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Preparation method of modified biological material and obtained modified biological material

A biomaterial modification technology, which is applied in tissue regeneration, pharmaceutical formulation, drug delivery, etc., can solve the problems that the lifespan of biomaterials cannot be improved, the application of glutaraldehyde is troublesome, and biomaterials are easy to calcify, so as to reduce the residue of aldehyde groups , increase cross-linking sites, and improve anti-calcification performance

Active Publication Date: 2021-10-15
KOKA NANTONG LIFESCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biomaterials treated with glutaraldehyde are easy to hydrolyze and glutaraldehyde is toxic, which makes the biomaterials easy to calcify, so the lifespan of the biomaterials cannot be improved, and glutaraldehyde itself has certain toxicity. , which have caused great troubles to the application of glutaraldehyde and biomaterials

Method used

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  • Preparation method of modified biological material and obtained modified biological material
  • Preparation method of modified biological material and obtained modified biological material
  • Preparation method of modified biological material and obtained modified biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Firstly, the fresh bovine pericardium was rinsed N=2 times with normal saline with a mass fraction of 0.9%, and the bovine pericardium was pretreated. Then, the pressure is P=100Pa, the discharge power is W=200W, and the pretreated bovine pericardium is treated with low-temperature plasma under ammonia atmosphere conditions. 1 = 60 min. Finally, under the condition of T=normal temperature, the mass fraction is C=0.3%, soaking t in the glutaraldehyde solution of pH value=6.5 2 = 12h, the cross-linking reaction between the glutaraldehyde solution and the bovine pericardium treated with low-temperature plasma occurred.

Embodiment 2

[0030] Select fresh bovine pericardium, rinse the fresh bovine pericardium with physiological saline for 3 times to remove impurities such as fat fibers on the surface, put the bovine pericardium into a low-temperature plasma device, set the pressure at 80Pa in an oxygen atmosphere, and discharge The power is 200W, and the processing time is 10min.

[0031] The bovine pericardium modified by low-temperature plasma was soaked in a glutaraldehyde solution with a mass concentration of 0.1%, the soaking temperature was 30°C, the crosslinking time was 3h, and the pH of the glutaraldehyde solution was 6.8.

Embodiment 3

[0033] Choose fresh bovine pericardium and rinse the fresh bovine pericardium with normal saline to remove impurities such as fat fibers on the surface, then put the bovine pericardium into the low-temperature plasma equipment, set the pressure at 80Pa, discharge power at 300W, and process time for 10 minutes .

[0034] The bovine pericardium modified by low-temperature plasma was soaked in 0.2% glutaraldehyde solution, the soaking temperature was 45°C, the crosslinking time was 72h, and the pH of the glutaraldehyde solution was 8.5.

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Abstract

The invention discloses a preparation method of a modified biological material and the obtained modified biological material. The method comprises a surface modification step and a crosslinking treatment step. According to the method, the surface modification step is additionally arranged, and low-temperature plasma is used for introducing active groups into a biological material, so that the activation sites of collagen molecules on the surface of the biological material are increased, the activation energy of the biological material is reduced, the binding capacity with glutaraldehyde is greatly improved, and the conversion rate of glutaraldehyde in the crosslinking process is greatly increased. Therefore, by using the method provided by the invention, in order to achieve the same cross-linking effect, only a smaller amount of glutaraldehyde with a lower concentration needs to be added, the content of residual glutaraldehyde in the modified biological material is also obviously reduced, the toxicity of glutaraldehyde to the biological material is reduced, and the anti-calcification performance of the modified biological material is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, and further relates to a preparation method of a modified biological material and the obtained modified biological material. Background technique [0002] Cardiovascular disease is the number one health killer worldwide. There are nearly 300 million people with cardiovascular disease in my country. According to incomplete statistics, about 3 million people die from cardiovascular diseases every year, accounting for 45% of the total number of deaths from all diseases. Clinically, common cardiovascular diseases mainly include coronary heart disease and heart valve disease. Heart valve disease is a common heart disease in my country, among which, valve damage caused by rheumatic fever is the most common. With the aging of the population, senile valvular disease, coronary heart disease, and heart valvular disease caused by myocardial infarction are becoming more and more common. [0...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3687A61L27/3691A61L27/3625A61L27/3629A61L27/3604A61L27/50A61L2430/20A61L2400/18
Inventor 吴明明周茜陈大凯
Owner KOKA NANTONG LIFESCIENCES CO LTD
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