A cuni/comoo as a multifunctional laccase-like 4 Preparation method and application of

A multi-functional, laccase technology, applied in chemical instruments and methods, water/sludge/sewage treatment, chemical analysis by catalysis, etc., to achieve good substrate versatility, efficient degradation, and increase the effect of specific surface area

Active Publication Date: 2022-08-05
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, it remains a great challenge to prepare multifunctional nanozymes with high laccase-like activity for chemical biosensing, pollutant degradation, and activation of PMS, etc.

Method used

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  • A cuni/comoo as a multifunctional laccase-like <sub>4</sub> Preparation method and application of
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  • A cuni/comoo as a multifunctional laccase-like <sub>4</sub> Preparation method and application of

Examples

Experimental program
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Effect test

preparation example Construction

[0075] A multifunctional laccase-like CuNi / CoMoO 4 The preparation method comprises the following steps:

[0076] S1: CoMoO 4 preparation

[0077] will Na 2 MoO 4 ·2H 2 O and Co(NO 3 ) 2 ·6H 2 O was fully stirred and dissolved in deionized water, and then reacted in an autoclave; after centrifugation, washed three times with deionized water and ethanol to obtain the precursor; then vacuum-dried, and the precursor was calcined to obtain CoMoO 4 ;

[0078] S2: CuNi / CoMoO 4 preparation

[0079] the CuCl 2 ·2H 2 O, NiCl 2 ·6H 2 O and as-prepared CoMoO 4 Dissolve in deionized water and stir; then add NaHB 4 stirring, followed by washing with deionized water and ethanol, followed by vacuum drying to prepare CuNi / CoMoO 4 .

[0080] In step S1: Na 2 MoO 4 ·2H 2 O and Co(NO 3 ) 2 ·6H 2 The dosage ratio of O is the molar ratio, and the molar ratio is 1:1; the reaction conditions in the autoclave are: the reaction temperature is 155-165 °C, the reaction time is 9-...

Embodiment

[0090] (1) CuNi / CoMoO 4 Preparation example

[0091] S1: CoMoO 4 preparation

[0092] 1 mmol Na 2 MoO4 2H 2 O and 1mmolCo(NO 3 ) 2 ·6H 2 O was fully stirred and dissolved in 40 mL of deionized water, and then reacted in a 50 mL autoclave at 160 °C for 10 h; after centrifugation, washed three times with deionized water and ethanol to obtain the precursor; then vacuum-dried at 80 °C, the precursor was Calcined in air at 350 °C for 2 h to obtain CoMoO 4 .

[0093] S2: CuNi / CoMoO 4 preparation

[0094] Weigh 0.0768g of CuCl in a molar ratio of 3:1 2 ·2H 2 O, 0.0387g NiCl 2 ·6H 2 O and S1 prepared 0.04g CoMoO 4 Dissolve in 20mL deionized water and stir for 3h, then add 10mL, 10mg / mL NaHB 4 Stir for 5 h, then wash with deionized water and ethanol, and vacuum dry at 80 °C to obtain CuNi / CoMoO with a Cu / Ni molar ratio of about 3:1 4 .

[0095] like Figure 1 to Figure 4 As shown, CuNi / CoMoO was prepared by a combination of hydrothermal, calcination and impregnation ...

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Abstract

The present invention relates to a kind of CuNi / CoMoO as a multifunctional laccase-like 4 The preparation method and application, comprising the following steps: S1: CoMoO 4 Preparation and S2: CuNi / CoMoO 4 preparation. The present invention adopts the combined method of hydrothermal, calcination and impregnation to prepare CuNi / CoMoO 4 It is beneficial to the adsorption of reactants to participate in the enzymatic catalytic reaction, showing good catalytic activity. Utilize CuNi / CoMoO 4 The laccase-like activity of the ascorbic acid colorimetric sensor was established, a portable smart terminal platform combined with colorimetric signals was constructed, and the efficient degradation of acid fuchsin was achieved.

Description

technical field [0001] The invention relates to a technology for preparing and measuring chemical substances, in particular to a CuNi / CoMoO as a multifunctional laccase-like enzyme 4 preparation method and application. Background technique [0002] Nanomaterials with bioenzyme-like activity are called nanozymes, which have attracted great attention and interest in various fields because they can overcome the inherent shortcomings of natural enzymes, such as long fermentation time, low yield, and poor stability. Currently, many nanozymes have been demonstrated to possess peroxidase, oxidase, catalase, superoxide dismutase, and laccase-like properties, and have broad applications in biosensing, pollutant degradation, and environmental remediation. application prospects. For example, someone has successfully prepared MMoO 4 (M=Co, Ni) nanoflowers, which have peroxidase-like activity and can be used for copper ions (Cu 2+ ) selective detection. Others have prepared metal-or...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J23/885C02F1/72G01N21/78G01N21/31G01N21/33G01N31/10C02F101/30C02F101/34C02F101/38
CPCB01J23/885G01N21/78G01N21/31G01N21/33G01N31/10C02F1/725C02F1/722C02F2305/023C02F2101/30C02F2101/345C02F2101/38C02F2101/40
Inventor 孙萌萌鲁志伟饶含兵宋畅党阳黄舒
Owner SICHUAN AGRI UNIV
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