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Medicine used for tumor treatment

A tumor treatment and drug technology, applied in the direction of anti-tumor drugs, drug combination, drug delivery, etc., can solve the problems of high incidence of side effects, large side effects, easy to produce drug resistance, etc., and achieve the effect of tumor suppression and metastasis inhibition

Pending Publication Date: 2021-10-29
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radiotherapy and chemotherapy are broad-spectrum effective, but have relatively large side effects; various small-molecule inhibitor therapies are targeted, but are expensive and prone to drug resistance; immunotherapies such as PD1 antibody and PDL1 antibody are effective, but there are few suitable populations ; The effect of CAR-T therapy is remarkable, but the incidence of side effects is high

Method used

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  • Medicine used for tumor treatment
  • Medicine used for tumor treatment
  • Medicine used for tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Preparation of ORFV120 gene deletion strain:

[0051] The ORFV120 gene deletion strain of the present invention is prepared in the following manner:

[0052] (1) Prepare the parental strain: the parental strain is the ORFV-SY17 strain of the ovine infectious impetigo virus, and the full-length gene sequence of the ORFV-SY17 strain of the ovine infectious impetigo virus is shown in GenBank: MG712417.1 ;

[0053] (2) Construction of the screening expression cassette: inserting the specific promoter and the first marker gene suitable for the sheep infectious impetigo virus into the vector backbone to obtain the screening expression cassette;

[0054] (3) Construction of the shuttle plasmid: amplify the sequences on both sides of the ORFV120 gene deletion sequence of the ORFV-SY17 strain as the recombination homology arm, clone it into the screening expression cassette constructed in step (2), and obtain the marked recombination shuttle plasmid;

[0055] (4) using the shu...

Embodiment 2

[0074] Example 2 Detection of anti-tumor effect of ORFV120 gene deletion strain

[0075] In Example 2, the anti-tumor effect of the ORFV120 gene deletion strain obtained in Example 1 was tested.

[0076] 1. Transmission electron microscope observation and identification of ORFV120 gene deletion strain

[0077] The ORFV120 gene deletion strain was observed and identified under a transmission electron microscope. The result is as figure 1 show.

[0078] 2. Toxicity safety evaluation of ORFV120 gene deletion strain

[0079] During the implementation of the present invention, the ORFV120 gene deletion strain was intramuscularly injected into mice, and the injection dose was 2×10 6 TCID 50 , Record the body weight change of mice injected 0-15 days. The result is as figure 2 It was shown that the above viruses did not significantly change the body weight of mice.

[0080] 3. The effect of therapeutic injection of ORFV120 gene deletion strain on the recruitment of immune cel...

Embodiment 3

[0089] Example 3 The anti-tumor drug obtained in Example 1 is used in combination with at least one of anti-tumor radiotherapy, anti-tumor chemotherapy, anti-tumor immunity, anti-tumor targeting, and anti-tumor hormone drugs. The combined chemical, antibody drugs, targeted inhibitors and hormone drugs are administered by intraperitoneal injection or oral administration, and the antitumor drugs including the attenuated strain of sheep infectious impetigo virus of the present invention are administered at staggered time intervals.

[0090] Combined use with anti-tumor chemotherapy: intraperitoneal injection of Etoposide (etoposide is a cell cycle-specific anti-tumor drug with a molecular formula of C 29 h 32 o 13 ) 10-50mg / kg, once every two days; the drug for tumor treatment including the attenuated strain of sheep infectious impetigo virus takes the form of intratumoral injection, staggered with anti-tumor chemotherapeutic drugs, and the dose is 10 6 TCID 50 .

[0091] Com...

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Abstract

The invention provides a medicine used for tumor treatment. The medicine comprises an ORFV attenuated strain. A virulence gene deletion strain of the ORFV is prepared through deletion of virulence genes including ORFV001-ORFV008, ORFV020, ORFV024, ORFV073, ORFV112-ORFV131 and ORFV134, and the virulence gene deletion strain is the ORFV attenuated strain. The ORFV attenuated strain is used for anti-tumor drugs, prevents tumor cell growth, promotes tumor cell death, prevents in-situ tumor growth, prevents tumor metastasis, and increases the proportion of immune cells in the tumor.

Description

technical field [0001] The invention relates to a new application of the attenuated strain of the sheep infectious impetigo virus, in particular to the application of the gene deletion and attenuated strain of the sheep infectious impetigo virus in anti-tumor. Background technique [0002] Tumor disease is not only the second largest killer of human health, but also ranks first in the top ten causes of death of companion animals such as dogs and cats. It is an important hazard to both human and animal health. Not only that, immunotherapy and chemotherapy combination therapy for tumor diseases have added a heavy economic burden to people's lives. At present, personalized treatment plans for specific tumors emerge in an endless stream. Radiotherapy and chemotherapy are broad-spectrum effective, but have relatively large side effects; various small-molecule inhibitor therapies are targeted, but are expensive and prone to drug resistance; immunotherapies such as PD1 antibody an...

Claims

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Application Information

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IPC IPC(8): A61K35/768A61K45/06A61K9/19A61K9/10A61K9/00A61K39/395A61P35/00A61P35/04C12N7/04C12N15/39A61K31/7048A61K31/4439A61K31/138
CPCA61K35/768A61K45/06A61K9/19A61K9/10A61K9/0019A61K31/7048A61K39/39558A61K31/4439A61K31/138A61P35/00A61P35/04C12N7/00C07K14/005C12N2710/24221C12N2710/24222C12N2710/24234C12N2710/24261A61K2300/00
Inventor 关继羽赵魁贺文琦林静孙诗惠高飞
Owner JILIN UNIV
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