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Hemostatic material and preparation method thereof

A technology for hemostatic material and animal tissue, applied in the field of medical materials, can solve the problems of complex preparation process, increase cytotoxicity, improve effect, etc., and achieve the effects of simple preparation process, wide source and short use time

Active Publication Date: 2021-09-17
NINGBO FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The preparation process of the existing acellular matrix hemostatic materials is generally complicated, and the addition of cross-linking agents increases the cytotoxicity to a certain extent, and the effect needs to be further improved

Method used

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  • Hemostatic material and preparation method thereof
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  • Hemostatic material and preparation method thereof

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Experimental program
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preparation example Construction

[0033] The preparation of decellularized tissues and organs involves the removal of cellular and genetic material from the tissue or organ, leaving the complex ultrastructural extracellular matrix (ECM) as a natural scaffold. The technologies currently used in decellularization include physical, chemical and biological enzymatic methods, each of which has advantages and disadvantages. Two important criteria to be met during decellularization are: (i) preservation of native structure and (ii) removal of maximum cellular components. The choice of decellularizing agent depends on the nature of the tissue or organ. The present invention has no special requirements on the method of decellularization, and the existing decellularization methods can be used. Available decellularization methods include but are not limited to physical methods including freeze-thawing, direct pressure, electroporation, ultrasound and stirring; chemical methods mainly It is a variety of chemical reagents...

Embodiment 1

[0042] Example 1: Effects of Different Tissues on Hemostatic Materials

[0043] 1) Pig skin, pig liver, pig kidney, rabbit skin, and fish skin were respectively used for decellularization to prepare hemostatic materials.

[0044] 2) After the decellularization treatment, it is cleaned, freeze-dried and pulverized to obtain tissue powder;

[0045]3) Add the tissue powder into the solution, and add pepsin to digest for 8-36 hours (fish skin for 8 hours, rabbit skin for 12 hours, pig liver and kidney for 12 hours, and pig skin for 24 hours); after digestion, adjust the pH to 7.2-7.4;

[0046] 4) Afterwards, add phosphate buffer saline PBS to the digestion solution, so that the concentration of the phosphate buffer solution in the digestion solution is 0.001 M, and the processing time is 15 minutes;

[0047] 5) After being treated with PBS, it is washed, dried, and molded to prepare a hemostatic sponge material.

[0048] The appearance photo of the hemostatic material is as foll...

Embodiment 2

[0049] Example 2: Effects of Different Decellularization Methods on Hemostatic Materials

[0050] Pig skin was decellularized by stirring method, chemical method (same as in Example 1) and biological enzyme method, and then the sheet-shaped hemostatic material was prepared in the same way (same as in Example 1). A rat liver hemorrhage model was prepared, the rat liver was exposed, a hole punch was punched into a round hole with a diameter of 0.8 cm and a depth of 3 mm, and a hemostatic material was placed on it to observe the hemostatic effect.

[0051] The result is as figure 2 As shown (in the figure, A is the liver hemorrhage model, the left picture is the control group, the blood coagulates itself without adding materials, the right picture is used to stop bleeding in the liver; the left picture of B is the liver bleeding time of the control group and the hemostatic material group Determination, the hemostatic material significantly shortened the bleeding time of the liv...

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Abstract

The invention discloses a hemostatic material and a preparation method thereof. After animal tissues are subjected to decellularization treatment, freeze-drying and crushing are performed, pepsin is further digested, then low-concentration PBS is used for carrying out treatment, and after-treatment is performed to obtain the hemostatic material. The hemostatic materials provided by certain embodiments of the invention have good biocompatibility and hemostatic performance, the preparation technology is simpler, and the cost is low. The preparation methods of certain embodiments do not need crosslinking treatment, is simple and convenient in operation, and has a high success rate and short consumed time. According to the preparation methods of certain embodiments of the invention, the coagulation time of the hemostatic material can be conveniently regulated and controlled by controlling of the concentration of PBS.

Description

technical field [0001] The invention relates to medical materials, in particular to a hemostatic material and a preparation method thereof. Background technique [0002] Hemostasis is a critical step in both trauma and surgery. Timely and effective hemostasis can accelerate wound healing and even ensure life safety. For different degrees of bleeding, the hemostatic techniques adopted will be different. Severe bleeding may require clipping or direct blockage of blood vessels, while for more common bleeding caused by mild trauma, surgical cuts, postoperative complications, etc. , the use of an external hemostatic material would be more appropriate. [0003] Various hemostatic materials have been developed in the prior art and are constantly being improved. At present, hemostatic materials are mainly divided into inorganic hemostatic materials and natural hemostatic materials. The inorganic components used to prepare hemostatic materials are mainly zeolite, clay, kaolin, meso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/00
CPCA61L24/0005A61L24/001A61L2400/04
Inventor 王芳芳梅劲张蕊高妞妞
Owner NINGBO FIRST HOSPITAL