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Preparation method of anaerobic ammonia oxidizing bacteria dry powder bacterial agent

A technology of anaerobic ammonium oxidizing bacteria and dry powder, applied to bacteria, adding compounds to stimulate growth, fixed on/in organic carriers, etc., can solve the problems of undetectable red bacteria activity, nitrite nitrogen accumulation, Nitrate nitrogen accumulation and other problems, to achieve high bacterial activity recovery rate, strong resistance to hydraulic load and substrate concentration load impact ability, and reduce the effect of reaction time

Pending Publication Date: 2021-10-29
黑龙江红菌生物科技有限公司
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (1) The abundance of red bacteria in nature and in wastewater biological treatment systems is very low, and the activity of red bacteria is almost undetectable in the determination of conventional biological inoculation sludge
[0008] (2) Rhodobacter is an autotrophic bacterium with low cell yield and slow growth, making it extremely difficult to enrich and cultivate
[0009] (3) Anammox bacteria are extremely sensitive to temperature, pH, dissolved oxygen, ammonia nitrogen, and nitrate nitrogen. Any fluctuation or impact in operation control will cause accumulation of nitrate nitrogen and nitrite nitrogen, resulting in The activity of the bacteria is reduced, the treatment rate is reduced, and in severe cases, it may even cause the system to crash and restart

Method used

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  • Preparation method of anaerobic ammonia oxidizing bacteria dry powder bacterial agent
  • Preparation method of anaerobic ammonia oxidizing bacteria dry powder bacterial agent

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specific Embodiment approach 1

[0023] Specific embodiment one: the preparation method of a kind of anaerobic ammonium oxidizing bacteria dry powder inoculant in this embodiment, carries out according to the following steps:

[0024] 1. Inject the mixed sludge and anaerobic medium into the anaerobic ammonium oxidation reaction device, then add manganese dioxide, activated carbon and bacteria growth promoter, and adjust the pH to 6.7-8.3, and then in 20-43 ℃ Start the anammox reaction device under temperature conditions, enrich for 1.5-2 days, and obtain the enriched anammox bacteria sample, and the volume ratio of the mixed sludge to the anaerobic medium is (0.05-0.1):1 , the mixed sludge is composed of anaerobic sludge and river bottom sludge, and the anaerobic medium is composed of 30mg / L KH 2 PO 4 , 500mg / L KHCO 3 , 200mg / L MgSO 4 , 100mg / L FeCl 3 、30mg / L CaCl 2 , 860mg / LCH 3 COONa, 660mg / L (NH 4 ) 2 SO 4 and 850mg / L NaNO 3 composition;

[0025] 2. Centrifuge the enriched anammox bacteria sampl...

specific Embodiment approach 2

[0033] Embodiment 2: This embodiment differs from Embodiment 1 in that: the volume ratio of anaerobic sludge to river bottom sludge in the mixed sludge described in step 1 is 3:1.

[0034] Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0035] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the quality of manganese dioxide in step one, the quality of gac and the ratio of the quality of bacterial growth promoter and the volume of mixed sludge are 1g: 1g: 2g: 3mL.

[0036] Other steps are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of an anaerobic ammonia oxidizing bacteria dry powder bacterial agent, and relates to the technical field of water treatment. The invention aims to solve the problems that the anaerobic ammonia oxidizing bacteria have a very small volume and are very likely to lose when being subjected to enrichment culture in a common bioreactor, and the like. The method comprises the following steps of: centrifuging an enriched anaerobic ammonia oxidizing bacteria sample, removing supernate, adding a protective agent, centrifuging again, and removing the supernate to obtain an anaerobic ammonia oxidizing bacteria precipitate; and mixing the anaerobic ammonia oxidizing bacteria precipitate with a sodium alginate solution, purging with inert gas, and finally, drying under a vacuum condition to obtain the anaerobic ammonia oxidizing bacteria dry powder bacterial agent, wherein the ratio of the mass of the anaerobic ammonia oxidizing bacteria precipitate to the volume of the sodium alginate solution is (100-150) g: (50-100) mL. The invention can obtain the preparation method of the anaerobic ammonia oxidizing bacteria dry powder bacterial agent.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a method for preparing an anammox bacteria dry powder bacterial agent. Background technique [0002] At present, the essence of sewage biological treatment is to decompose the organic matter in sewage through the metabolic activities of microorganisms, so as to achieve the purpose of purifying sewage. While improving water quality, sewage treatment also requires low energy consumption and low resource consumption. The complete autotrophic conversion of nitrogen combined with anaerobic ammonium oxidation and nitrosation is the most sustainable way to denitrify sewage. The most important one is the anammox bacteria. [0003] Since anammox bacteria are rich in cytochrome, when they become the dominant bacteria group, the mature anammox sludge will show a beautiful deep red color, and the change of sludge color can be used as an anammox reactor start-up Instructions for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C12N11/10
CPCC12N1/38C12N1/20C12N11/10
Inventor 徐善文赵立军
Owner 黑龙江红菌生物科技有限公司
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