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Preservation method of lactic acid bacteria

A technology for preservation of lactic acid bacteria and strains, applied in the field of preservation of microorganism strains, can solve the problems of large space occupied by preservation, high frequency of strain variation, unsuitability for lactic acid bacteria, etc., and achieves long storage period, long storage time, and is conducive to the adsorption of microorganisms. effect of cells

Active Publication Date: 2021-11-09
辽宁省微生物科学研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid paraffin preservation method is only suitable for slant and puncture culture, but the slant culture method is not suitable for the cultivation of lactic acid bacteria due to the large contact surface between the slant medium and the air, and the puncture culture is inconvenient to operate when the bacteria are activated.
Freeze-drying and liquid nitrogen cryopreservation methods are complicated to operate, high in technical content, high in equipment investment, and high in operating costs. They are only suitable for professional preservation institutions, not for production enterprises.
The liquid culture in the regular transplantation preservation method is a relatively common preservation method for lactic acid bacteria. However, since the liquid culture must be placed vertically in a test tube rack, the storage takes up a lot of space. The most important thing is that the liquid culture needs to be subcultured every two months or so. , otherwise the strains will die, which will not only increase the workload, but also cause the frequent generation of strains, high frequency of strain mutation, and weakened vitality

Method used

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  • Preservation method of lactic acid bacteria
  • Preservation method of lactic acid bacteria
  • Preservation method of lactic acid bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] a) Put 25g of cabbagebang granules in a conical flask, sterilize at 121°C for 10 minutes, and cool for later use;

[0061] b) Put the improved MRS medium in a 500mL Erlenmeyer flask, sterilize at 121°C for 15min and cool down for later use;

[0062] c) mixing the sterilized Baicaibang granules in step a) with the sterilized modified MRS medium in step b);

[0063] d) Inoculate Lactobacillus plantarum into the above mixture at an inoculum size of 1% (v / v) under sterile conditions, and culture at 38°C for 1 day;

[0064] e) The above-mentioned cultivated strains are transferred to a sterile centrifuge tube under aseptic conditions and centrifuged at 5000r / min for 10min, discard the supernatant, collect the cabbage gang particles, replace the centrifuge tube with a sterilized gauze plug, and put Dry in a drying oven at 42°C for 2 hours, then transfer to sterile EP tubes, and finally store in refrigerators at 4°C and -18°C respectively.

[0065] The ratio of the improved ...

Embodiment 2

[0067] a) Put 25g of cabbagebang granules in a conical flask, sterilize at 121°C for 10 minutes, and cool for later use;

[0068] b) Put the improved MRS medium in a 500mL Erlenmeyer flask, sterilize at 121°C for 15min and cool down for later use;

[0069] c) mixing the sterilized Baicaibang granules in step a) with the sterilized modified MRS medium in step b);

[0070] d) Inoculate Lactobacillus brevis into the above mixture at an inoculum size of 2% (v / v) under sterile conditions, and culture at 40°C for 1 day;

[0071] e) The above-mentioned cultivated strains are transferred to a sterile centrifuge tube under aseptic conditions and centrifuged at 5000r / min for 10min, discard the supernatant, collect the cabbage gang particles, replace the centrifuge tube with a sterilized gauze plug, and put Dry in a drying oven at 42°C for 2 hours, then transfer to sterile EP tubes, and finally store in refrigerators at 4°C and -18°C respectively.

[0072] The ratio of the improved MRS...

Embodiment 3

[0074] a) Put 25g of cabbagebang granules in a conical flask, sterilize at 121°C for 10 minutes, and cool for later use;

[0075] b) Put the improved MRS medium in a 500mL Erlenmeyer flask, sterilize at 121°C for 15min and cool down for later use;

[0076] c) mixing the sterilized Baicaibang granules in step a) with the sterilized modified MRS medium in step b);

[0077] d) Inoculate Lactobacillus paracasei into the above mixture at an inoculum size of 3% (v / v) under aseptic conditions, and culture at 42°C for 1 day;

[0078] e) The above-mentioned cultivated strains are transferred to a sterile centrifuge tube under aseptic conditions and centrifuged at 5000r / min for 10min, discard the supernatant, collect the cabbage gang particles, replace the centrifuge tube with a sterilized gauze plug, and put Dry in a drying oven at 42°C for 2 hours, then transfer to sterile EP tubes, and finally store in refrigerators at 4°C and -18°C respectively.

[0079] The ratio of the improved ...

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Abstract

The invention relates to the field of microbial strain preservation, in particular to a preservation method of lactic acid bacteria. Vegetables are adopted as a strain preservation carrier, lactic acid bacteria are inoculated into a mixed solution of vegetable particles and an improved MRS culture medium to be cultured, centrifugation is conducted after culture is completed, supernate is discarded, and the vegetable particles are collected and preserved in a sealed mode at the preservation temperature ranging from minus 20 DEG C to 4 DEG C. The preservation method overcomes the defects in the prior art, and provides a microbial strain preservation method which is suitable for production enterprises, long in preservation time, simple and practical to operate, stable and reliable in strain quality and low in preservation cost.

Description

technical field [0001] The invention relates to the field of microbial strain preservation, in particular to a method for preserving lactic acid bacteria. Background technique [0002] At present, microbial strains are usually preserved by regular transplantation, liquid paraffin preservation, freeze-drying, and liquid nitrogen cryopreservation. Preservation under low temperature conditions can slow down the metabolic activity of microbial strains, inhibit their reproduction speed, achieve the purpose of reducing strain mutations and prolonging the preservation time of strains. [0003] Regular transplant preservation method: It is a classic simple preservation method, also known as the descendant preservation method. The method includes slant culture, liquid culture and puncture culture. The advantage of this method is that it is easy to operate, but the disadvantage is that the contact surface between the slant medium and the air is large, the dehydration is fast, and it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/25C12R1/24C12R1/225
CPCC12N1/20C12N1/04
Inventor 刘晓辉李莉峰池景良李杨敖静高晓梅孙玉禄桓明辉于淼郭玲玲李鑫宗玉丽
Owner 辽宁省微生物科学研究院
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