Pathogenic microorganism detection method fused with spectrum technology

Abstract

The invention discloses a pathogenic microorganism detection method fused with a spectrum technology. The pathogenic microorganism detection method comprises the following steps: S1, preparing a to-be-detected sample and an active substrate; S2, mixing the active substrate with a to-be-detected sample according to a certain proportion, shaking up, and standing for 2-3 hours; s3, taking a proper amount of the mixed liquid, dripping the mixed liquid on a clean silicon wafer, and airing; s4, irradiating the silicon wafer through a Raman spectrometer, and collecting Raman spectrum information generated by microorganisms on the silicon wafer; s5, receiving and processing the Raman spectrum information to obtain a correlation peak graph, and displaying the correlation peak graph on the mobile terminal; and S6, detecting the types of microorganisms contained in the to-be-detected sample according to the obtained correlation peak map. The method has the advantages of fewer samples required for detection, difficulty in damage of the samples, lower detection cost, higher sensitivity, shorter time consumption and convenience in on-site and field detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pathogenic microorganism detection method combined with spectral technology. Background technique [0002] Microorganisms are a large group of organisms including bacteria, viruses, fungi, and some small protozoa. The individuals are tiny and closely related to human life. Widely involved in health, medicine, industry and agriculture, environmental protection and many other fields. In general, microorganisms are divided into the following eight categories: bacteria, viruses, fungi, actinomycetes, rickettsiae, mycoplasma, chlamydia, and spirochetes. [0003] For the detection of microorganisms, microscopes, staining techniques, culture medium preparation techniques, inoculation, separation and purification, and culture techniques are often used. However, when the above methods are used for processing, many samples are required and the probability of damaging the samples...

AI Technical Summary

Problems solved by technology

[0003] For the detection of microorganisms, microscopes, staining techniques, culture medium preparation techniques, inoculation, separation and purification, and culture techniques are often used. However, when the above methods are used for processing, many samples are required and the probability of damaging the samples is high, the cost is high, and the sensitivity is low. Low, the process is more complicated and time-consuming, which is not conducive to on-site and field detection