Optical fiber LSPR aptamer biosensor, and preparation method and application thereof
A technology of aptamer sensor and biosensor, applied in the direction of phase influence characteristic measurement, etc., can solve the problems of reducing sensor signal generation, agglomeration, deformation, etc.
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Embodiment 1
[0037] Example 1 Preparation and Characterization of GO / Au NPs Composite Material
[0038] 1) In this experiment, GO dispersed in the water phase was used as a carrier, and chloroauric acid was reduced in situ on its surface to generate AuNPs, and the GO / AuNPs composite material was obtained.
[0039] 10.0 mg of graphene oxide (GO) was ultrasonicated in 40 mL of water for 1.5 h to fully disperse GO and obtain a 0.25 mg / mL GO aqueous solution. Add 80.0 mg of polyvinylpyrrolidone (polyvinylpyrrolidone, PVP) and 0.2 mL of polydiallyl propylene dimethyl ammonium chloride (Poly dimethyl diallyl ammonium chloride PDDA) into 20 mL of 0.25 mg / mL GO solution, stir for 30 min, and the obtained The solution was centrifuged (rotating speed 10000r / min, time 10min), the supernatant was removed, washed with ethanol and deionized water, centrifuged, and repeated three times to finally obtain 5mL functionalized GO solution. Take 500uL of 1.0% chloroauric acid solution and 2.0mL GO solution, m...
Embodiment 2
[0041] Preparation and Characterization of Embodiment 2 Optical Fiber LSPR Sensor
[0042] Immerse the optical fiber in the piranha solution for 30 minutes, heat it at 70°C to remove the organic matter on the end face of the optical fiber, rinse it with ultrapure water, and dry it. 40 μL of the GO / Au NPs composite material prepared in Example 1 was drop-coated onto the end face of the optical fiber, and dried in a desiccator. The nucleic acid aptamer (5'-SH-(C 2 h 5 ) 6 -GTATAT CAAGCATCG CGT GTT TAC ACATGC GAG AGG TGAA, manufactured by Shanghai Bioengineering Co., Ltd.), configured as a solution with a concentration of 1 μM, immersed the optical fiber in the aptamer solution, and stored at room temperature for 12 hours. Then it was repeatedly washed with Tris-HCl buffer to remove unbound aptamers, and finally dried with nitrogen to complete the construction of the fiber optic LSPR sensor.
[0043] Figure 4 A is the color change before and after dispensing GO / Au NPs compo...
Embodiment 3
[0044] Example 3 Optimization of GO / Au NPs material dispensing amount
[0045] 5 μL, 10 μL, 20 μL, 40 μL, 60 μL, and 80 μL of the GO / Au NPs composite material prepared in Example 2 were used to drop-coat the optical fiber, and the reflected light of the optical fiber sensor was compared. Figure 5 A is the absorbance spectrum of the optical fiber sensor at different dispensing concentrations. It can be seen from the figure that as the dispensing concentration increases, the absorbance of the sensor end face increases continuously; Figure 5 B more intuitively shows the relationship between the amount of GO / AuNPs composite material dispensing and the LSPR peak intensity of the fiber sensor, which is determined by Figure 5 It can be seen that when the dispensing amount is higher than 40 μL, the increase of the absorbance of the end face of the optical fiber sensor tends to be gentle. In summary, the optimal drop-coating volume of the GO / Au NPs composite used in this experiment...
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