Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of corydalis incisa amine

A technology of cordydine and carved leaves, which is applied in the field of natural medicinal chemistry, and can solve problems such as unfavorable industrialization, pungent odor, and insufficient purity of cordydine

Pending Publication Date: 2021-09-10
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Zhang Ying et al., "Analysis of the Alkaloid Components of Corydalis" [J]. Journal of Plant Resources and Environment, 2008, 17(4): 67-69 provides a preparation method of Corydalis, but the method It is obtained by extracting and separating from Corydalis genus Papaveraceae, and the separation and purification reagents used in it, such as petroleum ether, chloroform, ammonia, etc., are poisonous and have a pungent smell, which is heavy on environmental pollution. It is beneficial to industrial promotion and use, and the purity of the obtained corydalide amine is not enough to be used for the content calibration of raw materials or preparations containing corydalis amine. Therefore, it is urgent to establish a simple process and large-scale preparation of fennel Monomer alkaloid method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of corydalis incisa amine
  • Preparation method of corydalis incisa amine
  • Preparation method of corydalis incisa amine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1, the preparation of Corydalis amine of the present invention

[0036] 1. Take 1 kg of dried fennel fennel, crush it, add 70% ethanol with 10 times its mass w / w, g / g, reflux extraction 3 times, each time for 2 hours, filter and combine the filtrates, i.e. filtrate A, The filtrate is concentrated under reduced pressure at a vacuum degree of 50-250mbar and a temperature of 40-60°C to a thick paste with a relative density of 1.1 to obtain a sample of the crude extract of fennel fennel;

[0037] 2. Take the ethanol crude extract sample of anise fennel described in step 1, which is adsorbed by a D101 type macroporous resin column with a column diameter ratio of 1 / 20-1 / 5, followed by 5 times column volume water, 5 times column volume Elute with 70% ethanol by volume, discard the water-washed part, collect the eluted part with 70% alcohol, concentrate and dry under reduced pressure at a vacuum degree of 50-250mbar and a temperature of 40-60°C to obtain a sample of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of corydalis incisa amine. The preparation method comprises the following steps of: (1) taking hypecoum leptocarpum, crushing the hypecoum leptocarpum, adding ethanol for extraction, filtering and concentrating the hypecoum leptocarpum into thick paste with the relative density of 1.1-1.3; (2) taking the thick paste in the step (1), loading the thick paste on a macroporous resin column, sequentially eluting with water and ethanol, collecting an ethanol elution part, concentrating and drying to obtain a hypecoum leptocarpum refined extract; and (3) dissolving the hypecoum leptocarpum refined extract obtained in the step (2) in methanol, filtering, separating filtrate by using preparative high performance liquid chromatography, collecting a chromatographic peak fraction with the highest response value in a preparative chromatogram, concentrating and drying the fraction to obtain the corydalis incisa amine. The purity of the corydalis incisa amine prepared by the preparation method disclosed by the invention is greater than 98%.

Description

technical field [0001] The invention relates to the technical field of natural medicinal chemistry, in particular to a preparation method of corydone amine. Background technique [0002] Hypecoum leptocarpum Hook.f.et Thoms. is a plant of the genus Hypecoum leptocarpum Hook.f.et Thoms. It is one of the authentic varieties of traditional Tibetan medicine Hypecoum leptocarpum Hook.f.et Thoms. , Hepatitis, cholecystitis, joint pain, sore throat, red eyes, food poisoning and other effects. Due to the lack of chemical reference substances, the current Tibetan medicine standards promulgated by the Ministry of Health only have microscopic identification items for its quality control. [0003] According to the research on the chemical constituents of fennel, which can be consulted in the current literature, more than 20 alkaloid compounds have been reported, including hypecorine, hypecorinine, protopine, Coptis chinensis Coptisine, allocryptopine, corydam-ine, L-N-methyltetrahydro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D491/056
CPCC07D491/056
Inventor 文怀秀苑祥陶燕铎邵贇梅丽娟
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com