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Composite microbial agent applicable to out-of-situ fermentation bed as well as preparation method and application of composite microbial agent

A technology of compound bacterial agent and fermentation bed, which is applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve the problems of high cost, limited manure treatment effect, and few types of fermentation bacteria, etc. Low, good fermentation effect, scientifically designed effect

Inactive Publication Date: 2021-09-24
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are not many types of fermentation bacteria in the ectopic fermentation bed market, and most of them are probiotics fed to animals in situ fermentation beds, which have limited treatment effect on manure and high cost

Method used

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  • Composite microbial agent applicable to out-of-situ fermentation bed as well as preparation method and application of composite microbial agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment discloses the preparation method of bacterial agent of the present invention:

[0032] (1) Preparation of Aspergillus fumigatus fungal agent: inoculate Aspergillus fumigatus on PDA medium, cultivate for 3 days at 28° C., then inoculate 5% inoculum amount by volume percentage in nutrient broth culture medium for expanded cultivation, to obtain final product;

[0033] (2) Preparation of yeast inoculum: inoculate yeast in PDA medium, cultivate at 28°C for 2 days, then inoculate in nutrient broth culture medium with an inoculation amount of 5% by volume to expand the culture, to obtain final product;

[0034] (3) Preparation of Bacillus Potsdam bacteria agent: inoculate Bacillus Potsdam in LB culture medium, cultivate at 37° C. for 2 days, then inoculate 5% inoculum by volume in nutrient broth culture solution for expanded cultivation, to obtain final product;

[0035] (4) Preparation of Rhodopseudomonas faecalis bacteria agent: inoculate Rhodopseudomonas fa...

Embodiment 2

[0039] This embodiment discloses the composition of the composite bacterial agent of the present invention, specifically as shown in the following table:

[0040] Table 1

[0041] Group 1 2 teams 3 groups 4 groups Aspergillus fumigatus (part) 20 15 20 25 Yeast Inoculant (part) 20 20 15 10 Bacillus Potsdam bacteria agent (part) 15 20 20 25 Rhodopseudomonas faecalis agent (part) 15 15 15 10 Lactobacillus plantarum agent (part) 15 15 15 10 Streptomyces lividans (parts) 15 15 15 20

Embodiment 3

[0043] This embodiment discloses the preparation method of the composite bacterial agent of the present invention: prepare Aspergillus fumigatus bacterial agent, saccharomyces bacterial agent, Potsdamian bacterial agent, Rhodopseudomonas faecalis bacterial agent, Lactobacillus plantarum bacterial agent and Strep.

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Abstract

The invention discloses a composite microbial agent applicable to an out-of-situ fermentation bed as well as a preparation method and application of the composite microbial agent and belongs to the technical field of biological fermentation. The composite microbial agent applicable to the out-of-situ fermentation bed, provided by the invention, comprises the following components in parts by volume: 15-25 parts of an Aspergillus fumigatus microbial agent, 10-20 parts of a Brevibacillus borsteri microbial agent, 15-25 parts of a Rhodopseudomonas faecalis microbial agent, 10-20 parts of a Lactobacillus plantarum microbial agent, 10-20 parts of a Saccharomyces microbial agent and 10-20 parts of a Streptomyces lividus microbial agent. The preparation method disclosed by the invention comprises the following steps: preparing all the microbial agents according to the parts by volume, and carrying out uniform mixing, thereby obtaining the composite microbial agent. The composite microbial agent provided by the invention is applied to the out-of-situ fermentation bed for treatment of livestock and poultry manure. The method is scientific in design and ingenious in conception, and non-probiotic strains are applied to out-of-situ-bed fermentation for the first time. The composite microbial agent disclosed by the invention has a good fermentation effect, can be used for effectively treating the manure, and is lower in cost compared with a sold microbial agent.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a composite bacterial agent suitable for an ectopic fermentation bed, a preparation method and an application. Background technique [0002] With the development of my country's animal husbandry, the problem of livestock and poultry breeding waste pollution is becoming increasingly serious. Heterotopic fermentation bed is a green technology for manure treatment, which can make livestock and poultry waste pollution-free, zero-emission, and odor-free ecological pig raising mode. The ectopic fermentation bed technology is to collect the cultured manure, add suitable strains, spray the manure evenly on the bedding in the fermentation tank, and use the turner to turn the rake to fully mix the manure and bedding Fully ferment under the action of microorganisms to degrade or decompose organic substances such as crude protein, crude fat, residual starch, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/16C12N1/20C02F11/02C02F103/20C12R1/68C12R1/08C12R1/38C12R1/25C12R1/465C12R1/645
CPCC12N1/14C12N1/16C12N1/20C02F11/02C02F2103/20
Inventor 罗玲何钟麒袁龙杜祥祥何燕王莉淋张延宗刘燕
Owner SICHUAN AGRI UNIV