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Multi-strain combined microbial agent for improving soil and application thereof

A technology for improving soil and combining bacterial agents, applied in the restoration of contaminated soil, application, bacteria, etc., can solve the problems of excessive pesticides and fertilizers, lack of trace elements, unstable yields, etc., and achieve a simple cultivation method and production Effect

Pending Publication Date: 2021-11-05
YIZHITAI BIOTECH LONGYAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Problems in the prior art: the present invention will provide a soil bacterial agent that can solve the problems of excessive use of pesticides and chemical fertilizers, lack of trace elements in grains, and unstable yields

Method used

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  • Multi-strain combined microbial agent for improving soil and application thereof
  • Multi-strain combined microbial agent for improving soil and application thereof
  • Multi-strain combined microbial agent for improving soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present embodiment provides a kind of multi-species combined microbial agent for improving soil, including following 10 kinds of bacterial species:

[0041] (1) Pseudomonas fluorescens (P. Fluorescens), Acinetobacter baumannii (Acinetobacter baumannii), Bacillus licheniformis (Bacillus) and Bacillus subtilis (Bacillus subtilis).

[0042] (2) Agrobacterium radiobacter and Pasteurella.

[0043] (3) Lactobacillus, Azotobacter chroococcum, saccharomyces and Trichoderma harzianum.

Embodiment 2

[0045] This embodiment provides a kind of multi-strain separation, cultivation and preservation method for improving soil, comprising:

[0046](1) Isolation and identification of strains: Pseudomonas fluorescens (P.Fluorescens) was purchased from Ningbo Taisi Tuo Biotechnology Co., Ltd.; Bacillus licheniformis (Bacillus) and Bacillus subtilis (Bacillus subtilis) were purchased from Cangzhou Huayu Biotechnology Co., Ltd.; Lactobacillus, Lactobacillus and Agrobacterium radiobacter were purchased from Ningbo Mingzhou Biotechnology Co., Ltd.; Trichoderma harzianum was purchased from Yiyuan Hanlin Agricultural Development Co., Ltd.; Other strains were isolated from the soil of multi-year crop rotation (isolated and identified using the methods in references 1-5).

[0047] (2) Medium configuration: medium 1: 40g white sugar; 15g peptone; 25g yeast powder; 5g sodium acetate; 2g potassium dihydrogen phosphate; 2g sodium citrate; 0.2g magnesium sulfate; 1000g water; adjust the pH to 6....

Embodiment 3

[0057] Present embodiment provides a kind of application of the multi-strain combination bacterial agent of improving soil, including:

[0058] 1. Selection of sample plots and crops: Select the soil that has been rotated for many years and has no pests and diseases as the sample plot. The standard size of 1 hectare is divided into 10 plots. Three different provinces (Tianshui, Gansu, Luoyang, Henan, and Tai’an, Shandong) are tested at the same time. Spring wheat (Xinchun 6), potato (Qingshu 9), corn (Nonghua 101) and soybean (Changjiangchun 1) were planted respectively.

[0059] 2. Bacteria treatment: One week before spring wheat, potato, corn and soybean sowing, the soil was sprayed with bacteria agents 1-5 (different bacteria agents for different plots) before rotary tillage, and the soil was rotated immediately after spraying. The control group was not sprayed with fungicide, and the others were the same.

[0060] 3. Field management: conventional weeding in the field, no...

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Abstract

The invention provides a multi-strain combined microbial agent for improving soil. The multi-strain combined microbial agent comprises one or more of the following ten strains: pseudomonas fluorescens, acinetobacter baumannii, bacillus licheniformis, bacillus subtilis, agrobacterium radiobact, pasteurella multocide, lactic acid bacillus, azotobacter chroococcum, saccharomycetes and trichoderma harzianum. The multi-strain combined microbial agent for improving the soil, which is provided by the invention, integrates physiological advantages of various strains, is simple in culture method and preparation, and can significantly increase the yield of spring wheat, corn, potatoes and soybeans; and the combination of partial strains can significantly increase the content of trace elements (iron, manganese and selenium) in the spring wheat, the corn, the potatoes and the soybeans.

Description

technical field [0001] The invention relates to a soil modifier, in particular to a soil-improving multi-strain combination bacterial agent and a preparation method thereof. Background technique [0002] Cultivated land is the basic resource and condition for human survival. Since the 1950s, the population has entered a period of rapid growth, but the cultivated land has gradually decreased, and people's living standards have continued to improve. To maintain sustainable agricultural development, we must first ensure the quantity and quality of cultivated land. According to incomplete statistics from UNESCO and the Food and Agriculture Organization (FAO), the world's land area is about 1.829 billion hectares, and the per capita cultivated land is 0.26 hectares. The amount of cultivated land in my country is 1.82476 billion mu. [0003] my country began to develop and utilize land resources in the 1950s, focusing on research on soil fertility related to productivity improvem...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14B09C1/10A01N63/22A01N63/20A01N63/32A01N63/38A01P21/00C12R1/39C12R1/10C12R1/125C12R1/01C12R1/225C12R1/065C12R1/85C12R1/885
CPCC12N1/20C12N1/16C12N1/14B09C1/105A01N63/22A01N63/20A01N63/32A01N63/38B09C2101/00
Inventor 严建彬严俊文
Owner YIZHITAI BIOTECH LONGYAN CO LTD
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