Unlock instant, AI-driven research and patent intelligence for your innovation.

Cervical cancer immunohistochemical analysis kit and preparation method thereof

A technology of immunohistochemistry and analytical reagents, which is applied in the direction of material analysis, material stimulation analysis, and material analysis through optical means. It can solve the problems of difficult to obtain materials, easy to miss diagnosis and misdiagnosis, and reduce accuracy, so as to improve detection accuracy. And the detection efficiency, repeatability and accuracy are good, and the effect of reducing uncontrollable errors

Pending Publication Date: 2021-10-26
武汉康义盛生物科技有限公司
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, cervical cancer screening generally adopts a three-step diagnosis of cervical lesions (liquid-based cytology, colposcopy and histopathological diagnosis) combined with HPV detection, but early diagnosis of cervical cancer is still difficult
There are three main reasons for this: (1) Liquid-based cytology examination does not clearly distinguish the grade of cervical cytopathy, and is greatly affected by subjective factors, such as the overlap of blood and mucus cells will affect the identification of abnormal cells , reducing the accuracy, leading to a high false negative rate, and easy misdiagnosis and misdiagnosis; (2) Both colposcopy and histopathological diagnosis are invasive examination methods, and at the same time, it is difficult to obtain materials in place, and it is difficult to obtain typical and sufficient pathological specimens (3) Although the sensitivity of HPV DNA detection is high, the specificity is low, and its positive result can only indicate whether there is a transient infection, and the value of predicting the occurrence and progression of cervical cancer needs to be further confirmed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cervical cancer immunohistochemical analysis kit and preparation method thereof
  • Cervical cancer immunohistochemical analysis kit and preparation method thereof
  • Cervical cancer immunohistochemical analysis kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] A preparation method of cervical cancer immunohistochemical analysis kit, comprising the following steps:

[0025] (1) Preparation of the metal nano-ion solution: adding the metal nano-particle solution or the metal oxide nano-particle solution or the metal compound particle solution into the PBS buffer solution, shaking to disperse the metal nanoparticles evenly. Wherein, the metal used is a metal nanoparticle that can undergo a coordination reaction with the antibody, and the particle size of the metal nanoparticle is generally controlled between 10 and 1000 nm. For example, the metal nanoparticle can be titanium nanoparticle or copper nanoparticle or Nickel nanoparticles or silver nanoparticles or cadmium nanoparticles or aluminum nanoparticles or platinum nanoparticles or cobalt nanoparticles, the compound can be NaAuCl 4 , AgNO 3 and CuCl 2 compounds etc.

[0026] (2) Preparation of metal-primary antibody composite nanoparticles I: Add monoclonal antibody or pol...

Embodiment 1

[0031] This embodiment provides a method for preparing a cervical cancer immunohistochemical analysis kit, which specifically includes the following steps:

[0032] (1) Preparation of metal nanoparticles solution: absorb 100 μL of metal nanoparticles solution (concentration of metal particles is 0.5 M), add to 100 mL of PBS buffer, vortex to disperse metal nanoparticles evenly, and obtain metal nanoparticles solution. The metal particles used can be titanium nanoparticles, copper nanoparticles, nickel nanoparticles, silver nanoparticles, cadmium nanoparticles, aluminum nanoparticles, platinum nanoparticles or cobalt nanoparticles, and the particle diameter of the metal particles used is 10-1000nm. PBS formula (pH7.4): Weigh potassium dihydrogen phosphate (KH 2 PO 4 )0.24g, disodium hydrogen phosphate (Na 2 HPO 4 ) 1.44g, sodium chloride (NaCl) 8g, potassium chloride (KCl) 0.2g, add about 800mL of particle-free water and stir to dissolve, then add concentrated hydrochloric a...

Embodiment 2

[0039] Application of embodiment 2 kit

[0040] 2.1 Production of manual smears:

[0041] (1) Put the vial of cervical cell liquid-based fixative on the mixer and shake it fully for 1-3 minutes;

[0042] (2) Draw out 10 mL of the shaken cell fixative solution with a syringe, and slowly add it along the tube wall into the conical tube pre-added with 1 mL of ethanol-agar solution;

[0043] (3) Centrifuge for 10 minutes, and transfer all the cell components to about 0.5-1 mL of ethanol-agar at the bottom of the tube;

[0044] (4) Pour off the supernatant, add cell buffer, place the conical tube on the mixer and shake it fully for 1 min;

[0045] (5) Draw 200-300 μL of cell buffer at the bottom of the tube with a sample gun, drop it on the center of the slide, let it settle for 20 minutes, pour off the supernatant, and cover with 2 / 3 of another slide On the ethanol-agar of the previous slide, the two slides intersect vertically, and pull the cover slide at a constant speed unti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of biological detection, in particular to a cervical cancer immunohistochemical analysis kit. The kit comprises metal-primary antibody composite nanoparticles I, metal-secondary antibody composite nanoparticles II, an antigen repair liquid and a cleaning liquid, wherein the secondary antibody contains a Raman active substance mark. According to the immunohistochemical analysis kit for the cervical cancer, provided by the invention, a biosensor technology for enhancing Raman scattering characteristics on the surface of metal nanoparticles is combined with immunohistochemical diagnosis, so that the problems in the prior art that manual reading is time-consuming and labor-consuming and is influenced by cognitive competence and experience of doctors can be effectively solved; the problem that uncontrollable errors easily occur during manual film reading is reduced, and the detection accuracy and the detection efficiency are improved.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a cervical cancer immunohistochemical analysis kit. Background technique [0002] Cervical cancer ranks second in the incidence of female cancers in the world and is an important public health problem. There are about 500,000 new cervical cancer pathologies in the world every year, and 273,000 people die from cervical cancer. There are about 135,000 new cervical cancer cases in China every year, accounting for 1 / 3 of the new cases in the world. Therefore, China is also a high incidence of cervical cancer one of the countries. [0003] At present, cervical cancer screening generally uses a three-step diagnosis of cervical lesions (liquid-based cytology, colposcopy, and histopathological diagnosis) combined with HPV detection, but early diagnosis of cervical cancer is still relatively difficult. There are three main reasons for this: (1) Liquid-based cytology examina...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577G01N33/58G01N33/532G01N21/65
CPCG01N33/57411G01N33/57484G01N33/577G01N33/587G01N33/532G01N21/658
Inventor 冯瑾瑾
Owner 武汉康义盛生物科技有限公司