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Rapid detection method for illegally added pioglitazone in health food

A technology for illegal addition of pioglitazone, which is applied in the field of rapid detection of illegal addition of pioglitazone, can solve the problems of low sensitivity and achieve the effects of low cost, reduced usage, and high speed

Pending Publication Date: 2021-09-03
河北省食品检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surface-enhanced Raman spectroscopy (SERS) uses trace molecules to adsorb on Cu, Ag, Au and other metal sols and electrode surfaces, and its Raman spectrum signal can be enhanced by 10 4 ~10 6 , overcoming the shortcomings of low sensitivity of conventional Raman spectroscopy

Method used

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  • Rapid detection method for illegally added pioglitazone in health food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Rapid qualitative analysis of illegally added pioglitazone in health food

[0050] Sample: commercially available capsule hypoglycemic health food.

[0051] Preprocessing:

[0052] a. Take the contents of the capsule equivalent to one oral dose, add 2 mL of anhydrous methanol for oscillation, and place it in an ultrasonic extractor for 10 minutes of ultrasonication.

[0053] b. Shake the extract and filter.

[0054] Qualitative analysis:

[0055] Take 100 μL sample filtrate, add 400 μL gold nanosilver enhancer, add 100 μL acidifier, shake well and test, according to 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm-1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0056] If the liquid to be tested is at 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm -1 (±3cm -1 ) place has a characteristic peak, then it shows that the sample contains pioglitazone.

Embodiment 2

[0057] Example 2 Rapid qualitative analysis of illegally added pioglitazone in health food

[0058] Sample: commercially available tablet or granule hypoglycemic health food.

[0059] Preprocessing:

[0060] a. Take a tablet or granule equivalent to one oral dose, grind it finely, add 2 mL of anhydrous methanol for oscillation, and place it in an ultrasonic extractor for 10 minutes of ultrasonication.

[0061] b. Shake the extract and filter.

[0062] Qualitative analysis:

[0063] Take 100 μL sample filtrate, add 400 μL gold nanosilver enhancer, add 100 μL acidifier, shake well and test, according to 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm -1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0064] If the liquid to be tested is at 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm -1 (±3cm -1 ) place has a characteristic peak, then it shows that the sample contains pioglitazone.

Embodiment 3

[0065] Example 3 Rapid qualitative analysis of illegally added pioglitazone in health food

[0066] Sample: commercially available liquid hypoglycemic health food.

[0067] Qualitative analysis:

[0068] The sample does not need to be pretreated, take 100 μL sample, add 400 μL gold nanosilver enhancer, add 100 μL acidifier, shake well and detect, according to 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm -1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0069] If the liquid to be tested is at 820cm -1 (±3cm -1 ), 1180cm -1 (±3cm -1 ), 1210cm -1 (±3cm -1 ) place has a characteristic peak, then it shows that the sample contains pioglitazone.

[0070] The present invention adopts special-purpose software solidified in a portable Raman spectrometer to realize an ultra-fast, low-cost, and on-site identification method for illegally adding pioglitazone to health food. The detection time of a sample is less than 15 minutes, the operation is s...

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Abstract

The invention discloses a rapid detection method for illegally added pioglitazone in health food. The rapid detection method comprises the following steps: (1) preparing a standard substance to-be-detected solution; (2) sample pretreatment: (21) weighing a proper amount of sample, adding absolute methanol, carrying out oscillating, and carrying out ultrasonic treatment in an ultrasonic extraction instrument for 10 minutes to obtain an extracting solution; (22) uniformly shaking and filtering the extracting solution; and (3) carrying out surface enhanced laser Raman spectrum analysis: taking a proper amount of filtrate as a to-be-detected solution, sequentially adding a surface enhanced reagent and an acidifying agent, and recording whether 820cm <-1 > (+ / -3cm <-1 >), 1180cm <-1 > (+ / -3cm <-1 >) and 1210cm <-1 > (+ / -3cm <-1 >) have characteristic peaks or not. The method disclosed by the invention is simple and convenient, low in cost, beneficial to field operation and capable of rapidly detecting results; qualitative analysis of a large number of samples can be realized; the usage amount of chemical reagents is reduced, and harm to operators and pollution to the environment are reduced; and the speed is fast, the cost is low and operation is easy.

Description

technical field [0001] The invention relates to the technical field of health product detection, in particular to a rapid detection method for illegally added pioglitazone in health food. Background technique [0002] Pioglitazone is a thiazolidinedione antidiabetic drug, which is an insulin sensitizer. The mechanism of action is related to the presence of insulin. It can reduce insulin resistance in peripheral tissues and liver, increase insulin-dependent glucose processing, and reduce glycogen output. The main adverse reactions of pioglitazone are edema, headache, dizziness, nausea, rash, hypoglycemia reaction, etc. Severe adverse reactions are manifested as: heart failure, severe abnormal liver function, hypoglycemia coma, etc. In November 2011, the National Food and Drug Administration issued an information report on adverse drug reactions, suggesting that pioglitazone can cause the risk of bladder cancer. As a special food, health food has the function of regulating t...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/34
CPCG01N21/658G01N1/34
Inventor 张兰天郭美娟史国华张岩王一玮吴昊刘晓光
Owner 河北省食品检验研究院
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