and/or treatment target of hyperpigmented villus nodular synovitis
  • Application of GLUT1 as prevention and/or treatment target of hyperpigmented villus nodular synovitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] In this example, the morphology comparison of synoviocytes in pigmented villonodular synovitis and normal synoviocytes was carried out.

[0051] 1. Test material

[0052] Synovial specimens of the knee joint.

[0053] 2. Test method

[0054] Take a normal knee joint synovial specimen and a knee joint synovial specimen from a patient with pigmented villonodular synovitis, and perform the following treatment: wash the knee joint synovial specimen twice with PBS containing double antibodies and soak 15min. The synovial tissue was taken out, the fat was removed, shredded and digested in 0.25% trypsin for 30 minutes, shaken during the period, centrifuged at 1000r / min for 5 minutes, and the trypsin was discarded. Then it was digested with 0.2% collagenase II (prepared with DMEM containing 10% serum and double antibody, that is, complete medium) for 2 hours, and shaken during the period. Similarly, the collagenase II was discarded after centrifugation. Add about 1 mL of co...

Embodiment 2

[0058] This embodiment detects the expression of TXNIP and GLUT1 in pigmented villonodular synovitis synoviocytes and normal synoviocytes.

[0059] 1. Test material

[0060] Synoviocytes in pigmented villonodular synovitis versus normal synoviocytes.

[0061] 2. Test method

[0062] This example compares the expression of TXNIP and GLUT1 in normal synovium and pigmented villonodular synovitis synoviocytes. The cells are treated with IL-1β for 24 hours to simulate the inflammatory environment, and the pigmented villonodular synovitis is detected by WB and qPCR. Expression levels of TXNIP and GLUT1 in meningitis synoviocytes and normal synoviocytes.

[0063] 3. Test results

[0064] See the result figure 2 , figure 2 It is the expression level figure of TXNIP and GLUT1 in synoviocytes of the present invention, wherein, figure 2 A is the qpcr detection of TXNIP and GLUT1 mRNA expression in synoviocytes, figure 2 B is the protein expression map of TXNIP and inflammatory...

Embodiment 3

[0066] In this example, the detection of glycolysis levels, aerobic phosphorylation levels and related protein expression levels in pigmented villonodular synovitis synoviocytes and normal synoviocytes was carried out.

[0067] 1. Test material

[0068] Synoviocytes in pigmented villonodular synovitis versus normal synoviocytes.

[0069] 2. Test method

[0070]This example analyzes the functional mode of synoviocytes in pigmented villonodular synovitis, on the one hand, compares the glycolysis and aerobic phosphorylation of normal synoviocytes and pigmented villonodular synovitis synoviocytes At the same time, the cells were treated with IL-1β for 24 hours to simulate the inflammatory environment, and the changes of the above two kinds of energy supply were further detected. ECAR is an extracellular acidification rate test, through which changes in glycolysis are analyzed, and oxygen consumption is used to detect changes in aerobic phosphorylation levels. Cellular oxygen co...

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Abstract

The invention relates to the technical field of biological medicine, and concretely relates to application of GLUT1 as a prevention and / or treatment target of hyperpigmentation villus nodular synovitis. The applicant discovers that the GLUT1 is highly expressed in the synovial cells of the hyperpigmented villus nodular synovitis for the first time, the invention provides the application of the GLUT1 as the prevention and / or treatment target of the hyperpigmented villus nodular synovitis, and the GLUT1 can be used as a drug target for screening drugs for preventing and / or treating the hyperpigmented villus nodular synovitis, and a novel screening method and a novel research and development thought are provided for the drugs for preventing and / or treating hyperpigmentation villus nodular synovitis.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of GLUT1 as a target for the prevention and / or treatment of pigmented villonodular synovitis. Background technique [0002] Pigmented villonodular synovitis is characterized by reactive inflammation and neoplasticity, and is prone to occur in various joints. It is a joint disease mainly manifested by joint pain such as reactive synovial hyperplasia, vascular hyperplasia, and joint osteochondral destruction. . Pigmented villonodular synovitis is divided into localized, diffuse, and intermediate types. Currently, pigmented villonodular synovitis is mainly surgical resection, supplemented by radiotherapy and chemotherapy. However, the specificity of chemotherapy drugs is only 30%-50%. At the same time, radiotherapy has a greater impact on patients at the age of onset of the disease, and the postoperative recurrence rate of pigmented villonodular synovitis is rel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61K39/395A61K31/7088A61P19/02A61P29/00
CPCA61K31/7088A61K39/3955A61K45/00A61P19/02A61P29/00C12Q1/6883C12Q2600/136C12Q2600/158G01N33/6893G01N2333/47G01N2800/102
Inventor 张还添杨杰汤旺赖毓霄黎贞燕张灵敏查振刚
Owner JINAN UNIVERSITY
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