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Lactobacillus rhamnosus NSL0401 with defecation promoting function and application thereof

A Lactobacillus rhamnosus, functional technology, applied in the field of microorganisms, can solve the problems of high price of hydrotherapy equipment and health hazards, and achieve the effects of improving defecation parameters, increasing the frequency of defecation, and enhancing intestinal function and health.

Active Publication Date: 2021-10-26
青岛诺森生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, intestinal hydrotherapy is mostly used, but this therapy has the disadvantages of needing to go to professional medical institutions for treatment, or the hydrotherapy equipment used at home is relatively expensive, and blind use will cause certain health risks

Method used

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  • Lactobacillus rhamnosus NSL0401 with defecation promoting function and application thereof
  • Lactobacillus rhamnosus NSL0401 with defecation promoting function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Isolation and identification of Lactobacillus rhamnosus NSL0401

[0021] 1. Separation and screening

[0022] Take pickles from 3 traditional Sichuan farmhouses as samples. Three portions of pickle juice were inoculated into MRS liquid medium at a volume of 1%, cultured at 37°C for 16 hours, and the culture solution was gradually diluted to 10 -8 . Choose 3 appropriate dilutions to take 1mL respectively, pour the modified MRS method onto the plate, culture anaerobically at 37°C for 48 hours, observe the colony morphology, select single colonies of different shapes and separate them by streaking on the modified MRS solid medium again, 37 Cultivate anaerobically at ℃ for 48 hours, repeat streaking and separation for 3 generations, obtain purified strains, carry out Gram staining and microscopic observation, and select the strains that are positive in Gram staining, spherical, rod-shaped, and multi-shaped by microscopic observation. A strain numbered NSL0401 ...

Embodiment 2

[0036] Example 2: Safety Evaluation of Lactobacillus rhamnosus NSL0401

[0037] 1. Hemolytic test

[0038] Detection of hemolytic activity of strains Hemolysis refers to the rupture of red blood cells and the escape of hemoglobin under the action of hemolytic toxin and other physical and chemical factors. The hemolytic activity of strain metabolites is considered to be an important index for the safety evaluation of strains.

[0039] Inoculate the activated Lactobacillus rhamnosus NSL0401 and the quality control strain Escherichia coli on the Columbia blood agar plate with a sterilized inoculation loop, culture at 37°C for 48 hours, and do a blank test at the same time to observe whether there is any A hemolytic circle appears.

[0040] The results showed that there was no obvious hemolytic circle formed around Lactobacillus rhamnosus NSL0401, which belonged to non-toxic γ-hemolysis, which also indicated that Lactobacillus rhamnosus NSL0401 had no pathogenicity and good safe...

Embodiment 3

[0044] Example 3: Effect of Lactobacillus rhamnosus NSL0401 on a mouse model of flaccid constipation

[0045] 1. Mouse experiment

[0046] After activating the preserved Lactobacillus rhamnosus NSL0401, inoculate it in a modified MRS liquid medium, and culture it anaerobically at 37°C for 48 hours to obtain a fermentation broth with a bacterial content of not less than 1×10 7 CFU / mL for subsequent experiments.

[0047]Thirty purchased male C57BL / 6 mice were randomly divided into control group, tegaserod maleate group and Lactobacillus rhamnosus NSL0401 group, and the mice were allowed to eat and drink freely. Among the 3 groups of mice, the control group was given normal broth medium, and the other 2 groups were given tegaserod maleate distilled water solution (Tegaserod, referred to as T) and Lactobacillus rhamnosus NSL0401 fermentation broth, respectively, and were given intragastric administration. deal with. The mice were bred for 7 days. After 7 days, the defecation pa...

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Abstract

The invention discloses a Lactobacillus rhamnosus NSL0401 with a defecation promoting function and an application thereof. The Lactobacillus rhamnosus NSL0401 is classified and named as Lactobacillus rhamnosus and is preserved in the China Center for Type Culture Collection, the preservation number is CTCC M 2021627, and the nucleotide sequence is as shown in SEQ ID No.1. The Lactobacillus rhamnosus NSL0401 is free of pathogenicity, free of acute toxicity, safe and reliable, and experiments prove that the Lactobacillus rhamnosus NSL0401 can effectively improve defecation parameters of an organism, increase defecation times and the water content of excrement, promote the variety and proliferation of probiotics in the organism and increase the concentration of various short-chain fatty acids, so that the effects of promoting defecation, relieving or treating achalasia constipation are realized, and the intestinal function and health condition of the organism are enhanced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Lactobacillus rhamnosus NSL0401 with the function of promoting defecation and its application. Background technique [0002] Flaccid constipation belongs to functional constipation, which is due to systemic factors or related defecation muscle weakness and low tension, especially low intestinal smooth muscle tension, intestinal motility relaxation, etc., resulting in insufficient or insufficient defecation power, and intestinal contents are transported too much in the colon. Constipation that develops slowly. At present, intestinal hydrotherapy is mostly used, but this therapy has the disadvantages of needing to go to a professional medical institution for treatment, or the hydrotherapy equipment used at home is relatively expensive, and blind use will cause certain health risks. [0003] Probiotics are a type of active microorganisms that are bene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/10C12P7/52C12P7/40C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P1/10C12P7/52C12P7/40A23V2002/00A23V2400/175A23V2200/3204Y02A50/30
Inventor 马超李昊彦刘翠玲孙海燕张明俊赵志强
Owner 青岛诺森生物技术有限责任公司
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