Method for producing malic acid

A technology of malic acid and seeds, which is applied in the biological field, can solve the problems of less fermentation liquid, slow processing speed, and high cost, and achieve the effects of high yield and purity, simple operation process, and low production cost

Active Publication Date: 2021-08-31
SHANDONG FUFENG FERMENTATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the amount of fermented liquid concentrated by the ultrafiltration membrane is small, the p

Method used

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  • Method for producing malic acid

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Embodiment 1

[0022] A method for producing malic acid, comprising the steps of:

[0023] Aspergillus oryzae ACCC30584 bacteria solution (concentration of 1 × 10 8 CFU / mL) according to the inoculum amount of 10% (volume ratio) into the seed tank for cultivation, at a temperature of 33 ° C, a shaker speed of 200 r / min, and culturing for 12 hours to obtain a seed liquid, wherein the medium group of the seed tank Divided into (1L): 3g sucrose, 0.2g sodium nitrate, 0.5g ammonium sulfate, 0.1g potassium dihydrogen phosphate, 0.1g dipotassium hydrogen phosphate, 0.1g manganese sulfate monohydrate, 0.01g ferrous sulfate heptahydrate, magnesium sulfate 0.01g, sodium chloride 0.01g, pH 6.0;

[0024] According to the volume ratio of seed liquid: fermenter culture medium is 1: 10, transfer in the fermenter to cultivate, temperature 33 ℃, cultivate 48h, then add calcium carbonate and natamycin, the addition of calcium carbonate is 80g / L, Natamycin The added amount of tamycin is 5 mg / L, and the fermen...

Embodiment 2

[0028] A method for producing malic acid, comprising the steps of:

[0029] Aspergillus oryzae ACCC30584 bacteria solution (concentration of 1 × 10 8 CFU / mL) according to the inoculum amount of 10% (volume ratio) into the seed tank for cultivation, at a temperature of 33 ° C, a shaker speed of 200 r / min, and culturing for 12 hours to obtain a seed liquid, wherein the medium group of the seed tank Divided into (1L): 3g sucrose, 0.2g sodium nitrate, 0.5g ammonium sulfate, 0.1g potassium dihydrogen phosphate, 0.1g dipotassium hydrogen phosphate, 0.1g manganese sulfate monohydrate, 0.01g ferrous sulfate heptahydrate, magnesium sulfate 0.01g, sodium chloride 0.01g, pH 6.0;

[0030] According to the volume ratio of seed liquid: the fermenter medium is 1: 10, transfer it to the fermenter for cultivation, temperature is 33 ℃, cultivate 48h, then add calcium carbonate, the addition of calcium carbonate is 80g / L, continue to ferment for 36h, then control The temperature is 39°C, the p...

Embodiment 3

[0034] A method for producing malic acid, comprising the steps of:

[0035] Aspergillus oryzae ACCC30584 bacteria solution (concentration of 1 × 10 8CFU / mL) according to the inoculum amount of 10% (volume ratio) into the seed tank for cultivation, at a temperature of 33 ° C, a shaker speed of 200 r / min, and culturing for 12 hours to obtain a seed liquid, wherein the medium group of the seed tank Divided into (1L): 3g sucrose, 0.2g sodium nitrate, 0.5g ammonium sulfate, 0.1g potassium dihydrogen phosphate, 0.1g dipotassium hydrogen phosphate, 0.1g manganese sulfate monohydrate, 0.01g ferrous sulfate heptahydrate, magnesium sulfate 0.01g, sodium chloride 0.01g, pH 6.0;

[0036] According to the volume ratio of seed liquid: fermenter culture medium is 1: 10, transfer in the fermenter to cultivate, temperature 33 ℃, cultivate 48h, then add calcium carbonate and natamycin, the addition of calcium carbonate is 80g / L, Natamycin The added amount of tamycin is 5 mg / L, and the ferment...

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Abstract

The invention belongs to the technical field of biology, and discloses a method for producing malic acid. The method comprises the following steps: taking malic acid fermentation liquor, centrifuging to remove precipitate, collecting supernatant, adding activated carbon, stirring, adding diatomite, uniformly stirring, filtering by a plate frame, collecting filtrate, filtering by a micro-filtration membrane, collecting micro filtrate, absorbing malic acid through a chromatographic column filled with strong-base and weak-acid type amphoteric ion exchange resin, then eluting the malic acid adsorbed on the resin with hot water, performing elution repeatedly, mixing obtained eluates, and carrying out reduced pressure distillation and vacuum drying to obtain the malic acid product.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing malic acid. Background technique [0002] L-malic acid is widely used in the fields of food, medicine, and daily chemical industry. Its preparation methods include biological fermentation and chemical synthesis. The biological fermentation method is green and environmentally friendly, and has become a trend in the preparation of malic acid. [0003] The production of L-malic acid is mainly based on biological fermentation. How to improve the efficiency of biological fermentation is a technical problem that researchers have always needed to solve. Aiming at this technical problem, the applicant has done a lot of research, for example as follows: CN108300739B, a kind of method for the separation of L-malic acid, adopts the mode of Aspergillus mixed fermentation culture, compares conventional Aspergillus oryzae fermentation method, can improve apple T...

Claims

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Application Information

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IPC IPC(8): C07C51/42C07C51/47C07C59/245C12P7/46C12R1/69
CPCC07C51/42C07C51/47C12P7/46C07C59/245
Inventor 刘建阳来凤堂薄文文袁军郑世涛郝武斌刘晶晶
Owner SHANDONG FUFENG FERMENTATION CO LTD
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