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A method based on complexation to detect cu + Fluorescent probes and their applications

A fluorescent probe and complexation technology, which is applied in the field of cuprous ion fluorescence analysis and detection, can solve problems such as dysfunction of the human body system, and achieve stable chemical properties, high quantum fluorescence yield, and beneficial effects on accuracy

Active Publication Date: 2022-03-29
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, excess cuprous can lead to dysfunction of the body system

Method used

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  • A method based on complexation to detect cu <sup>+</sup> Fluorescent probes and their applications
  • A method based on complexation to detect cu <sup>+</sup> Fluorescent probes and their applications
  • A method based on complexation to detect cu <sup>+</sup> Fluorescent probes and their applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Probe preparation:

[0028] 50 μL of N-(4-methylbenzyl)-1-(pyridin-2-yl)methanamine (MDPA) and 30 μL of 25 wt% ammonia water (NH 3 ·H 2 O) Add to 2mL DCM / ethanol=1:1 mixed solvent and cool in ice bath for 30 minutes. Then add 35 μL CS dropwise 2 , after dropping, stirred at room temperature for 10 hours. A yellow solution was produced. The solution was chromatographed on a silica gel column with PET / EAC=4:1. Then vacuum dried to obtain pure product. The yield was 61.2%. Purified probe molecules were tested by high-resolution mass spectrometry and nuclear magnetic resonance: ESI-MS (m / z): 255.09 (M+H) + ;277.07(M+Na) + ; 1H NMR (CDCl3, 400MHz) δ: 8.34(d, J=7.4Hz, 9H), 7.28(m, 32H), 7.20(d, J=7.8Hz, 30H), 7.08(s, 9H), 6.85( s, 8H), 6.73 (m, 8H), 6.54 (t, J=6.8Hz, 9H), 5.49 (s, 20H). with Cu + High-resolution mass spectrometry test of the probe molecule after the reaction: ESI-MS (m / z): 339.23 (M+Na) + .

Embodiment 2

[0030] Cuprous ion solution configuration: by successively adding FeCl with the same molar mass to the probe2 and CuSO 4 solution is obtained.

[0031] With Cu + As the concentration increased, the fluorescence intensity decreased gradually, and showed a good linear relationship, with a correlation coefficient of 0.992 in the range of 0-2.8 μM. It can be used to quantify lower concentrations of Cu + . The limit of detection was 15 nM. Such as Figure 4 .

Embodiment 3

[0033] Probe selectivity and interference verification:

[0034] in Ni 2+ , Fe 3+ , Fe 2+ , Mg 2+ 、Na + 、K + , Mn 2+ 、Cd 2+ , Ca 2+ 、Ba 2+ , NH 4 + 、Cu 2+ 、Ag + , Pb 2+ To detect probe selectivity in the presence of metal ions. Adding twice the amount of other ions mixed with the probe, studied the effect of AHP on Cu + The anti-interference effect, the result is as follows Figure 5 . Even if an excessive amount of interfering metal ions is added, the fluorescence of the probe is not significantly affected. Compared with other metal ions, the probe is more sensitive to Cu + Has high selectivity.

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Abstract

A method for detecting Cu based on complexation + fluorescent probes and their applications. The invention provides a fluorescent probe AHP for the visual detection of cuprous ions, especially for rapid visual and quantitative detection of cuprous in a specific environment. This probe enables rapid and highly selective detection of Cu in an oxygen-free environment + . Sulfur and Cu in the probe + A metal complexation reaction occurs, resulting in a photoinduced electron transfer (PET) effect. The fluorescence of the probe is greatly suppressed, and the visual detection of cuprous ions is realized. And the probe itself has high quantum fluorescence yield and stable chemical properties. Therefore, a stable fluorescence intensity can be maintained in the air, which is conducive to the development of detection activities and the accuracy of experimental results. This probe has great potential in the detection and application of human cells, paving the way for future analysis and detection.

Description

technical field [0001] The invention relates to the technical field of fluorescence analysis and detection of cuprous ions, in particular to a linear detection of cuprous ions in a specific environment. Background technique [0002] Copper is an essential redox active nutrient for life. Proteins contain the necessary efficient catalytic and structural cofactors for oxygen transport, respiratory metabolism and cell growth in the human body. In addition to the copper ions contained in proteins, emerging data suggest the presence of labile cuprous ions. Due to their chemical instability, their binding to low molecular weight ligands is relatively weak. However, cuprous ions play a vital role in human activities. It promotes a growing number of dynamic transition metal signaling pathways, including neural communication, odor and lipolysis. However, an imbalance of copper ratio in the human body can lead to various diseases such as cancer, cardiovascular disease, neurodegener...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D471/04C09K11/06G01N21/64
CPCC07D471/04C09K11/06G01N21/6428C09K2211/1044C09K2211/1007G01N2021/6432
Inventor 王素华殷冉皓余龙马祥云苏鹏辰
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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