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Inorganic phosphorus detection reagent and detection chip

A detection reagent and detection chip technology, applied in measurement devices, color/spectral property measurement, material analysis by observing the impact on chemical indicators, etc., can solve problems such as poor anti-interference ability, and achieve strong anti-interference ability. Effect

Pending Publication Date: 2021-10-19
GENRUI BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem of poor anti-interference ability of inorganic phosphorus detection reagents, an embodiment of the present invention provides an inorganic phosphorus detection reagent and a detection chip, which can pass through maltose, oxidized coenzyme, β-phosphate glucose mutase, maltose phosphorylase and glucose-6-phosphate dehydrogenase to realize the detection of inorganic phosphorus, ascorbic acid, bilirubin, hemoglobin, triglyceride, uric acid and creatinine all have strong anti-interference ability

Method used

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  • Inorganic phosphorus detection reagent and detection chip
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  • Inorganic phosphorus detection reagent and detection chip

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preparation example Construction

[0056] The embodiment of the present invention also provides a preparation method of an inorganic phosphorus detection reagent, the method comprising the following steps:

[0057] S11. Acquiring reagents, the first reagent and the second reagent;

[0058] In some embodiments, the configuration process of the first reagent is as follows: add an appropriate amount of distilled water into the container, weigh the buffer components, add maltose and oxidized coenzyme in turn after the buffer solution is completely dissolved, and adjust the pH to 6.5-7.5, Finally, the excipient is added, and then the volume is constant to obtain the first reagent. In some embodiments, the configuration process of the second reagent is as follows: add an appropriate amount of distilled water into the container, weigh the buffer components, and add β-glucomutase, maltose phosphorylase, and Glucose-6-phosphate dehydrogenase, adjust the pH to 6.5-7.5, finally add excipients, and then adjust the volume ...

Embodiment 1

[0066] The raw material components of the inorganic phosphorus detection reagent in the inorganic phosphorus detection chip provided in this embodiment include:

[0067] The components of the first reagent are as follows:

[0068] PIPES buffer 20mmol / L;

[0069] EDTA 1g / L;

[0070] Glycerin 10g / L;

[0071] Maltose 50g / L;

[0072] Oxidized coenzyme I 20L;

[0073] Trehalose 100g / L;

[0074] The pH value of the first reagent was 6.7.

[0075] The components of the second reagent are as follows:

[0076] PIPES buffer 100mmol / L;

[0077] EDTA 10g / L;

[0078] β-glucomutase phosphate 10KU / L;

[0079] Maltose phosphorylase 10KU / L;

[0080] Glucose-6-phosphate dehydrogenase 20KU / L;

[0081] Trehalose 150g / L;

[0082] Dextran 10,000 50g / L;

[0083] The pH of the second reagent was 6.7.

Embodiment 2

[0085] The difference between this embodiment and embodiment 1 is as follows:

[0086] The components of the first reagent are as follows:

[0087] Bis-Tris buffer 100mmol / L;

[0088] Bovine serum albumin 10g / L;

[0089] Maltose 25g / L;

[0090] Oxidized coenzyme II 50g / L;

[0091] Mannitol 120g / L;

[0092] The pH of the first reagent was 7.0.

[0093] The components of the second reagent are as follows:

[0094] Bis-Tris buffer 100mmol / L;

[0095] Bovine serum albumin 1g / L;

[0096] β-glucomutase phosphate 20KU / L;

[0097] Maltose phosphorylase 20KU / L;

[0098] Glucose-6-phosphate dehydrogenase 40KU / L;

[0099] Dextran 40,000 50g / L;

[0100] Mannitol 80g / L;

[0101] The pH of the second reagent was 7.0.

[0102] The performance of the inorganic phosphorus detection chip obtained in Example 1 of the present invention will be described below in conjunction with the table. It should be noted that, at 37° C., the change value of the absorbance of the assay chip injec...

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Abstract

The invention relates to the technical field of inorganic phosphorus detection, and in particular relates to an inorganic phosphorus detection reagent and a detection chip. The inorganic phosphorus detection reagent and the detection chip provided by the embodiment of the invention comprise maltose, oxidized coenzyme, beta-phosphoglucose mutase, maltose phosphorylase and glucose-6-phosphate dehydrogenase. Inorganic phosphorus in a detection sample reacts with maltose to generate glucose 1-phosphate. The glucose 1-phosphate forms the glucose 6-phosphate under the action of the phosphoglucose mutase and / or the coenzyme of the phosphoglucose mutase. The glucose 6-phosphate is oxidized into the 6-phosphogluconic acid under the action of the glucose-6-phosphate dehydrogenase, meanwhile, the oxidized coenzyme is reduced into the reduced coenzyme, and the content of the inorganic phosphorus in the detection sample can be calculated according to the change of the absorbance of a reaction system at 340 nm. A test result shows that the inorganic phosphorus detection reagent and the detection chip provided by the embodiment of the invention have relatively strong anti-interference capability on ascorbic acid, bilirubin, hemoglobin, triglyceride, uric acid and creatinine.

Description

technical field [0001] The invention relates to the technical field of inorganic phosphorus detection, in particular to an inorganic phosphorus detection reagent and a detection chip. Background technique [0002] Phosphorus has important physiological functions in the human body. Most of the phosphorus in the human body is deposited in the bones in the form of calcium phosphate, which is an important component of bones and teeth. Only a small portion of phosphorus is present in body fluids. Phosphorus in blood exists in two forms: inorganic phosphorus and organic phosphorus. Among them, inorganic phosphorus mainly exists in the form of phosphate, while a small part of phosphorus forms important organic compounds with other substances. The main physiological function of phosphorus is to participate in the metabolism of carbohydrates, lipids and amino acids, and constitute substances for energy operation. [0003] Inorganic phosphorus is usually measured in serum. At pres...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/3103
Inventor 周慧欣汪晨宇牟健陈明
Owner GENRUI BIOTECH INC