Preparation method of self-assembled fluorescent probe for detecting nitrite, fluorescent probe prepared by preparation method and application of fluorescent probe
A nitrite, fluorescent probe technology, applied in the field of fluorescent probes, can solve the problems of low biocompatibility, poor solubility, complex synthesis, etc., and achieve the effects of high sensitivity, low cost, and simple synthesis
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Embodiment 1
[0065] This embodiment is a preparation method for measuring nitrite self-assembled fluorescent probe, comprising the following steps:
[0066] S1. Prepare gold nanoparticles with different particle sizes, use fluorescence spectrum, start at 200nm, and continuously change the wavelength of excitation light with an increase rate of 3-8nm until the peak of the highest emission peak no longer rises. Take a 1cm fluorescent cuvette, Use a pipette gun to take 2 mL of nano-gold into a cuvette. Scan with a fluorescence spectrometer to observe its fluorescence spectrum;
[0067] S2. Take a 1cm fluorescence cuvette, take 2mL of nano-gold in the cuvette with a pipette gun, use the highest peak of the emission peak measured in step S1 as the emission wavelength, observe its fluorescence spectrum, and measure the excitation wavelength of nano-gold;
[0068] S3. Take the prepared nano-gold with a particle size of 16.4nm, use a fluorescence spectrometer to scan at a wavelength of 400-600nm,...
Embodiment 2
[0094] Weigh 5g of a certain brand of ham sausage purchased from a supermarket, mix it evenly, put it in a beaker, and add 12.5mL of saturated borax solution. Wash the crushed and mixed ham sausage with water at about 65°C into a 500mL volumetric flask. Heat in a boiling water bath for 15 minutes, cool to about 20°C, add 5mL of 106g / L potassium ferrocyanide solution, mix well, then add 5mL of 220g / L zinc acetate solution. Store sealed in the refrigerator for later use.
[0095] Add different concentrations of sodium nitrite standard solution to the prepared ham sample solution to determine the recovery and precision of the detection system for different concentrations of sodium nitrite in the sample. Each group of experiments was repeated 3 times. The results are shown in Table 1.
[0096] Table 1 Standard recovery experiment of nitrite content in ham sausage
[0097]
[0098] The results showed that the standard addition recovery rate was between 96.39% and 102.12%, and...
Embodiment 3
[0100] After stirring a certain brand of mustard greens purchased through a supermarket into a paste in a juice extractor, take 100g of mustard mustard paste and put it in a beaker, and add 6mL of saturated borax solution. Wash the mustard paste into a 500mL volumetric flask with water at about 65°C. Add 2g of activated carbon powder to the volumetric flask, then add 2mL of 106g / L potassium ferrocyanide solution, mix well and add 2mL of 220g / L zinc acetate solution. Store sealed in the refrigerator for later use.
[0101]Sodium nitrite standard solution of different concentrations was added to the prepared pickled mustard sample solution to determine the recovery rate and precision of this method for detecting nitrite. Each group of experiments was repeated three times. The results are shown in Table 2.
[0102] Table 2 The standard addition recovery experiment of nitrite content in mustard mustard
[0103]
[0104] The results showed that the standard addition recovery r...
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