Ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material as well as preparation method and application thereof

A technology of hyaluronic acid and nanomaterials, applied in the direction of nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of short drug release time and uncontrollability, achieve excellent antibacterial effect, various forms, and prevent wound infection.

Active Publication Date: 2021-09-28
温州医科大学附属口腔医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of short and uncontrollable sustained release of drugs in the prior art, and to provide an ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial. The inclusion compound formed by dextrin inclusion active drug is loaded on the mesoporous silica nanoparticles, so that the active drug can be double sustained release, realizing the ultra-long-term sustained release of the active drug, combined with the coating on the mesoporous silica The hyaluronic acid on the surface of nanoparticles realizes the pH-responsive release of drugs and realizes the controllable long-term release of active drugs

Method used

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  • Ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material as well as preparation method and application thereof
  • Ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material as well as preparation method and application thereof
  • Ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material as well as preparation method and application thereof

Examples

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Effect test

preparation example Construction

[0049] According to the examples disclosed in the present invention, the present invention exemplarily proposes a method for preparing an ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial, which specifically includes the following steps:

[0050] First fully mix the acetone solution of the active drug with the cyclodextrin aqueous solution, filter and vacuum dry to obtain the clathrate;

[0051] Dissolving the clathrate in a buffer solution, mixing and reacting with the mesoporous silica nanoparticles dissolved in the buffer solution, to obtain a solution of mesoporous silica nanoparticles loaded with clathrates;

[0052] Then mix the hyaluronic acid dissolved in the buffer solution with the mesoporous silica nanoparticle solution loaded with clathrate, stir and centrifuge to obtain the first-generation mesoporous-hyaluronic acid hybrid nanoparticle;

[0053] Dissolving the first-generation mesoporous-hyaluro...

Embodiment 1

[0089] Synthesis of CHX-CD

[0090] Add 2.5mg / 10mL chlorhexidine acetone solution dropwise into 10mg / 10mL β-CD aqueous solution, wherein the volume ratio of the two is 1:1, and then fully mix and stir for 24 hours, after suction filtration and separation, vacuum drying to obtain chlorhexidine -Cyclodextrin inclusion complex (CHX-CD) is preserved for future use.

[0091]Characterization of CHX-CD

[0092] Fourier infrared:

[0093] Take an appropriate amount of sample (3.0mg), add potassium bromide powder (200mg), put it into an agate mortar under the irradiation of an infrared lamp, grind the two fully and evenly, and then transfer the ground fine powder to the matching tablet press In, a thin sheet with a thickness of about 0.5mm was made. Taking the blank disc as a control, the prepared thin slices were put into a Fourier transform infrared spectrometer for determination.

[0094] Proton NMR spectrum:

[0095] Dissolve the clathrate in D2O, prepare a solution with an ap...

Embodiment 2

[0101] Preparation of HA-CHX-CD-MSNs

[0102] Dissolve 1.0g of CTAB in 480mL of deionized water, then add 3.5mL of 2mol / L NaOH, mix thoroughly, add 7.0mL of mesitylene, and stir vigorously in a water bath at 80°C for 2h.

[0103] Subsequently, 5.0 mL of TEOS was added dropwise, and the above temperature was kept at 80° C. and vigorously stirred for 2 h to form a white precipitate.

[0104] The product after the reaction was vacuum filtered, washed with a large amount of methanol solution, and dried overnight in vacuum, the initial product of MSN.

[0105] Take 1.0g of the dried material and disperse it in 100mL of methanol solution, then add 0.75mL of concentrated hydrochloric acid, stir in a 50°C water bath for 6h, and remove the template agent, mainly CTAB.

[0106] After the reaction was completed, they were filtered and washed by suction, dried in vacuum overnight, and used for later use to obtain MSNs.

[0107] Take 10 mg of the clathrate CHX-CD obtained in Example 1 an...

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Abstract

The invention provides an ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material. The nano-material comprises a drug carrier, an active drug and hyaluronic acid, wherein the drug carrier is mesoporous silica nanoparticles; the active drug is included in cyclodextrin to form an inclusion compound; the inclusion compound is loaded on the mesoporous silica nanoparticles; and the hyaluronic acid wraps the surfaces of the mesoporous silica nanoparticles loaded with the inclusion compound, so that the mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial is formed. The invention also provides a preparation method and application of the ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nano-material. According to the nano-material disclosed by the invention, the active drug can be subjected to dual slow release, the ultra-long-acting slow release of the active drug is realized, the pH response release of the drug can be realized, and the long-acting release of the active drug is controllable.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to an ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial and its preparation method and application. Background technique [0002] Chronic periodontitis is a chronic oral infectious disease caused by periodontal pathogenic bacteria infection. The current clinical treatment is mainly based on mechanical removal of periodontal pathogenic bacteria. However, due to the existence of areas that cannot be reached by instruments and the easy recolonization of periodontal pathogenic bacteria, local antimicrobial therapy is still required. [0003] Chlorhexidine (CHX), as a biologically active antibacterial agent, has the characteristics of broad-spectrum antibacterial, bactericidal and low toxicity, and is widely used in periodontal antibacterial treatment. However, current periodontal topical antibacterial drugs generally...

Claims

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Application Information

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IPC IPC(8): A61K31/155A61K47/69A61K47/04A61K47/36A61P1/02A61P29/00A61P31/04A61P17/02B82Y5/00B82Y40/00
CPCA61K31/155A61K47/6951A61K47/02A61K47/36A61P1/02A61P29/00A61P31/04A61P17/02B82Y5/00B82Y40/00
Inventor 邓辉林坚何智琪王奕
Owner 温州医科大学附属口腔医院
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