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Method for inhibiting FabG gene expression and inhibiting helicobacter pylori growth by using kaempferol

A Helicobacter pylori and gene expression technology, applied in the field of microbiology, can solve the problems of unfavorable human health, few reports, and the inability of people with allergies to take antibiotics, etc.

Pending Publication Date: 2021-09-10
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, taking too much antibiotics will cause many side effects, which is not conducive to human health
And some people with allergies cannot take relevant antibiotics
[0003] Therefore, it is necessary to seek non-antibiotic drugs for inhibiting gastric Helicobacter pylori, and then realize the effective treatment of gastric diseases such as gastritis, peptic ulcer, lymphoproliferative gastric lymphoma, and there are few reports in the current prior art

Method used

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  • Method for inhibiting FabG gene expression and inhibiting helicobacter pylori growth by using kaempferol
  • Method for inhibiting FabG gene expression and inhibiting helicobacter pylori growth by using kaempferol
  • Method for inhibiting FabG gene expression and inhibiting helicobacter pylori growth by using kaempferol

Examples

Experimental program
Comparison scheme
Effect test

example 3

[0042] For example, in Example 2 of the present invention, it was observed by scanning electron microscopy (SEM) that gastric Helicobacter pylori was treated with 0.1 mM kaempferol and two kinds of antibiotics at the same concentration, the external morphological changes of the bacteria, and the damage of the outer membrane of the bacteria. , so the degree of destruction of the bacteria can be visually compared. The degree of destruction of kaempferol to bacteria is between the two antibiotics, stronger than that of amoxicillin and slightly weaker than that of clarithromycin, and it is observed that the concentration is 0.1 The mM kaempferol lysed the bacterial body, the bacterial wall and bacterial membrane ruptured and curled, causing the bacterial death. Utilize transmission electron microscope (TEM) to observe gastric Helicobacter pylori in the example 3 of the present invention under the kaempferol of 0.1mM and two kinds of antibiotics of equal concentration, the ultrastru...

example 1

[0057] Example 1: Transcriptome analysis of gastric Helicobacter pylori treated with kaempferol and two antibiotics (clarithromycin, amoxicillin)

[0058] Experimental treatment: the activated gastric Helicobacter pylori was treated with 3-5% O 2 , 10% CO 2 , At 37°C, shake culture at 150rpm to the logarithmic phase, centrifuge at 5000g, 4°C for 10min, discard the supernatant, rinse the cells with 0.1M PBS, and then resuspend the cells with 0.1M PBS to OD=0.8, with kaempferol, clarithromycin and amoxicillin with a final concentration of 0.1 mM under microaerobic conditions (3-5% O 2 ,10%CO 2 ) at 37°C and 150rpm shaker for 48h, the treated bacteria samples were centrifuged and the supernatant was discarded, and the bacteria were sent to Beijing Nuohezhiyuan Bio-Information Technology Co., Ltd. at -20°C for transcriptome sequencing.

[0059] Since fatty acids and their derivatives are important components of Gram-negative cell walls and cell membranes, they are crucial to th...

example 2

[0062] Example 2: Scanning Electron Microscope (SEM) Observation of Bacteria Membrane Morphological Structure

[0063] (1) Bacteria treatment: Cultivate gastric Helicobacter pylori to the logarithmic phase, the method is the same as Example 1, and resuspend with 0.1M PBS buffer after centrifugation until the bacterial concentration is OD 600 = 0.8.

[0064] (2) Preparation of medicinal solution: Weigh kaempferol and the above two antibiotic powders into a 1.5ml centrifuge tube and dissolve with DMSO to make the final concentration 200mM. Dilute the mother solution with sterile TSB liquid medium to make the diluted working solution 50mM.

[0065]Experimental treatment: use kaempferol, two kinds of antibiotics and negative control group to treat gastric Helicobacter pylori respectively: take three milliliters of the above bacterial liquid in a 10 ml centrifuge tube, add 6 μl of the 50 mM diluted working solution above to make the final concentration 0.1 mM, a total of 4 treatm...

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Abstract

The invention provides a technology for inhibiting an FabG gene expression of by using kaempferol(Kaempferol)so as to inhibit the growth and reproduction of helicobacter pylori. Specifically, a natural product kaempferol is applied to down-regulate expression of a key gene FabG participating in synthesis of helicobacter pylori membrane lipid, the purpose of inhibiting growth and reproduction of the helicobacter pylori is achieved, and a brand new thought is provided for treating stomach diseases caused by the helicobacter pylori.

Description

technical field [0001] The invention belongs to the field of microbiology, and in particular relates to a method for inhibiting the expression of FabG gene and the growth of gastric Helicobacter pylori by using Kaempferol. Background technique [0002] Gastric Helicobacter pylori (HP) is a Gram-negative bacillus, mainly distributed in the gastric mucosa of humans and animals, and is the main cause of gastric ulcer and duodenal ulcer. At present, the clinical inhibition and elimination of gastric Helicobacter pylori is mainly through taking antibiotics such as clarithromycin and amoxicillin. However, taking too much antibiotics can cause many side effects, which is not conducive to human health. And some people with allergies cannot take relevant antibiotics. [0003] Therefore, it is necessary to seek non-antibiotic drugs for inhibiting gastric Helicobacter pylori, thereby realizing effective treatment of gastric diseases such as gastritis, peptic ulcer, lymphoproliferativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352C12N1/20A61P31/04A61P1/04A61P35/00C12R1/01
CPCA61K31/352C12N1/20A61P31/04A61P1/04A61P35/00
Inventor 李鹏民杨蕊嘉
Owner NORTHWEST A & F UNIV
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