Aflatoxin detoxification composition as well as preparation method and application thereof

A technology for aflatoxin and composition, applied in the field of aflatoxin detoxification composition and preparation thereof, can solve the problems of easy formation of other harmful substances, generation of harmful substances, and seldom application, etc., and achieves increasing daily weight gain and improving growth. Condition, effect of relieving symptoms of poisoning

Pending Publication Date: 2021-10-22
HENAN PUAI FEED GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical methods: including chlorination, oxidation, hydrolysis and ammoniation; due to the disadvantages of environmental pollution and easy formation of other harmful substances in chemical treatment, they are rarely used in production
[0005] In summary, the existing detoxification agents and methods for aflatoxins are unsafe, produce harmful substances, and pollute the environment, and need to be further improved.

Method used

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  • Aflatoxin detoxification composition as well as preparation method and application thereof
  • Aflatoxin detoxification composition as well as preparation method and application thereof
  • Aflatoxin detoxification composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]Embodiment 1, for degrading aflatoxin B 1 Screening of strains

[0040] 1. Activation of strains

[0041] Candida utilis preserved in the laboratory was inoculated into YPD medium, and cultured with shaking at 30°C and 180r / min for 24h. Bacillus subtilis was inoculated into LB liquid medium, and cultured with shaking at 37°C and 180r / min for 24h.

[0042] Lactobacillus (Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus plantarum) was inoculated into MRS liquid medium, and cultured at a constant temperature of 37° C. for 24 hours. After measuring the number of viable bacteria by plate coating method, the number of viable bacteria was uniformly adjusted to 1×10 9 Colonyformed unit (CFU) / mL, the number of live bacteria is expressed by natural logarithm value (lg), and the strains are stored in a refrigerator at 4°C for later use.

[0043] 1.2 Screening for degraded AFB 1 single bacteria

[0044] 1.2.1 Experimental design and experimental grouping

[0045]...

Embodiment 2

[0055] Embodiment 2, the application of detoxification composition

[0056] 1 Feeding experiment design and grouping

[0057] The experiment was carried out at the Xuchang Experimental Base of Henan Agricultural University. 200 healthy AA broilers were selected and randomly divided into 4 treatment groups, with 5 repetitions in each group and 10 chickens in each repetition. The feeding period is 21d. Adopt multi-layered cages, feed freely, light 24h in the first week, (after one week, 23h light, 1h dark); the control of temperature and humidity is carried out according to routine feeding and management process; Immunization program is: when broiler chicken 7d, with new - Immunization with a dual vaccine. The diet was formulated according to the NRC (1994) broiler feeding standard, and the feed formula and nutritional level are shown in Table 2. The test groups are as follows:

[0058] Group A: basal diet (positive control, AFB in the diet 1 3.04 μg / kg);

[0059] Group B...

Embodiment 3

[0105] Example 3, the composition for detoxification on broiler liver and jejunum structure and microbial flora

[0106] Test reagent: formalin solution.

[0107] 1 Collection of test materials

[0108] During the 21-day slaughter test, in 3 treatment groups (A, B, D), 6 chickens were selected for slaughter (half male and female) in each group, and the same part of the jejunum was taken about 1.5 cm, and the same part of the liver was taken with a weight of 5 g. Tissues were rinsed with saline and soaked in 10% formalin. The contents of the jejunum were placed in 2 mL sterile cryopreservation tubes and stored in liquid nitrogen.

[0109] 1.1 Tissue section making and microscope observation

[0110] After the samples were fixed, they were sent to the Department of Pathology, Henan Agricultural University for testing.

[0111] 2.1 Effects of different treatment groups on the tissue structure of broiler liver and jejunum

[0112] Tissue section of broiler liver see figure 1...

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Abstract

The invention discloses an aflatoxin detoxification composition as well as a preparation method and application thereof. Active ingredients of the aflatoxin detoxification composition are glycyrrhizic acid and lactobacillus casei. The richness, diversity and flora structure information of the microbial flora are obtained through a 16S rRNA gene sequencing technology, and the result shows that mycotoxin breaks the balance of the intestinal flora, resulting in disorder of the intestinal flora; the ratio of the Rubrivivax and the Brevundimonas-intermedia is reduced by increasing the Novosphingobium-capsulatum through the combination of the glycyrrhizic acid and the lactobacillus casei so as to repair the disordered intestinal flora environment, such that the growth condition of the broiler chicken is improved, and the production performance is improved;.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an aflatoxin detoxification composition and a preparation method and application thereof. Background technique [0002] The word Mycotoxin appeared for the first time in 1960, when the turkeys on the turkey farm in the suburbs of London, England, just started to lose their appetite, and died one after another after two or three months. The autopsy found that it was caused by liver hemorrhage, necrosis and kidney swelling. die. After inspection and analysis, it was found that the cause of the fatality of the turkeys was that the workers fed moldy peanut powder to the turkeys, which was finally determined to be aflatoxin (Aflatoxin, AF). Aflatoxins are secondary metabolites of Aspergillus parasitica and Aspergillus flavus, which are specifically divided into more than a dozen toxins and more than 20 derivatives, named B 1 , B 2 , G 1 , G 2 Wait. [0003] Aflatoxin B 1 (Aflatoxin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61K31/704A61P39/02A61P1/00A23K50/75A23K10/18A23K20/121A23L5/20
CPCA61K35/747A61K31/704A61P39/02A61P1/00A23K50/75A23K10/18A23K20/121A23L5/28A23L5/27A23V2002/00A61K2300/00A23V2200/32
Inventor 尹清强赵闪闪常娟王平王利军卢富山王潇朱群
Owner HENAN PUAI FEED GRP CO LTD
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