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Ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial and its preparation method and use

A technology of hyaluronic acid and nanomaterials, applied in the directions of antibacterial drugs, nanotechnology, nanotechnology, etc., can solve the problems of uncontrollability and short duration of drug sustained release, and achieve various forms, excellent antibacterial effect, and cost-saving effect.

Active Publication Date: 2022-03-18
温州医科大学附属口腔医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problem of short and uncontrollable sustained release of drugs in the prior art, and to provide an ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial. The inclusion compound formed by dextrin inclusion active drug is loaded on the mesoporous silica nanoparticles, so that the active drug can be double sustained release, realizing the ultra-long-term sustained release of the active drug, combined with the coating on the mesoporous silica The hyaluronic acid on the surface of nanoparticles realizes the pH-responsive release of drugs and realizes the controllable long-term release of active drugs

Method used

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  • Ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial and its preparation method and use
  • Ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial and its preparation method and use
  • Ultra-long-acting controllable sustained-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial and its preparation method and use

Examples

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Effect test

preparation example Construction

[0049] According to an example disclosed in the present invention, the present invention is exemplarily proposed a method for preparing a super long-acting controlled reseudentable-aperture-hyaluronic acid to target antibacterial nanomaterials, and specifically includes the following steps:

[0050] First, the acetone solution of the active drug is thoroughly mixed with a cyclo paste, and the vacuum is filtered.

[0051] The incubation is dissolved in the buffer solution, mixed with the mesoporous silica nanoparticles dissolved in the buffer solution to obtain a mesoporous silica nanoparticle solution of the loaded clam;

[0052] The hyaluronic acid dissolved in the buffer solution is mixed with the mesoporous silica nanoparticle solution of the loaded comprise, stirring centrifugation, resulting in a generation of mesh-hycruronic acid hypocya;

[0053] A generation of mesopores-hyaluronic acid hyacinacid is dissolved in a buffer solution, mixed with a hyaluronic acid buffer solut...

Embodiment 1

[0089] CHX-CD synthesis

[0090] 2.5 mg / 10 ml of chlorhexehydrophone solution was added to a 10 mg / 10 ml of β-CD aqueous solution, wherein the volume ratio of both of them was 1: 1, and after sufficiently mixing for 24 h, then the filtration was separated, dried in vacuo to obtain chlorhexidane - Cyclodextrial adherate (CHX-CD) preservation spare.

[0091]Characterization of CHX-CD

[0092] Fourier transform infrared:

[0093] Proper amount of sample (3.0mg), was added potassium bromide powder (200 mg of), placed in an agate mortar under infrared lamp irradiation, polishing uniformity both sufficiently, after which the milled powder was transferred to a supporting good tableting machine , a sheet having a thickness of about 0.5mm. Disc is a blank control, the prepared sheet was measured into the FTIR.

[0094] Nuclear magnetic resonance spectrum:

[0095] The inclusion complex was dissolved in D2O, configured into the proper concentration NMR tube was then placed in the instru...

Embodiment 2

[0101] Preparation of HA-CHX-CD-MSNs of

[0102] The 1.0g CTAB was dissolved in 480mL of deionized water, was added 3.5mL 2mol / L NaOH, mix well, 7.0mL mesitylene was added, with vigorous stirring in a water bath at 80 ℃ 2h.

[0103] Followed by dropwise addition of 5.0mL TEOS, maintained at 80 ℃ above vigorously stirred 2h, a white precipitate formed.

[0104] The completed reaction product by vacuum filtration, washed with large amount of methanol and the solution was dried in vacuo overnight, MSN primary product.

[0105] The dried material was dispersed in 1.0g taken in 100mL methanol, concentrated hydrochloric acid was added 0.75mL, 50 ℃ water bath was stirred 6h, template removal, mainly CTAB.

[0106] After completion of the reaction, the filtration was washed and dried in vacuo overnight, standby, obtained MSNs.

[0107] Take 10mg clathrate obtained in Example 1 was dissolved in 10 mL CHX-CD PBS buffer solution to obtain a PBS solution 0.05mmoL / mL as a first solution, 2...

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Abstract

The present invention provides an ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial, the nanomaterial includes a drug carrier, an active drug and hyaluronic acid, wherein the drug carrier is mesoporous dioxide Silicon nanoparticles, the active drug is included in the cyclodextrin to form an inclusion compound, the inclusion compound is loaded on the mesoporous silica nanoparticles, and the hyaluronic acid is wrapped in the inclusion compound-loaded medium Porous silica nanoparticles surface to form mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterials. The present invention also provides a preparation method and application of an ultra-long-acting controllable slow-release mesoporous-hyaluronic acid hybrid targeted antibacterial nanomaterial. The nanometer material of the present invention can achieve double sustained release of active drugs, realize ultra-long-term sustained release of active drugs, and realize pH-responsive release of drugs, and controllable long-term release of active drugs.

Description

Technical field [0001] The present invention relates to the field of biomaterial technology, and in particular, a super long-acting controlled reseudently-free-hyperotoricate targeting antibacterial nanomaterial and a preparation method thereof. Background technique [0002] Chronic periodontitis is an oral chronic infectious disease caused by pathoplasm infection. At present, clinical treatment is mainly based on mechanical removal of periodontal pathogenic bacteria. However, due to the fact that there is an area where the device cannot reach, it is still necessary to supplement local antibacterial therapy. [0003] Chlorhexidine, CHX) As a biologically active antibacterial agent, it has the characteristics of broad-spectrum antibacterial, bactericidal, and low toxicity, and is widely used in periodontal antibacterial therapy. However, there is a shortest point of local antibacterial drugs in the periodontal cycle, and the shortcomings of multiple administration and non-inflamma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/155A61K47/69A61K47/04A61K47/36A61P1/02A61P29/00A61P31/04A61P17/02B82Y5/00B82Y40/00
CPCA61K31/155A61K47/6951A61K47/02A61K47/36A61P1/02A61P29/00A61P31/04A61P17/02B82Y5/00B82Y40/00
Inventor 邓辉林坚何智琪王奕
Owner 温州医科大学附属口腔医院
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