2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor

A technology of pyridines and aryls, applied in the field of medicinal chemistry, to achieve good selectivity, facilitate popularization and application, and good in vitro anti-tumor activity

Active Publication Date: 2021-09-28
XINXIANG MEDICAL UNIV
View PDF13 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to discover new LSD1/HDAC dual-target inhibitors, explore the synthesis of a class of 2-aryl-4-(1H-pyrazol-3-yl)pyridine compounds, verify their LSD1, HDAC dual-target inhibitory activity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor
  • 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor
  • 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Synthesis of 2-bromo-4-(1H pyrazol-3-yl)pyridine (3)

[0024]

[0025] Add compound 2-bromo-4-iodopyridine (1212.1mg, 6.0mmol), toluene (20mL), ethanol (25mL), H 2 O (7.5mL), K 2 CO 3 (1.68g, 11.1mmol), Pd(PPh 3 ) 4(694.0mg, 0.6mmol) and pyrazole-3-boronic acid (1451.9mg, 7.2mmol), stirred and reacted at 92°C for 4 hours under nitrogen protection. After the reaction, the reaction system was cooled to room temperature, and then added to the reaction system Extract with water and ethyl acetate, combine the ethyl acetate layers, wash with water and saturated NaCl aqueous solution respectively, dry over anhydrous sodium sulfate, filter, concentrate the filtrate under reduced pressure, and separate and purify the concentrate by silica gel column chromatography (petroleum ether: acetone = 5:1) to obtain compound 3a (850.4mg), white solid, yield: 65.5%, Mp: 140-141℃. 1 H NMR (400MHz, DMSO-d 6 )δ13.36(s,1H),8.39(d,1H,J=5.2Hz),8.03(d,1H,J=1.6Hz),7.88(s,1H),7.8...

Embodiment 2

[0026] Example 2 Synthesis of methyl 4-((3-(2-bromo-pyridin-4-yl)-1H-pyrazol-1-yl)methyl)benzoate (4)

[0027] Add compound 3 (1244.4mg, 6.0mmol), methyl 4-bromomethylbenzoate (1451.9mg, 7.2mmol) and cesium carbonate (2932.2mg, 9.0mmol) in a 50ml two-necked flask, add DMF (8mL), Stir the reaction at room temperature under nitrogen protection for 5 h. Then add water and ethyl acetate to the reaction system for extraction, combine the ethyl acetate layers, wash with water and saturated NaCl aqueous solution, dry over anhydrous sodium sulfate, filter, and concentrate the filtrate under reduced pressure. Separation and purification by silica gel column chromatography (petroleum ether: acetone = 2:1) gave compound 4 (1755.4 mg), a white solid, yield: 78.6%, Mp: 127-128°C. 1 H NMR (400MHz, CDCl 3 )δ8.36 (d, 1H, J = 5.2Hz), 8.03 (d, 2H, J = 8.0Hz), 7.90 (d, 1H, J = 1.2Hz), 7.64 (dd, 1H, J 1 =1.6Hz,J 1 =5.2Hz),7.47(d,1H,J=2.4Hz),7.29(d,2H,J=8.4Hz),6.69(d,1H,J=2.4Hz),5.43(s,2H),3....

Embodiment 3

[0028] Example 3 Synthesis of 4-((3-(2-(4-methylphenyl)pyridin-4-yl)-1H-pyrazol-1-yl)methyl)benzoate (5a)

[0029]

[0030] In a 50 ml two-neck round bottom flask, add compound 4 (521.1 mg, 1.4 mmol), toluene (5 mL), ethanol (5 mL), H 2 O (1.3mL), K 2 CO 3 (359.3mg, 2.6mmol), Pd(PPh 3 ) 4 (162.0mg, 0.14mmol) and 4-methylphenylboronic acid (231.1mg, 1.7mmol), under nitrogen protection, stirred at 92°C for 4 hours. After the reaction, the reaction system was cooled to room temperature, extracted with water and ethyl acetate , the ethyl acetate layers were combined, washed with water and a saturated NaCl aqueous solution, dried over anhydrous sodium sulfate, and filtered with suction, the filtrate was concentrated under reduced pressure, and the concentrate was purified by silica gel column chromatography (petroleum ether: ethyl acetate = 2:1), to obtain compound 5a (483.7mg), yield: 90.1%, white solid, Mp: 92-93°C. 1 H NMR (400MHz, CDCl 3 )δ8.68(dd,1H,J 1 =0.8Hz,J 2 =...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor and a preparation method thereof, and application of the 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1/HDAC double-target inhibitor in preparation of antitumor drugs, belonging to the technical field of medicinal chemistry. The inhibitor has a general formula as described in the specification. In the general formula, R1 is preferably selected from CH3, OCH3 and H; and R2 is preferably F and H. The inhibitor disclosed by the invention has relatively strong inhibitory activity on both LSD1 and HDAC, and the IC50 value of the inhibitor on HDAC1 is less than 5 nM, and the inhibitor is remarkably superior to a positive drug SAHA. The LSD1/HDAC double-target inhibitor has good in-vitro anti-tumor activity on human acute myelogenous leukemia THP-1 cell strains, is superior to a positive drug SAHA, provides a basis for research and development of LSD1/HDAC double-target inhibitor drugs, and can be used as a candidate or a lead compound for further development for development of anti-tumor treatment drugs.

Description

technical field [0001] The invention specifically relates to a class of 2-aryl-4-(1H-pyrazol-3-yl)pyridine LSD1 / HDAC dual-target inhibitors, its preparation method and its application in the preparation of antitumor drugs, belonging to medicinal chemistry technology field. Background technique [0002] The dysregulation of epigenetic modification is closely related to the occurrence, development and poor prognosis of tumors. The development of anticancer drugs targeting epigenetic regulatory proteins has aroused great interest and has made great progress. [0003] Histone lysine-specific demethylase 1 (LSD1), also known as KDM1A, CPRF, BHC110 and AOF2, was discovered by Shi Yang's team at Harvard University in 2004. LSD1 is a flavin adenine dinucleotide-dependent amino oxidase whose main function is to specifically remove monomethylation and dimethylation on histone H3K4. LSD1 represses the transcription of genes by removing the methylation marks of H3K4me1 / 2, thereby regu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D401/04A61P35/02
CPCC07D401/04A61P35/02
Inventor 段迎超关圆圆张少杰于童靳林峰
Owner XINXIANG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap