Three kinds of alkaloid compounds in purslane and their extraction and separation methods

A technology of alkaloids and separation methods, applied in the field of three alkaloid compounds and their extraction and separation, can solve the problem of low structural novelty, and achieve the effect of environmentally friendly process methods, simple and fast operation methods

Active Publication Date: 2022-05-20
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the chemical components isolated from purslane are known, and the structural novelty is low. Therefore, the development and isolation of compounds in purslane are urgently needed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three kinds of alkaloid compounds in purslane and their extraction and separation methods
  • Three kinds of alkaloid compounds in purslane and their extraction and separation methods
  • Three kinds of alkaloid compounds in purslane and their extraction and separation methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The present invention provides three kinds of alkaloid compounds, molecular formula is C 16 h 15 NO 5 (1), C 13 h 11 NO 3 (2), C 14 h 13 NO 3 (3), and named according to its structure 4,9,10-trihydroxy-2-methoxy-6,7-dihydrodibenzo[b,e]azocin-12(5H)-one(1), 9,10-dihydroxy- 5,6-dihydro-11H-benzo[d]pyrrolo[1,2-a]azepin-11-one (2), 8,9-dihydroxy-6,11-dihydro-5H-benzo[d]pyrrolo[1 ,2-a]azepine-3-carbaldehyde (3), the chemical structural formula is:

[0060] .

[0061] The NMR data of the three alkaloid compounds are shown in Table 1: 1 H-NMR with 13 C-NMR in MeOD.

[0062] Table 1: NMR data of three alkaloid compounds of the present invention

[0063] .

[0064] The alkaloid compound 1 of the present invention is named 4,9,10-trihydroxy-2-methoxy-6,7-dihydrodibenzo[b,e]azocin-12(5H)-one according to its structure identification and derivation, Table 1 is NMR data of the alkaloid compound.

[0065] 4,9,10-trihydroxy-2-methoxy-6,7-dihydrodibenzo[b,e]azocin-1...

Embodiment 2

[0079] Example 2 Anti-inflammatory effects of three alkaloid compounds of the present invention.

[0080] 1. Main materials.

[0081] 1.1. Drugs and reagents: The compounds used in the experiment were prepared by the above method with a purity of ≥98%, weighed accurately, and diluted with DMSO to the required solutions for the following dosage groups. Fetal bovine serum (U.S. Hyclone Company); penicillin and streptomycin (Hangzhou Sijiqing Company); DMSO (U.S. Sigma Company); CCK-8 kit (Wuhan Boster Bioengineering Co., Ltd.); DMEM high glucose medium, LPS , IL-1 β , TNF- α ELISA kit (Soleibao Technology Co., Ltd.); cell lysate.

[0082] 1.2 Cell line: RAW264.7 macrophages (US ATCC cell bank).

[0083] 1.3 Grouping: control group, LPS group and experimental group, each group.

[0084] 2 Experimental methods.

[0085] 2.1 Cell culture, DMEM high-glucose medium, add 10% fetal bovine serum, 1% antibiotics (100U / mL penicillin and 100μg / mL streptomycin), place at 37°C, 5% CO ...

Embodiment 3

[0098] Example 3 Anticholinesterase effect of three compounds of the present invention.

[0099] 1. Main materials.

[0100] Drugs and reagents: The alkaloid compounds used in the experiment were prepared by the above method with a purity of ≥98%, sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), phosphorus 5 , 5'-dithiobisnitrobenzoic acid (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and iodide thioacetylcholine (Acetylthiocholine iodide, ATCI, Dalian Meilun Biotechnology Co., Ltd.).

[0101] 2. Grouping: divided into blank group, control group and sample group.

[0102] 3 Experimental methods.

[0103] 3.1 Sample preparation

[0104] Precisely weigh the sample and 0.11 mg of physostigmine, respectively, and use methanol as a solvent to prepare five gradient concentrations of 2.5 μM, 5.0 μM, 10.0 μM, 20.0 μM and 40.0 μM. Accur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
degree of unsaturationaaaaaaaaaa
degree of unsaturationaaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to three alkaloid compounds extracted, separated and identified from purslane and an extraction and separation method thereof. Described three kinds of alkaloid compounds, molecular formula is respectively C 16 h 15 NO 5 , C 13 h 11 NO 3 , C 14 h 13 NO 3 , named as 4,9,10-trihydroxy-2-methoxy-6,7-dihydrodibenzo[b,e]azocin-12(5H)-one, 9,10-dihydroxy-5,6-dihydro-11H-benzo [d]pyrrolo[1,2‑a]azepin‑11‑one, 8,9‑dihydroxy‑6,11‑dihydro‑5H‑benzo[d]pyrrolo[1,2‑a]azepine‑3‑carbaldehyde. It also provides an extraction and separation method for the above-mentioned alkaloid compounds, which is sequentially prepared by ethanol reflux extraction, silica gel column chromatography, ODS medium pressure column and hydroxypropyl dextran column chromatography, and HPLC separation and preparation. Its structure adopts 1 H-NMR, 13 C-NMR and UHPLC-ESI-TOF-MS methods identified three new alkaloid compounds. The three compounds have potential anti-inflammatory and anti-cholinesterase activities, and provide preparation methods to provide lead and theoretical basis for the development of new drugs and new ingredients.

Description

technical field [0001] The invention relates to the field of extraction and separation of traditional Chinese medicines, in particular to three alkaloid compounds extracted, separated and identified from purslane medicinal material and an extraction and separation method thereof. Background technique [0002] purslane( Portulaca oleracea L.), also known as long-lived vegetable and horse amaranth, is a plant of the family Portulaca oleracea. Purslane likes fertile soil, is resistant to drought and waterlogging, has strong vitality, is widely distributed, and is rich in resources, and it is more common in Northeast my country. Purslane can be used as both medicine and food, and it is one of the wild plants with the same origin of medicine and food designated by the Ministry of Health of my country. The 2020 edition of "The Pharmacopoeia of the People's Republic of China" records that the dried aerial part of purslane is used as medicine. It has the effects of clearing away ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07D225/08C07D487/04A61P29/00A61P43/00
CPCC07D225/08C07D487/04A61P29/00A61P43/00Y02A50/30
Inventor 英锡相崔馨予英哲铭兰秀娟
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap